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Frequency and phenotype of natural killer cells and natural killer cell subsets in bovine lymphoid compartments and blood

View Article: PubMed Central - PubMed

ABSTRACT

Natural killer (NK) cells are widely distributed in lymphoid and non‐lymphoid tissues, but little is known about the recirculation of NK cells between blood and tissues. This is relevant to understanding recirculation in the steady‐state and also for determining the roles for NK cells in vaccine‐induced immunity and responses to infection. Therefore, the percentage of NK cells and their phenotype across peripheral blood, afferent lymph and lymph nodes in steady‐state conditions was investigated in cattle using the pseudo‐afferent lymphatic cannulation model. CD2+ CD25lo NK cells were the predominant subset of NK cells within the blood. In contrast, CD2− CD25hi NK cells were the main subset present within the skin‐draining afferent lymphatic vessels and lymph nodes, indicating that CD2− NK cells are the principal NK cell subset trafficking to lymph nodes via the afferent lymphatic vessel. Furthermore, a low percentage of NK cells were present in efferent lymph, which were predominantly of the CD2− subset, indicating that NK cells can egress from lymph nodes and return to circulation in steady‐state conditions. These compartmentalization data indicate that NK cells represent a population of recirculating lymphocytes in steady‐state conditions and therefore may be important during immune responses to vaccination or infection.

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CD2− natural killer (NK) cells are primed in efferent lymph. Lymphocytes derived from peripheral blood (PB), afferent lymph (AL), lymph nodes (LNs) and efferent lymph (EL) were labelled with monoclonal antibodies to NKp46, CD2 and CD25 and analysed by flow cytometry. Pooled data from seven calves for PB, AL and LNs, and five calves for EL, illustrate the average percentage of CD25+ NK cells ± SD within the total NKp46+ population from PB (circles), AL (squares), LNs (triangles) and EL (diamonds) (a). Pooled data from seven (PB, AL and LNs) and five (EL) calves shows the average percentage of NKp46+ CD2+ (lighter bars) and NKp46+ CD2− (darker bars) NK cells ± SD within the total gated NKp46+ CD25+ NK cell population from PB, AL, LN and EL (b). Data were normally distributed (P > 0·05) and significance between PB and EL was assessed using a two‐sample t‐test. P < 0·05*, P < 0·01**, P < 0·001***.
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imm12708-fig-0006: CD2− natural killer (NK) cells are primed in efferent lymph. Lymphocytes derived from peripheral blood (PB), afferent lymph (AL), lymph nodes (LNs) and efferent lymph (EL) were labelled with monoclonal antibodies to NKp46, CD2 and CD25 and analysed by flow cytometry. Pooled data from seven calves for PB, AL and LNs, and five calves for EL, illustrate the average percentage of CD25+ NK cells ± SD within the total NKp46+ population from PB (circles), AL (squares), LNs (triangles) and EL (diamonds) (a). Pooled data from seven (PB, AL and LNs) and five (EL) calves shows the average percentage of NKp46+ CD2+ (lighter bars) and NKp46+ CD2− (darker bars) NK cells ± SD within the total gated NKp46+ CD25+ NK cell population from PB, AL, LN and EL (b). Data were normally distributed (P > 0·05) and significance between PB and EL was assessed using a two‐sample t‐test. P < 0·05*, P < 0·01**, P < 0·001***.

Mentions: The expression of CD25 by EL‐derived NK cells was compared with NK cells from PB, AL and LNs. Of the NK cells from EL, 71·2% (66·8–76·6%; SD = 3·4) were CD25+ and, similar to those present in AL and LNs, the percentage of CD25+ NK cells was significantly increased in EL (P < 0·001) compared with PB (Fig. 6a). Furthermore, when assessing the subset of NK cells responsible for this enhanced activation of NK cells in the EL, 63·3% (45·1–77·3%; SD = 12·5) of CD25+ NK cells were CD2−; however, this was not significantly (P = 0·069) different to the percentage of CD2− CD25+ NK cells present in PB, AL or LNs (Fig. 6b).


