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GC-MS Based Metabolite Profiling, Antioxidant and Antimicrobial Properties of Different Solvent Extracts of Malaysian Plectranthus amboinicus Leaves

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ABSTRACT

This study evaluates the phytochemistry, antioxidant, and antimicrobial effects of Plectranthus amboinicus leaves extracted in different solvents. The methanol extract contained the highest total phenolic (94.37 ± 1.24 mg GAE/g) and flavonoid contents (26.90 ± 1.35 mg RE/g) and exhibited the highest DPPH scavenging activity (90.13 ± 3.32%) followed by the acetone extract (80.23 ± 3.26%) at 500 μg/mL concentration. Similarly, the highest ferric ion reduction potential (849.63 ± 30.95 μM of Fe (II)/g dry weight) was exhibited by the methanol extract followed by the acetone extract (695.92 ± 25.44 μM of Fe (II)/g dry weight). The methanol extract showed greater antimicrobial activity against all the tested pathogens (Bacillus subtilis, Methicillin-resistant Staphylococcus aureus, Pseudomonas aeruginosa, Escherichia coli, and Candida albicans). However, both hexane and acetone extracts failed to inhibit E. coli. S. aureus and C. albicans were more susceptible to all the extracts. Further, GC-MS analysis confirmed the occurrence of a total 46 phytocompounds in different solvent extracts. Some of the major compounds included carvacrol (37.7%), tetracontane (16.6%), squalene (15.6%), tetrapentacontane (13.7%), and Phytol (12.9%). In conclusion, extraction solvents influenced the recovery of phytocompounds and the highest pharmacological activities of the methanol extract could be correlated to the presence of additional bioactive compounds.

No MeSH data available.


FRAP assay of various solvent extracts of P. amboinicus.
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fig2: FRAP assay of various solvent extracts of P. amboinicus.

Mentions: However, DPPH scavenging activity of all extracts was inferior to the standard (ascorbic acid). Similar pattern of antioxidant activity was evidenced from FRAP assay method where the highest ferric ion reduction potential was exhibited by the methanol extract (849.63 ± 30.95 μM of Fe (II)/g dry weight) followed by acetone extract with 695.92 ± 25.44 μM of Fe (II)/g dry weight (Figure 2). The lowest FRAP activity was observed in hexane extract (376.98 ± 15.42 μM of Fe (II)/g dry weight). However, the superior activity was evidenced in the standard, α-tocopherol.


GC-MS Based Metabolite Profiling, Antioxidant and Antimicrobial Properties of Different Solvent Extracts of Malaysian Plectranthus amboinicus Leaves
FRAP assay of various solvent extracts of P. amboinicus.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC5382359&req=5

fig2: FRAP assay of various solvent extracts of P. amboinicus.
Mentions: However, DPPH scavenging activity of all extracts was inferior to the standard (ascorbic acid). Similar pattern of antioxidant activity was evidenced from FRAP assay method where the highest ferric ion reduction potential was exhibited by the methanol extract (849.63 ± 30.95 μM of Fe (II)/g dry weight) followed by acetone extract with 695.92 ± 25.44 μM of Fe (II)/g dry weight (Figure 2). The lowest FRAP activity was observed in hexane extract (376.98 ± 15.42 μM of Fe (II)/g dry weight). However, the superior activity was evidenced in the standard, α-tocopherol.

View Article: PubMed Central - PubMed

ABSTRACT

This study evaluates the phytochemistry, antioxidant, and antimicrobial effects of Plectranthus amboinicus leaves extracted in different solvents. The methanol extract contained the highest total phenolic (94.37 ± 1.24 mg GAE/g) and flavonoid contents (26.90 ± 1.35 mg RE/g) and exhibited the highest DPPH scavenging activity (90.13 ± 3.32%) followed by the acetone extract (80.23 ± 3.26%) at 500 μg/mL concentration. Similarly, the highest ferric ion reduction potential (849.63 ± 30.95 μM of Fe (II)/g dry weight) was exhibited by the methanol extract followed by the acetone extract (695.92 ± 25.44 μM of Fe (II)/g dry weight). The methanol extract showed greater antimicrobial activity against all the tested pathogens (Bacillus subtilis, Methicillin-resistant Staphylococcus aureus, Pseudomonas aeruginosa, Escherichia coli, and Candida albicans). However, both hexane and acetone extracts failed to inhibit E. coli. S. aureus and C. albicans were more susceptible to all the extracts. Further, GC-MS analysis confirmed the occurrence of a total 46 phytocompounds in different solvent extracts. Some of the major compounds included carvacrol (37.7%), tetracontane (16.6%), squalene (15.6%), tetrapentacontane (13.7%), and Phytol (12.9%). In conclusion, extraction solvents influenced the recovery of phytocompounds and the highest pharmacological activities of the methanol extract could be correlated to the presence of additional bioactive compounds.

No MeSH data available.