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Sustained High Levels of Both Total and High Molecular Weight Adiponectin in Plasma during the Convalescent Phase of Haemorrhagic Fever with Renal Syndrome Are Associated with Disease Severity

View Article: PubMed Central - PubMed

ABSTRACT

Haemorrhagic fever with renal syndrome (HFRS) is characterised by an uncontrolled immune response that causes vascular leakage. Adiponectin (APN) is an adipocytokine involved in prorevascularisation and immunomodulation. To investigate the possible effects of APN in the pathogenesis of HFRS, total and high molecular weight (HMW) APN levels in the plasma of patients with HFRS were quantified using enzyme-linked immunosorbent assay (ELISA). Compared with those in healthy controls, the plasma total and HMW APN levels in patients were elevated to different degrees from the fever onset and remained high at the convalescent phase. Consistent with these results, western blot analysis additionally showed that low molecular weight (LMW), middle molecular weight (MMW), and HMW APN levels were all elevated and contributed to the elevation of the total APN level. Importantly, sustained high levels of total and HMW APN at the convalescent phase were significantly higher in patients with critical disease than those in patients with mild or moderate disease. Moreover, total and HMW APN levels negatively correlated with white blood cell count and positively correlated with platelet count and serum albumin level. These results may provide insights into understanding the roles of total and HMW APN in the pathogenesis of HFRS.

No MeSH data available.


Related in: MedlinePlus

Comparison of the total APN and HMW APN levels in the plasma of patients with HFRS belonging to four disease severity groups in the convalescent phase. Both (a) total APN and (b) HMW APN levels in the plasma of critical patients were significantly higher than those of patients with mild and moderate disease at the convalescent phase. The Mann–Whitney U test was used to determine the significance of the differences between the two groups, and the black lines represent the medians with the corresponding interquartile range. ∗p < 0.05; ∗∗∗p < 0.001.
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fig3: Comparison of the total APN and HMW APN levels in the plasma of patients with HFRS belonging to four disease severity groups in the convalescent phase. Both (a) total APN and (b) HMW APN levels in the plasma of critical patients were significantly higher than those of patients with mild and moderate disease at the convalescent phase. The Mann–Whitney U test was used to determine the significance of the differences between the two groups, and the black lines represent the medians with the corresponding interquartile range. ∗p < 0.05; ∗∗∗p < 0.001.

Mentions: Next, we compared the total and HMW APN levels in the plasma of patients with HFRS delineated into different disease severity groups. During the acute phase, there was no significant difference in the total or HMW APN levels in the plasma among the four severity groups (data not shown). During the convalescent phase, the median levels of the total and HMW APN levels with their IQRs were 14.39 (10.27–21.16) μg/mL and 2.60 (1.82–4.70) μg/mL in patients with mild disease, 10.58 (8.75–17.64) μg/mL and 3.09 (1.80–5.49) μg/mL in patients with moderate disease, 14.76 (12.11–22.14) μg/mL and 3.25 (1.57–4.83) μg/mL in patients with severe disease, and 25.52 (15.15–30.14) μg/mL and 4.17 (2.96–5.98) μg/mL in patients with critical disease. Both the total and HMW APN levels in the patients with critical disease were significantly higher than those in the patients with mild or moderate disease (p < 0.05) (Figures 3(a) and 3(b)). However, there was no significant difference between the mild, moderate, and severe groups. Generally, the more serious the condition during the acute phase, the higher the levels of total APN and HMW APN in the plasma during the convalescent phase.


Sustained High Levels of Both Total and High Molecular Weight Adiponectin in Plasma during the Convalescent Phase of Haemorrhagic Fever with Renal Syndrome Are Associated with Disease Severity
Comparison of the total APN and HMW APN levels in the plasma of patients with HFRS belonging to four disease severity groups in the convalescent phase. Both (a) total APN and (b) HMW APN levels in the plasma of critical patients were significantly higher than those of patients with mild and moderate disease at the convalescent phase. The Mann–Whitney U test was used to determine the significance of the differences between the two groups, and the black lines represent the medians with the corresponding interquartile range. ∗p < 0.05; ∗∗∗p < 0.001.
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Related In: Results  -  Collection

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Show All Figures
getmorefigures.php?uid=PMC5382360&req=5

fig3: Comparison of the total APN and HMW APN levels in the plasma of patients with HFRS belonging to four disease severity groups in the convalescent phase. Both (a) total APN and (b) HMW APN levels in the plasma of critical patients were significantly higher than those of patients with mild and moderate disease at the convalescent phase. The Mann–Whitney U test was used to determine the significance of the differences between the two groups, and the black lines represent the medians with the corresponding interquartile range. ∗p < 0.05; ∗∗∗p < 0.001.
Mentions: Next, we compared the total and HMW APN levels in the plasma of patients with HFRS delineated into different disease severity groups. During the acute phase, there was no significant difference in the total or HMW APN levels in the plasma among the four severity groups (data not shown). During the convalescent phase, the median levels of the total and HMW APN levels with their IQRs were 14.39 (10.27–21.16) μg/mL and 2.60 (1.82–4.70) μg/mL in patients with mild disease, 10.58 (8.75–17.64) μg/mL and 3.09 (1.80–5.49) μg/mL in patients with moderate disease, 14.76 (12.11–22.14) μg/mL and 3.25 (1.57–4.83) μg/mL in patients with severe disease, and 25.52 (15.15–30.14) μg/mL and 4.17 (2.96–5.98) μg/mL in patients with critical disease. Both the total and HMW APN levels in the patients with critical disease were significantly higher than those in the patients with mild or moderate disease (p < 0.05) (Figures 3(a) and 3(b)). However, there was no significant difference between the mild, moderate, and severe groups. Generally, the more serious the condition during the acute phase, the higher the levels of total APN and HMW APN in the plasma during the convalescent phase.

View Article: PubMed Central - PubMed

ABSTRACT

Haemorrhagic fever with renal syndrome (HFRS) is characterised by an uncontrolled immune response that causes vascular leakage. Adiponectin (APN) is an adipocytokine involved in prorevascularisation and immunomodulation. To investigate the possible effects of APN in the pathogenesis of HFRS, total and high molecular weight (HMW) APN levels in the plasma of patients with HFRS were quantified using enzyme-linked immunosorbent assay (ELISA). Compared with those in healthy controls, the plasma total and HMW APN levels in patients were elevated to different degrees from the fever onset and remained high at the convalescent phase. Consistent with these results, western blot analysis additionally showed that low molecular weight (LMW), middle molecular weight (MMW), and HMW APN levels were all elevated and contributed to the elevation of the total APN level. Importantly, sustained high levels of total and HMW APN at the convalescent phase were significantly higher in patients with critical disease than those in patients with mild or moderate disease. Moreover, total and HMW APN levels negatively correlated with white blood cell count and positively correlated with platelet count and serum albumin level. These results may provide insights into understanding the roles of total and HMW APN in the pathogenesis of HFRS.

No MeSH data available.


Related in: MedlinePlus