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Costal cartilage transplantation for treatment of growth plate injury in a rabbit model

View Article: PubMed Central - PubMed

ABSTRACT

Objectives: The purpose of this study is to evaluate the effects of allogenic costal cartilage transplantation on preventing bony bridge formation and angular deformities for the treatment of partial growth plate injury using a rabbit model.

Methods: An experimental model of partial growth injury was created by resecting the medial part of the proximal tibial growth plate in male six-week-old New Zealand White rabbits. The rabbits were divided into four groups: no surgery; no transplantation; bone wax transplantation; and allogenic costal cartilage transplantation. The angular deformities of the tibia and bony bridge were analysed using radiographs and microcomputed tomography, and the repair of the injured growth plate cartilage and bony bridge formation rate were histologically evaluated.

Results: On radiographic evaluation, the varus deformities in the costal cartilage group were significantly improved compared with the no transplantation group at four and eight weeks after operation and with the bone wax group at eight weeks after operation. Micro-computed tomography showed that the bony bridge formation was prevented in the bone wax and costal cartilage groups. Histological findings showed that the bony bridge formation in the bone wax and costal cartilage group was decreased. In addition, the growth plate was continuous and stained with safranin O and immunohistochemically stained for type II collagen.

Conclusion: Transplantation of costal cartilage improved angular deformities and decreased bony bridge formation in the partial growth plate injury. Costal cartilage might be a suitable graft for the treatment of growth plate injury.

No MeSH data available.


Representative histological appearance of the mid-coronal proximal left tibia. H&E staining (magnification ×2, scale bar = 1 mm) and safranin O staining (magnification ×2, scale bar = 1 mm) – (a) at four weeks after operation, and (b) at eight weeks after operation.
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Figure 4: Representative histological appearance of the mid-coronal proximal left tibia. H&E staining (magnification ×2, scale bar = 1 mm) and safranin O staining (magnification ×2, scale bar = 1 mm) – (a) at four weeks after operation, and (b) at eight weeks after operation.

Mentions: Histological findings in the operated control group showed that the bony bridge was formed between the epiphysis and metaphysis at four and eight weeks post-operatively with H&E staining (Fig. 4). The mean bony bridge formation rate was 78.5% (50% to 100%) at four weeks, and 86% (60% to 100%) at eight weeks, post-operatively. Safranin O staining showed the growth plate was discontinuous at the operated site.


Costal cartilage transplantation for treatment of growth plate injury in a rabbit model
Representative histological appearance of the mid-coronal proximal left tibia. H&E staining (magnification ×2, scale bar = 1 mm) and safranin O staining (magnification ×2, scale bar = 1 mm) – (a) at four weeks after operation, and (b) at eight weeks after operation.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC5382332&req=5

Figure 4: Representative histological appearance of the mid-coronal proximal left tibia. H&E staining (magnification ×2, scale bar = 1 mm) and safranin O staining (magnification ×2, scale bar = 1 mm) – (a) at four weeks after operation, and (b) at eight weeks after operation.
Mentions: Histological findings in the operated control group showed that the bony bridge was formed between the epiphysis and metaphysis at four and eight weeks post-operatively with H&E staining (Fig. 4). The mean bony bridge formation rate was 78.5% (50% to 100%) at four weeks, and 86% (60% to 100%) at eight weeks, post-operatively. Safranin O staining showed the growth plate was discontinuous at the operated site.

View Article: PubMed Central - PubMed

ABSTRACT

Objectives: The purpose of this study is to evaluate the effects of allogenic costal cartilage transplantation on preventing bony bridge formation and angular deformities for the treatment of partial growth plate injury using a rabbit model.

Methods: An experimental model of partial growth injury was created by resecting the medial part of the proximal tibial growth plate in male six-week-old New Zealand White rabbits. The rabbits were divided into four groups: no surgery; no transplantation; bone wax transplantation; and allogenic costal cartilage transplantation. The angular deformities of the tibia and bony bridge were analysed using radiographs and microcomputed tomography, and the repair of the injured growth plate cartilage and bony bridge formation rate were histologically evaluated.

Results: On radiographic evaluation, the varus deformities in the costal cartilage group were significantly improved compared with the no transplantation group at four and eight weeks after operation and with the bone wax group at eight weeks after operation. Micro-computed tomography showed that the bony bridge formation was prevented in the bone wax and costal cartilage groups. Histological findings showed that the bony bridge formation in the bone wax and costal cartilage group was decreased. In addition, the growth plate was continuous and stained with safranin O and immunohistochemically stained for type II collagen.

Conclusion: Transplantation of costal cartilage improved angular deformities and decreased bony bridge formation in the partial growth plate injury. Costal cartilage might be a suitable graft for the treatment of growth plate injury.

No MeSH data available.