Frequency and phenotype of natural killer cells and natural killer cell subsets in bovine lymphoid compartments and blood
CD2− natural killer (NK) cells are primed in efferent lymph. Lymphocytes derived from peripheral blood (PB), afferent lymph (AL), lymph nodes (LNs) and efferent lymph (EL) were labelled with monoclonal antibodies to NKp46, CD2 and CD25 and analysed by flow cytometry. Pooled data from seven calves for PB, AL and LNs, and five calves for EL, illustrate the average percentage of CD25+ NK cells ± SD within the total NKp46+ population from PB (circles), AL (squares), LNs (triangles) and EL (diamonds) (a). Pooled data from seven (PB, AL and LNs) and five (EL) calves shows the average percentage of NKp46+ CD2+ (lighter bars) and NKp46+ CD2− (darker bars) NK cells ± SD within the total gated NKp46+ CD25+ NK cell population from PB, AL, LN and EL (b). Data were normally distributed (P > 0·05) and significance between PB and EL was assessed using a two‐sample t‐test. P < 0·05*, P < 0·01**, P < 0·001***.
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Related In: Results  -  Collection

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getmorefigures.php?uid=PMC5382329&req=5

imm12708-fig-0006: CD2− natural killer (NK) cells are primed in efferent lymph. Lymphocytes derived from peripheral blood (PB), afferent lymph (AL), lymph nodes (LNs) and efferent lymph (EL) were labelled with monoclonal antibodies to NKp46, CD2 and CD25 and analysed by flow cytometry. Pooled data from seven calves for PB, AL and LNs, and five calves for EL, illustrate the average percentage of CD25+ NK cells ± SD within the total NKp46+ population from PB (circles), AL (squares), LNs (triangles) and EL (diamonds) (a). Pooled data from seven (PB, AL and LNs) and five (EL) calves shows the average percentage of NKp46+ CD2+ (lighter bars) and NKp46+ CD2− (darker bars) NK cells ± SD within the total gated NKp46+ CD25+ NK cell population from PB, AL, LN and EL (b). Data were normally distributed (P > 0·05) and significance between PB and EL was assessed using a two‐sample t‐test. P < 0·05*, P < 0·01**, P < 0·001***.
Mentions: The expression of CD25 by EL‐derived NK cells was compared with NK cells from PB, AL and LNs. Of the NK cells from EL, 71·2% (66·8–76·6%; SD = 3·4) were CD25+ and, similar to those present in AL and LNs, the percentage of CD25+ NK cells was significantly increased in EL (P < 0·001) compared with PB (Fig. 6a). Furthermore, when assessing the subset of NK cells responsible for this enhanced activation of NK cells in the EL, 63·3% (45·1–77·3%; SD = 12·5) of CD25+ NK cells were CD2−; however, this was not significantly (P = 0·069) different to the percentage of CD2− CD25+ NK cells present in PB, AL or LNs (Fig. 6b).

View Article: PubMed Central - PubMed

ABSTRACT

Natural killer (NK) cells are widely distributed in lymphoid and non&#8208;lymphoid tissues, but little is known about the recirculation of NK cells between blood and tissues. This is relevant to understanding recirculation in the steady&#8208;state and also for determining the roles for NK cells in vaccine&#8208;induced immunity and responses to infection. Therefore, the percentage of NK cells and their phenotype across peripheral blood, afferent lymph and lymph nodes in steady&#8208;state conditions was investigated in cattle using the pseudo&#8208;afferent lymphatic cannulation model. CD2+ CD25lo NK cells were the predominant subset of NK cells within the blood. In contrast, CD2&minus; CD25hi NK cells were the main subset present within the skin&#8208;draining afferent lymphatic vessels and lymph nodes, indicating that CD2&minus; NK cells are the principal NK cell subset trafficking to lymph nodes via the afferent lymphatic vessel. Furthermore, a low percentage of NK cells were present in efferent lymph, which were predominantly of the CD2&minus; subset, indicating that NK cells can egress from lymph nodes and return to circulation in steady&#8208;state conditions. These compartmentalization data indicate that NK cells represent a population of recirculating lymphocytes in steady&#8208;state conditions and therefore may be important during immune responses to vaccination or infection.

No MeSH data available.


Related in: MedlinePlus