Limits...
Distinguishing the Unique Neuropathological Profile of Blast Polytrauma

View Article: PubMed Central - PubMed

ABSTRACT

Traumatic brain injury sustained after blast exposure (blast-induced TBI) has recently been documented as a growing issue for military personnel. Incidence of injury to organs such as the lungs has decreased, though current epidemiology still causes a great public health burden. In addition, unprotected civilians sustain primary blast lung injury (PBLI) at alarming rates. Often, mild-to-moderate cases of PBLI are survivable with medical intervention, which creates a growing population of survivors of blast-induced polytrauma (BPT) with symptoms from blast-induced mild TBI (mTBI). Currently, there is a lack of preclinical models simulating BPT, which is crucial to identifying unique injury mechanisms of BPT and its management. To meet this need, our group characterized a rodent model of BPT and compared results to a blast-induced mTBI model. Open field (OF) performance trials were performed on rodents at 7 days after injury. Immunohistochemistry was performed to evaluate cellular outcome at day seven following BPT. Levels of reactive astrocytes (GFAP), apoptosis (cleaved caspase-3 expression), and vascular damage (SMI-71) were significantly elevated in BPT compared to blast-induced mTBI. Downstream markers of hypoxia (HIF-1α and VEGF) were higher only after BPT. This study highlights the need for unique therapeutics and prehospital management when handling BPT.

No MeSH data available.


Fraction of time spent at the walls of the open arena was significantly higher in the mTBI and BPT groups compared to sham at 7 days after blast (∗p < 0.02, #p < 0.05). Representative images show animal tracking over five minutes.
© Copyright Policy
Related In: Results  -  Collection

License
getmorefigures.php?uid=PMC5382305&req=5

fig1: Fraction of time spent at the walls of the open arena was significantly higher in the mTBI and BPT groups compared to sham at 7 days after blast (∗p < 0.02, #p < 0.05). Representative images show animal tracking over five minutes.

Mentions: The fraction of time spent at the walls of the open field box for the BPT group was significantly increased (p < 0.02) compared to sham (Figure 1). The mTBI group also displayed elevated anxiety (p < 0.05) compared to sham. Representative image of animal activity over the five-minute period in the open arena demonstrates global exploration by the sham group and proximity to the walls in the BPT group (Figure 1). This display of anxiety-like behavior in the BPT group could be the neurological manifestation of injury pathology.


Distinguishing the Unique Neuropathological Profile of Blast Polytrauma
Fraction of time spent at the walls of the open arena was significantly higher in the mTBI and BPT groups compared to sham at 7 days after blast (∗p < 0.02, #p < 0.05). Representative images show animal tracking over five minutes.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC5382305&req=5

fig1: Fraction of time spent at the walls of the open arena was significantly higher in the mTBI and BPT groups compared to sham at 7 days after blast (∗p < 0.02, #p < 0.05). Representative images show animal tracking over five minutes.
Mentions: The fraction of time spent at the walls of the open field box for the BPT group was significantly increased (p < 0.02) compared to sham (Figure 1). The mTBI group also displayed elevated anxiety (p < 0.05) compared to sham. Representative image of animal activity over the five-minute period in the open arena demonstrates global exploration by the sham group and proximity to the walls in the BPT group (Figure 1). This display of anxiety-like behavior in the BPT group could be the neurological manifestation of injury pathology.

View Article: PubMed Central - PubMed

ABSTRACT

Traumatic brain injury sustained after blast exposure (blast-induced TBI) has recently been documented as a growing issue for military personnel. Incidence of injury to organs such as the lungs has decreased, though current epidemiology still causes a great public health burden. In addition, unprotected civilians sustain primary blast lung injury (PBLI) at alarming rates. Often, mild-to-moderate cases of PBLI are survivable with medical intervention, which creates a growing population of survivors of blast-induced polytrauma (BPT) with symptoms from blast-induced mild TBI (mTBI). Currently, there is a lack of preclinical models simulating BPT, which is crucial to identifying unique injury mechanisms of BPT and its management. To meet this need, our group characterized a rodent model of BPT and compared results to a blast-induced mTBI model. Open field (OF) performance trials were performed on rodents at 7 days after injury. Immunohistochemistry was performed to evaluate cellular outcome at day seven following BPT. Levels of reactive astrocytes (GFAP), apoptosis (cleaved caspase-3 expression), and vascular damage (SMI-71) were significantly elevated in BPT compared to blast-induced mTBI. Downstream markers of hypoxia (HIF-1&alpha; and VEGF) were higher only after BPT. This study highlights the need for unique therapeutics and prehospital management when handling BPT.

No MeSH data available.