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From rabbit antibody repertoires to rabbit monoclonal antibodies

View Article: PubMed Central - PubMed

ABSTRACT

In this review, we explain why and how rabbit monoclonal antibodies have become outstanding reagents for laboratory research and increasingly for diagnostic and therapeutic applications. Starting with the unique ontogeny of rabbit B cells that affords highly distinctive antibody repertoires rich in in vivo pruned binders of high diversity, affinity and specificity, we describe the generation of rabbit monoclonal antibodies by hybridoma technology, phage display and alternative methods, along with an account of successful humanization strategies.

No MeSH data available.


Related in: MedlinePlus

Oryctolagus cuniculus. Shown is an individual with b9 κ-light-chain allotype from a pedigreed non-inbred colony of rabbits developed and characterized at the US National Institute of Allergy and Infectious Diseases, National Institutes of Health. The rabbits are currently housed at a rabbitry in Stanwood, WA, USA, owned by R & R Research, Inc. and available for custom immunization and harvests of peripheral blood, spleen and bone marrow. (Photo courtesy of Dr Rose G. Mage).
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fig1: Oryctolagus cuniculus. Shown is an individual with b9 κ-light-chain allotype from a pedigreed non-inbred colony of rabbits developed and characterized at the US National Institute of Allergy and Infectious Diseases, National Institutes of Health. The rabbits are currently housed at a rabbitry in Stanwood, WA, USA, owned by R & R Research, Inc. and available for custom immunization and harvests of peripheral blood, spleen and bone marrow. (Photo courtesy of Dr Rose G. Mage).

Mentions: European rabbits (Oryctolagus cuniculus; Figure 1) have played an important role as animal models in immunology for many decades.1, 2 Today, rabbits are still a major source for a wide variety of monoclonal antibodies (mAbs) and polyclonal antibodies (pAbs) with broad utility. pAbs can be described as a set of different antibodies generated in response to a specific pathogen or antigen, generally targeting different epitopes. mAbs, on the other hand, contain a defined antigen-binding site (paratope) that typically binds with high affinity and specificity to only one epitope. From a pharmaceutical point of view, mAbs provide a molecularly defined and reproducible product, whereas pAbs are traditionally an imprecise mixture of different antibodies.3 As is the case for mouse and human mAbs, IgG is the most common isotype of rabbit mAbs (Figure 2).


From rabbit antibody repertoires to rabbit monoclonal antibodies
Oryctolagus cuniculus. Shown is an individual with b9 κ-light-chain allotype from a pedigreed non-inbred colony of rabbits developed and characterized at the US National Institute of Allergy and Infectious Diseases, National Institutes of Health. The rabbits are currently housed at a rabbitry in Stanwood, WA, USA, owned by R & R Research, Inc. and available for custom immunization and harvests of peripheral blood, spleen and bone marrow. (Photo courtesy of Dr Rose G. Mage).
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC5382564&req=5

fig1: Oryctolagus cuniculus. Shown is an individual with b9 κ-light-chain allotype from a pedigreed non-inbred colony of rabbits developed and characterized at the US National Institute of Allergy and Infectious Diseases, National Institutes of Health. The rabbits are currently housed at a rabbitry in Stanwood, WA, USA, owned by R & R Research, Inc. and available for custom immunization and harvests of peripheral blood, spleen and bone marrow. (Photo courtesy of Dr Rose G. Mage).
Mentions: European rabbits (Oryctolagus cuniculus; Figure 1) have played an important role as animal models in immunology for many decades.1, 2 Today, rabbits are still a major source for a wide variety of monoclonal antibodies (mAbs) and polyclonal antibodies (pAbs) with broad utility. pAbs can be described as a set of different antibodies generated in response to a specific pathogen or antigen, generally targeting different epitopes. mAbs, on the other hand, contain a defined antigen-binding site (paratope) that typically binds with high affinity and specificity to only one epitope. From a pharmaceutical point of view, mAbs provide a molecularly defined and reproducible product, whereas pAbs are traditionally an imprecise mixture of different antibodies.3 As is the case for mouse and human mAbs, IgG is the most common isotype of rabbit mAbs (Figure 2).

View Article: PubMed Central - PubMed

ABSTRACT

In this review, we explain why and how rabbit monoclonal antibodies have become outstanding reagents for laboratory research and increasingly for diagnostic and therapeutic applications. Starting with the unique ontogeny of rabbit B cells that affords highly distinctive antibody repertoires rich in in vivo pruned binders of high diversity, affinity and specificity, we describe the generation of rabbit monoclonal antibodies by hybridoma technology, phage display and alternative methods, along with an account of successful humanization strategies.

No MeSH data available.


Related in: MedlinePlus