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High hydrostatic pressure specifically affects molecular dynamics and shape of low-density lipoprotein particles

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ABSTRACT

Lipid composition of human low-density lipoprotein (LDL) and its physicochemical characteristics are relevant for proper functioning of lipid transport in the blood circulation. To explore dynamical and structural features of LDL particles with either a normal or a triglyceride-rich lipid composition we combined coherent and incoherent neutron scattering methods. The investigations were carried out under high hydrostatic pressure (HHP), which is a versatile tool to study the physicochemical behavior of biomolecules in solution at a molecular level. Within both neutron techniques we applied HHP to probe the shape and degree of freedom of the possible motions (within the time windows of 15 and 100 ps) and consequently the flexibility of LDL particles. We found that HHP does not change the types of motion in LDL, but influences the portion of motions participating. Contrary to our assumption that lipoprotein particles, like membranes, are highly sensitive to pressure we determined that LDL copes surprisingly well with high pressure conditions, although the lipid composition, particularly the triglyceride content of the particles, impacts the molecular dynamics and shape arrangement of LDL under pressure.

No MeSH data available.


Related in: MedlinePlus

Spectral weights or Q dependence of the integrated areas of the elastic peak and the Lorentzian functions on IN5 and IN6 for both pressure values.For 20 bar the data points are almost completely superposed.
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f7: Spectral weights or Q dependence of the integrated areas of the elastic peak and the Lorentzian functions on IN5 and IN6 for both pressure values.For 20 bar the data points are almost completely superposed.

Mentions: First, we calculated the hydrogen content of the different components of the samples (see Table 2) to get information about their corresponding scattering contribution and consequently about the ratios of the components which participate to the different motions. Second, we determined the normalised spectral weights of the scattering contributions of the different populations (see Fig. 7) for both instruments and pressures.


High hydrostatic pressure specifically affects molecular dynamics and shape of low-density lipoprotein particles
Spectral weights or Q dependence of the integrated areas of the elastic peak and the Lorentzian functions on IN5 and IN6 for both pressure values.For 20 bar the data points are almost completely superposed.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC5382586&req=5

f7: Spectral weights or Q dependence of the integrated areas of the elastic peak and the Lorentzian functions on IN5 and IN6 for both pressure values.For 20 bar the data points are almost completely superposed.
Mentions: First, we calculated the hydrogen content of the different components of the samples (see Table 2) to get information about their corresponding scattering contribution and consequently about the ratios of the components which participate to the different motions. Second, we determined the normalised spectral weights of the scattering contributions of the different populations (see Fig. 7) for both instruments and pressures.

View Article: PubMed Central - PubMed

ABSTRACT

Lipid composition of human low-density lipoprotein (LDL) and its physicochemical characteristics are relevant for proper functioning of lipid transport in the blood circulation. To explore dynamical and structural features of LDL particles with either a normal or a triglyceride-rich lipid composition we combined coherent and incoherent neutron scattering methods. The investigations were carried out under high hydrostatic pressure (HHP), which is a versatile tool to study the physicochemical behavior of biomolecules in solution at a molecular level. Within both neutron techniques we applied HHP to probe the shape and degree of freedom of the possible motions (within the time windows of 15 and 100 ps) and consequently the flexibility of LDL particles. We found that HHP does not change the types of motion in LDL, but influences the portion of motions participating. Contrary to our assumption that lipoprotein particles, like membranes, are highly sensitive to pressure we determined that LDL copes surprisingly well with high pressure conditions, although the lipid composition, particularly the triglyceride content of the particles, impacts the molecular dynamics and shape arrangement of LDL under pressure.

No MeSH data available.


Related in: MedlinePlus