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Involvement of ethylene and polyamines biosynthesis and abdominal phloem tissues characters of wheat caryopsis during grain filling under stress conditions

View Article: PubMed Central - PubMed

ABSTRACT

Severe water deficit (SD) severely limited the photo-assimilate supply during the grain-filling stages. Although the ethylene and polyamines (PAs) have been identified as important signaling molecules involved in stress tolerance, it is yet unclear how 1-Aminocylopropane-1-carboxylic acid (ACC) and PA biosynthesis involving wheat abdominal phloem characters mitigate SD-induced filling inhibition. The results obtained indicated that the SD down-regulated the TaSUT1 expression and decreased the activities of sucrose synthase (SuSase, EC2.4.1.13), ADP glucose pyrophosphorylase (AGPase, EC2.7.7.27), soluble starch synthase (SSSase, EC2.4.1.21), then substantially limited grain filling. As a result, increased ACC and putrescine (Put) concentrations and their biosynthesis-related gene expression reduced spermidine (Spd) biosynthesis under SD condition. And, the ACC and PA biosynthesis in inferior grains was more sensitive to SD than that in superior grains. Intermediary cells (ICs) of caryopsis emerged prematurely under SD to compensate for the weakened photo-assimilate transport functions of sieve elements (SEs). Finally, plasmolysis and nuclear chromatin condensation of phloem parenchyma cells (PPC) and membrane degradation of SEs, as well as the decreased ATPase activity on plasma membranes of ICs and PPC at the later filling stage under SD were responsible for the considerably decreased weight of inferior grains.

No MeSH data available.


Post-anthesis severe water deficit effects on the 1-Aminocyclopropane-l-carboxylate synthase expression and spermidine synthesis related gene expression in wheat grains.
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f3: Post-anthesis severe water deficit effects on the 1-Aminocyclopropane-l-carboxylate synthase expression and spermidine synthesis related gene expression in wheat grains.

Mentions: To verify the variations of ACC and PAs under SD determined by HLPC methods, expression analysis for genes encoding enzymes involved in ACC and PAs biosynthesis was performed. As shown in Fig. 3A,B, ACS expression level was significantly increased in response to SD at 7, 14, and 21 DPA, and was much higher in inferior than superior grains.


Involvement of ethylene and polyamines biosynthesis and abdominal phloem tissues characters of wheat caryopsis during grain filling under stress conditions
Post-anthesis severe water deficit effects on the 1-Aminocyclopropane-l-carboxylate synthase expression and spermidine synthesis related gene expression in wheat grains.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC5382545&req=5

f3: Post-anthesis severe water deficit effects on the 1-Aminocyclopropane-l-carboxylate synthase expression and spermidine synthesis related gene expression in wheat grains.
Mentions: To verify the variations of ACC and PAs under SD determined by HLPC methods, expression analysis for genes encoding enzymes involved in ACC and PAs biosynthesis was performed. As shown in Fig. 3A,B, ACS expression level was significantly increased in response to SD at 7, 14, and 21 DPA, and was much higher in inferior than superior grains.

View Article: PubMed Central - PubMed

ABSTRACT

Severe water deficit (SD) severely limited the photo-assimilate supply during the grain-filling stages. Although the ethylene and polyamines (PAs) have been identified as important signaling molecules involved in stress tolerance, it is yet unclear how 1-Aminocylopropane-1-carboxylic acid (ACC) and PA biosynthesis involving wheat abdominal phloem characters mitigate SD-induced filling inhibition. The results obtained indicated that the SD down-regulated the TaSUT1 expression and decreased the activities of sucrose synthase (SuSase, EC2.4.1.13), ADP glucose pyrophosphorylase (AGPase, EC2.7.7.27), soluble starch synthase (SSSase, EC2.4.1.21), then substantially limited grain filling. As a result, increased ACC and putrescine (Put) concentrations and their biosynthesis-related gene expression reduced spermidine (Spd) biosynthesis under SD condition. And, the ACC and PA biosynthesis in inferior grains was more sensitive to SD than that in superior grains. Intermediary cells (ICs) of caryopsis emerged prematurely under SD to compensate for the weakened photo-assimilate transport functions of sieve elements (SEs). Finally, plasmolysis and nuclear chromatin condensation of phloem parenchyma cells (PPC) and membrane degradation of SEs, as well as the decreased ATPase activity on plasma membranes of ICs and PPC at the later filling stage under SD were responsible for the considerably decreased weight of inferior grains.

No MeSH data available.