Limits...
Modified-live PRRSV subtype 1 vaccine UNISTRAIN ® PRRS provides a partial clinical and virological protection upon challenge with East European subtype 3 PRRSV strain Lena

View Article: PubMed Central - PubMed

ABSTRACT

Background: Western European porcine reproductive and respiratory syndrome virus (PRRSV) strains cause limited and mild clinical signs whereas more virulent strains are circulating in Eastern Europe. The emergence of such highly virulent strains in Western Europe might result in severe clinical problems and a financial disaster. In this context, the efficacy of the commercial modified-live PRRSV subtype 1 vaccine UNISTRAIN® PRRS was tested upon challenge with the East European subtype 3 PRRSV strain Lena.

Results: The mean duration of fever was shortened and the number of fever days was significantly lower in vaccinated pigs than in control pigs. Moreover, a lower number of vaccinated animals showed fever, respiratory disorders and conjunctivitis. The mean virus titers in the nasal secretions post challenge (AUC) were significantly lower in the vaccinated group than in the control group. The duration of viremia was slightly shorter (not significantly different) in the vaccinated group as compared to the control group.

Conclusions: Vaccination of pigs with the modified-live vaccine UNISTRAIN® PRRS provides a partial clinical and virological protection against the PRRSV subtype 3 strain Lena.

No MeSH data available.


Related in: MedlinePlus

Nasal viral shedding after challenge with PRRSV subtype 3 strain Lena. Bullets represent individual animals; lines represent the mean titer in each group. Solid bullets and solid line show the titer for the control group; open bullets and dashed line show the titer for the vaccinated group. Dotted line represents the detection limit for the test
© Copyright Policy - OpenAccess
Related In: Results  -  Collection

License 1 - License 2
getmorefigures.php?uid=PMC5382438&req=5

Fig4: Nasal viral shedding after challenge with PRRSV subtype 3 strain Lena. Bullets represent individual animals; lines represent the mean titer in each group. Solid bullets and solid line show the titer for the control group; open bullets and dashed line show the titer for the vaccinated group. Dotted line represents the detection limit for the test

Mentions: Significant differences were found in viral shedding in nasal secretions. Viral shedding was observed from 3 dpc in both groups (Fig. 4). Titers peaked between 3 and 7 dpc in the control group and at 5 dpc in the vaccinated group. Peak mean titers were 5.6 ± 0.8 log10 tissue culture infectious dose with 50 % end point (TCID50)/100 mg and 5.0 ± 0.3 log10 TCID50/100 mg, respectively. In the control group, virus shedding was observed until at least 28 dpc (end of the experiment) with one out of six pigs still shedding virus (1.5 log10 TCID50/100 mg). In the five vaccinated pigs, viral shedding was observed in all animals up till 10 dpc. After that, two animals shed virus up till 28 dpc. Significant differences in virus titers were found at 3, 7 and 10 dpc. Moreover, the AUC value of virus secretion was significantly lower in the vaccinated pigs (11.6 ± 3.5) than in the non-vaccinated pigs (18.4 ± 1.9).Fig. 4


Modified-live PRRSV subtype 1 vaccine UNISTRAIN ® PRRS provides a partial clinical and virological protection upon challenge with East European subtype 3 PRRSV strain Lena
Nasal viral shedding after challenge with PRRSV subtype 3 strain Lena. Bullets represent individual animals; lines represent the mean titer in each group. Solid bullets and solid line show the titer for the control group; open bullets and dashed line show the titer for the vaccinated group. Dotted line represents the detection limit for the test
© Copyright Policy - OpenAccess
Related In: Results  -  Collection

License 1 - License 2
Show All Figures
getmorefigures.php?uid=PMC5382438&req=5

Fig4: Nasal viral shedding after challenge with PRRSV subtype 3 strain Lena. Bullets represent individual animals; lines represent the mean titer in each group. Solid bullets and solid line show the titer for the control group; open bullets and dashed line show the titer for the vaccinated group. Dotted line represents the detection limit for the test
Mentions: Significant differences were found in viral shedding in nasal secretions. Viral shedding was observed from 3 dpc in both groups (Fig. 4). Titers peaked between 3 and 7 dpc in the control group and at 5 dpc in the vaccinated group. Peak mean titers were 5.6 ± 0.8 log10 tissue culture infectious dose with 50 % end point (TCID50)/100 mg and 5.0 ± 0.3 log10 TCID50/100 mg, respectively. In the control group, virus shedding was observed until at least 28 dpc (end of the experiment) with one out of six pigs still shedding virus (1.5 log10 TCID50/100 mg). In the five vaccinated pigs, viral shedding was observed in all animals up till 10 dpc. After that, two animals shed virus up till 28 dpc. Significant differences in virus titers were found at 3, 7 and 10 dpc. Moreover, the AUC value of virus secretion was significantly lower in the vaccinated pigs (11.6 ± 3.5) than in the non-vaccinated pigs (18.4 ± 1.9).Fig. 4

View Article: PubMed Central - PubMed

ABSTRACT

Background: Western European porcine reproductive and respiratory syndrome virus (PRRSV) strains cause limited and mild clinical signs whereas more virulent strains are circulating in Eastern Europe. The emergence of such highly virulent strains in Western Europe might result in severe clinical problems and a financial disaster. In this context, the efficacy of the commercial modified-live PRRSV subtype 1 vaccine UNISTRAIN® PRRS was tested upon challenge with the East European subtype 3 PRRSV strain Lena.

Results: The mean duration of fever was shortened and the number of fever days was significantly lower in vaccinated pigs than in control pigs. Moreover, a lower number of vaccinated animals showed fever, respiratory disorders and conjunctivitis. The mean virus titers in the nasal secretions post challenge (AUC) were significantly lower in the vaccinated group than in the control group. The duration of viremia was slightly shorter (not significantly different) in the vaccinated group as compared to the control group.

Conclusions: Vaccination of pigs with the modified-live vaccine UNISTRAIN® PRRS provides a partial clinical and virological protection against the PRRSV subtype 3 strain Lena.

No MeSH data available.


Related in: MedlinePlus