Limits...
Costal cartilage transplantation for treatment of growth plate injury in a rabbit model

View Article: PubMed Central - PubMed

ABSTRACT

Objectives: The purpose of this study is to evaluate the effects of allogenic costal cartilage transplantation on preventing bony bridge formation and angular deformities for the treatment of partial growth plate injury using a rabbit model.

Methods: An experimental model of partial growth injury was created by resecting the medial part of the proximal tibial growth plate in male six-week-old New Zealand White rabbits. The rabbits were divided into four groups: no surgery; no transplantation; bone wax transplantation; and allogenic costal cartilage transplantation. The angular deformities of the tibia and bony bridge were analysed using radiographs and microcomputed tomography, and the repair of the injured growth plate cartilage and bony bridge formation rate were histologically evaluated.

Results: On radiographic evaluation, the varus deformities in the costal cartilage group were significantly improved compared with the no transplantation group at four and eight weeks after operation and with the bone wax group at eight weeks after operation. Micro-computed tomography showed that the bony bridge formation was prevented in the bone wax and costal cartilage groups. Histological findings showed that the bony bridge formation in the bone wax and costal cartilage group was decreased. In addition, the growth plate was continuous and stained with safranin O and immunohistochemically stained for type II collagen.

Conclusion: Transplantation of costal cartilage improved angular deformities and decreased bony bridge formation in the partial growth plate injury. Costal cartilage might be a suitable graft for the treatment of growth plate injury.

No MeSH data available.


Schematic showing the surgical procedure. The medial half of the growth plate of the left tibia was drilled from the inside using a high-speed steel bur, and injured in the range of 3mm diameter and 5mm depth. Tailored costal cartilage was transplanted to the excavated space.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
getmorefigures.php?uid=PMC5382332&req=5

Figure 1: Schematic showing the surgical procedure. The medial half of the growth plate of the left tibia was drilled from the inside using a high-speed steel bur, and injured in the range of 3mm diameter and 5mm depth. Tailored costal cartilage was transplanted to the excavated space.

Mentions: The operation was performed as previously reported.13 Briefly, a skin incision was made at the medial proximal left tibia. The medial half of the growth plate of the tibia was drilled from the inside using a high-speed steel bur and injured in the range of 3mm diameter and 5mm depth. The excavated space created by drilling was filled with either bone wax or costal cartilage graft, which was tailored to the shape of the space (Fig. 1). Nothing was transplanted in the operated control group. After the operation, the rabbits received 1.5 mg/kg of carprofen intravenously for post-operative analgesia, but did not have any load or mobility restrictions.


Costal cartilage transplantation for treatment of growth plate injury in a rabbit model
Schematic showing the surgical procedure. The medial half of the growth plate of the left tibia was drilled from the inside using a high-speed steel bur, and injured in the range of 3mm diameter and 5mm depth. Tailored costal cartilage was transplanted to the excavated space.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC5382332&req=5

Figure 1: Schematic showing the surgical procedure. The medial half of the growth plate of the left tibia was drilled from the inside using a high-speed steel bur, and injured in the range of 3mm diameter and 5mm depth. Tailored costal cartilage was transplanted to the excavated space.
Mentions: The operation was performed as previously reported.13 Briefly, a skin incision was made at the medial proximal left tibia. The medial half of the growth plate of the tibia was drilled from the inside using a high-speed steel bur and injured in the range of 3mm diameter and 5mm depth. The excavated space created by drilling was filled with either bone wax or costal cartilage graft, which was tailored to the shape of the space (Fig. 1). Nothing was transplanted in the operated control group. After the operation, the rabbits received 1.5 mg/kg of carprofen intravenously for post-operative analgesia, but did not have any load or mobility restrictions.

View Article: PubMed Central - PubMed

ABSTRACT

Objectives: The purpose of this study is to evaluate the effects of allogenic costal cartilage transplantation on preventing bony bridge formation and angular deformities for the treatment of partial growth plate injury using a rabbit model.

Methods: An experimental model of partial growth injury was created by resecting the medial part of the proximal tibial growth plate in male six-week-old New Zealand White rabbits. The rabbits were divided into four groups: no surgery; no transplantation; bone wax transplantation; and allogenic costal cartilage transplantation. The angular deformities of the tibia and bony bridge were analysed using radiographs and microcomputed tomography, and the repair of the injured growth plate cartilage and bony bridge formation rate were histologically evaluated.

Results: On radiographic evaluation, the varus deformities in the costal cartilage group were significantly improved compared with the no transplantation group at four and eight weeks after operation and with the bone wax group at eight weeks after operation. Micro-computed tomography showed that the bony bridge formation was prevented in the bone wax and costal cartilage groups. Histological findings showed that the bony bridge formation in the bone wax and costal cartilage group was decreased. In addition, the growth plate was continuous and stained with safranin O and immunohistochemically stained for type II collagen.

Conclusion: Transplantation of costal cartilage improved angular deformities and decreased bony bridge formation in the partial growth plate injury. Costal cartilage might be a suitable graft for the treatment of growth plate injury.

No MeSH data available.