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Cytokine response to the RSV antigen delivered by dendritic cell-directed vaccination in congenic chicken lines

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ABSTRACT

Systems of antigen delivery into antigen-presenting cells represent an important novel strategy in chicken vaccine development. In this study, we verified the ability of Rous sarcoma virus (RSV) antigens fused with streptavidin to be targeted by specific biotinylated monoclonal antibody (anti-CD205) into dendritic cells and induce virus-specific protective immunity. The method was tested in four congenic lines of chickens that are either resistant or susceptible to the progressive growth of RSV-induced tumors. Our analyses confirmed that the biot-anti-CD205-SA-FITC complex was internalized by chicken splenocytes. In the cytokine expression profile, several significant differences were evident between RSV-challenged progressor and regressor chicken lines. A significant up-regulation of IL-2, IL-12, IL-15, and IL-18 expression was detected in immunized chickens of both regressor and progressor groups. Of these cytokines, IL-2 and IL-12 were most up-regulated 14 days post-challenge (dpc), while IL-15 and IL-18 were most up-regulated at 28 dpc. On the contrary, IL-10 expression was significantly down-regulated in all immunized groups of progressor chickens at 14 dpc. We detected significant up-regulation of IL-17 in the group of immunized progressors. LITAF down-regulation with iNOS up-regulation was especially observed in the progressor group of immunized chickens that developed large tumors. Based on the increased expression of cytokines specific for activated dendritic cells, we conclude that our system is able to induce partial stimulation of specific cell types involved in cell-mediated immunity.

Electronic supplementary material: The online version of this article (doi:10.1186/s13567-017-0423-8) contains supplementary material, which is available to authorized users.

No MeSH data available.


Single cytokine profiles of selected genes in progressor and regressor groups of immunized and non-immunized chickens. The expression value after challenge was normalized to the basic value expression in the day of challenge. For more cytokines, see Additional file 5.
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Fig6: Single cytokine profiles of selected genes in progressor and regressor groups of immunized and non-immunized chickens. The expression value after challenge was normalized to the basic value expression in the day of challenge. For more cytokines, see Additional file 5.

Mentions: When comparing single cytokine expression profiles between progressor and regressor immunized and non-immunized groups, several significant differences were found (see Additional file 5). A significant up-regulation of IL-2, IL-12, IL-15 and IL-18 expression was detected in immunized chickens of both regressor and progressor groups. Of these cytokines, IL-2 and IL-12 expression was most up-regulated 14 dpc, while IL-15 and IL-18 were most up-regulated 28 dpc (Figure 6; IL-12, IL-18). On the contrary, IL-10 expression was significantly down-regulated 14 dpc in all immunized groups of progressor chickens. Interestingly, we detected significant up-regulation of IL-17 in the group of immunized progressors and significant up-regulation of MIF and XCL1 in both groups of progressors 14 dpc. Surprisingly, down-regulation of LITAF, a putative chicken analog of TNFα, and up-regulation of iNOS were observed especially in the progressor group of immunized chickens that developed large tumors.Figure 6


Cytokine response to the RSV antigen delivered by dendritic cell-directed vaccination in congenic chicken lines
Single cytokine profiles of selected genes in progressor and regressor groups of immunized and non-immunized chickens. The expression value after challenge was normalized to the basic value expression in the day of challenge. For more cytokines, see Additional file 5.
© Copyright Policy - OpenAccess
Related In: Results  -  Collection

License 1 - License 2
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getmorefigures.php?uid=PMC5382389&req=5

Fig6: Single cytokine profiles of selected genes in progressor and regressor groups of immunized and non-immunized chickens. The expression value after challenge was normalized to the basic value expression in the day of challenge. For more cytokines, see Additional file 5.
Mentions: When comparing single cytokine expression profiles between progressor and regressor immunized and non-immunized groups, several significant differences were found (see Additional file 5). A significant up-regulation of IL-2, IL-12, IL-15 and IL-18 expression was detected in immunized chickens of both regressor and progressor groups. Of these cytokines, IL-2 and IL-12 expression was most up-regulated 14 dpc, while IL-15 and IL-18 were most up-regulated 28 dpc (Figure 6; IL-12, IL-18). On the contrary, IL-10 expression was significantly down-regulated 14 dpc in all immunized groups of progressor chickens. Interestingly, we detected significant up-regulation of IL-17 in the group of immunized progressors and significant up-regulation of MIF and XCL1 in both groups of progressors 14 dpc. Surprisingly, down-regulation of LITAF, a putative chicken analog of TNFα, and up-regulation of iNOS were observed especially in the progressor group of immunized chickens that developed large tumors.Figure 6

View Article: PubMed Central - PubMed

ABSTRACT

Systems of antigen delivery into antigen-presenting cells represent an important novel strategy in chicken vaccine development. In this study, we verified the ability of Rous sarcoma virus (RSV) antigens fused with streptavidin to be targeted by specific biotinylated monoclonal antibody (anti-CD205) into dendritic cells and induce virus-specific protective immunity. The method was tested in four congenic lines of chickens that are either resistant or susceptible to the progressive growth of RSV-induced tumors. Our analyses confirmed that the biot-anti-CD205-SA-FITC complex was internalized by chicken splenocytes. In the cytokine expression profile, several significant differences were evident between RSV-challenged progressor and regressor chicken lines. A significant up-regulation of IL-2, IL-12, IL-15, and IL-18 expression was detected in immunized chickens of both regressor and progressor groups. Of these cytokines, IL-2 and IL-12 were most up-regulated 14 days post-challenge (dpc), while IL-15 and IL-18 were most up-regulated at 28 dpc. On the contrary, IL-10 expression was significantly down-regulated in all immunized groups of progressor chickens at 14 dpc. We detected significant up-regulation of IL-17 in the group of immunized progressors. LITAF down-regulation with iNOS up-regulation was especially observed in the progressor group of immunized chickens that developed large tumors. Based on the increased expression of cytokines specific for activated dendritic cells, we conclude that our system is able to induce partial stimulation of specific cell types involved in cell-mediated immunity.

Electronic supplementary material: The online version of this article (doi:10.1186/s13567-017-0423-8) contains supplementary material, which is available to authorized users.

No MeSH data available.