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Modified-live PRRSV subtype 1 vaccine UNISTRAIN ® PRRS provides a partial clinical and virological protection upon challenge with East European subtype 3 PRRSV strain Lena

View Article: PubMed Central - PubMed

ABSTRACT

Background: Western European porcine reproductive and respiratory syndrome virus (PRRSV) strains cause limited and mild clinical signs whereas more virulent strains are circulating in Eastern Europe. The emergence of such highly virulent strains in Western Europe might result in severe clinical problems and a financial disaster. In this context, the efficacy of the commercial modified-live PRRSV subtype 1 vaccine UNISTRAIN® PRRS was tested upon challenge with the East European subtype 3 PRRSV strain Lena.

Results: The mean duration of fever was shortened and the number of fever days was significantly lower in vaccinated pigs than in control pigs. Moreover, a lower number of vaccinated animals showed fever, respiratory disorders and conjunctivitis. The mean virus titers in the nasal secretions post challenge (AUC) were significantly lower in the vaccinated group than in the control group. The duration of viremia was slightly shorter (not significantly different) in the vaccinated group as compared to the control group.

Conclusions: Vaccination of pigs with the modified-live vaccine UNISTRAIN® PRRS provides a partial clinical and virological protection against the PRRSV subtype 3 strain Lena.

No MeSH data available.


Related in: MedlinePlus

VN antibody titers upon vaccination with PRRSV subtype 1 vaccine UNISTRAIN® PRRS and challenge with PRRSV subtype 3 strain Lena. Bullets represent individual animals; lines represent the mean titer in each group. Solid bullets and solid line show the titer for the control group; open bullets and dashed line show the titer for the vaccinated group. Dotted line represents the detection limit for the test
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Fig3: VN antibody titers upon vaccination with PRRSV subtype 1 vaccine UNISTRAIN® PRRS and challenge with PRRSV subtype 3 strain Lena. Bullets represent individual animals; lines represent the mean titer in each group. Solid bullets and solid line show the titer for the control group; open bullets and dashed line show the titer for the vaccinated group. Dotted line represents the detection limit for the test

Mentions: Figure 2 represents the evolution of the IPMA antibody titers during the course of the experiment in vaccinated and unvaccinated control pigs. All control pigs remained seronegative until challenge. The vaccinated pigs seroconverted within two weeks after vaccination with a titer of 3.2 ± 0.8 log10 at -7 dpc. After challenge, an increase in IPMA antibody titers was observed in all animals within two weeks. Figure 3 represents the evolution of the virus neutralizing (VN) antibody titers against PRRSV. In both vaccinated and control groups, VN antibodies against PRRSV LV were not detected before challenge. After challenge, VN antibodies against PRRSV Lena were only detected in one out of five vaccinated animals at very low titers (≤3 log2) at 21 dpc. A similar pattern in ELISA antibodies was observed as for IPMA antibodies.Fig. 2


Modified-live PRRSV subtype 1 vaccine UNISTRAIN ® PRRS provides a partial clinical and virological protection upon challenge with East European subtype 3 PRRSV strain Lena
VN antibody titers upon vaccination with PRRSV subtype 1 vaccine UNISTRAIN® PRRS and challenge with PRRSV subtype 3 strain Lena. Bullets represent individual animals; lines represent the mean titer in each group. Solid bullets and solid line show the titer for the control group; open bullets and dashed line show the titer for the vaccinated group. Dotted line represents the detection limit for the test
© Copyright Policy - OpenAccess
Related In: Results  -  Collection

License 1 - License 2
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getmorefigures.php?uid=PMC5382438&req=5

Fig3: VN antibody titers upon vaccination with PRRSV subtype 1 vaccine UNISTRAIN® PRRS and challenge with PRRSV subtype 3 strain Lena. Bullets represent individual animals; lines represent the mean titer in each group. Solid bullets and solid line show the titer for the control group; open bullets and dashed line show the titer for the vaccinated group. Dotted line represents the detection limit for the test
Mentions: Figure 2 represents the evolution of the IPMA antibody titers during the course of the experiment in vaccinated and unvaccinated control pigs. All control pigs remained seronegative until challenge. The vaccinated pigs seroconverted within two weeks after vaccination with a titer of 3.2 ± 0.8 log10 at -7 dpc. After challenge, an increase in IPMA antibody titers was observed in all animals within two weeks. Figure 3 represents the evolution of the virus neutralizing (VN) antibody titers against PRRSV. In both vaccinated and control groups, VN antibodies against PRRSV LV were not detected before challenge. After challenge, VN antibodies against PRRSV Lena were only detected in one out of five vaccinated animals at very low titers (≤3 log2) at 21 dpc. A similar pattern in ELISA antibodies was observed as for IPMA antibodies.Fig. 2

View Article: PubMed Central - PubMed

ABSTRACT

Background: Western European porcine reproductive and respiratory syndrome virus (PRRSV) strains cause limited and mild clinical signs whereas more virulent strains are circulating in Eastern Europe. The emergence of such highly virulent strains in Western Europe might result in severe clinical problems and a financial disaster. In this context, the efficacy of the commercial modified-live PRRSV subtype 1 vaccine UNISTRAIN® PRRS was tested upon challenge with the East European subtype 3 PRRSV strain Lena.

Results: The mean duration of fever was shortened and the number of fever days was significantly lower in vaccinated pigs than in control pigs. Moreover, a lower number of vaccinated animals showed fever, respiratory disorders and conjunctivitis. The mean virus titers in the nasal secretions post challenge (AUC) were significantly lower in the vaccinated group than in the control group. The duration of viremia was slightly shorter (not significantly different) in the vaccinated group as compared to the control group.

Conclusions: Vaccination of pigs with the modified-live vaccine UNISTRAIN® PRRS provides a partial clinical and virological protection against the PRRSV subtype 3 strain Lena.

No MeSH data available.


Related in: MedlinePlus