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Design and Elementary Evaluation of a Highly-Automated Fluorescence-Based Instrument System for On-Site Detection of Food-Borne Pathogens

View Article: PubMed Central - PubMed

ABSTRACT

A simple, highly-automated instrument system used for on-site detection of foodborne pathogens based on fluorescence was designed, fabricated, and preliminarily tested in this paper. A corresponding method has been proved effective in our previous studies. This system utilizes a light-emitting diode (LED) to excite fluorescent labels and a spectrometer to record the fluorescence signal from samples. A rotation stage for positioning and switching samples was innovatively designed for high-throughput detection, ten at most in one single run. We also developed software based on LabVIEW for data receiving, processing, and the control of the whole system. In the test of using a pure quantum dot (QD) solution as a standard sample, detection results from this home-made system were highly-relevant with that from a well-commercialized product and even slightly better reproducibility was found. And in the test of three typical kinds of food-borne pathogens, fluorescence signals recorded by this system are highly proportional to the variation of the sample concentration, with a satisfied limit of detection (LOD) (nearly 102–103 CFU·mL−1 in food samples). Additionally, this instrument system is low-cost and easy-to-use, showing a promising potential for on-site rapid detection of food-borne pathogens.

No MeSH data available.


The linear relationship of E. coli O157:H7, L. monocytogenes, and S. Typhimurium at the concentration range from 100 to 107 CFU·mL−1 with the fluorescence intensity measured by this home-made instrument. The equations are ,  (E. coli O157:H7), ,  (L. monocytogenes), and ,  (S. Typhimurium), respectively.
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sensors-17-00442-f015: The linear relationship of E. coli O157:H7, L. monocytogenes, and S. Typhimurium at the concentration range from 100 to 107 CFU·mL−1 with the fluorescence intensity measured by this home-made instrument. The equations are , (E. coli O157:H7), , (L. monocytogenes), and , (S. Typhimurium), respectively.

Mentions: As introduced in Section 2.2, three typical kinds of food-borne bacteria, E. coli O157:H7, L. monocytogenes, and S. Typhimurium, were chosen to prove that this instrument system is able to detect bacteria. The test samples containing three kinds of bacteria together had been prepared from 100–107 CFU·mL−1. Three repeats for each sample were done and the linear relationships between the cell population (converted to log values) and the fluorescence intensity for each pathogen are presented in Figure 15.


Design and Elementary Evaluation of a Highly-Automated Fluorescence-Based Instrument System for On-Site Detection of Food-Borne Pathogens
The linear relationship of E. coli O157:H7, L. monocytogenes, and S. Typhimurium at the concentration range from 100 to 107 CFU·mL−1 with the fluorescence intensity measured by this home-made instrument. The equations are ,  (E. coli O157:H7), ,  (L. monocytogenes), and ,  (S. Typhimurium), respectively.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC5375728&req=5

sensors-17-00442-f015: The linear relationship of E. coli O157:H7, L. monocytogenes, and S. Typhimurium at the concentration range from 100 to 107 CFU·mL−1 with the fluorescence intensity measured by this home-made instrument. The equations are , (E. coli O157:H7), , (L. monocytogenes), and , (S. Typhimurium), respectively.
Mentions: As introduced in Section 2.2, three typical kinds of food-borne bacteria, E. coli O157:H7, L. monocytogenes, and S. Typhimurium, were chosen to prove that this instrument system is able to detect bacteria. The test samples containing three kinds of bacteria together had been prepared from 100–107 CFU·mL−1. Three repeats for each sample were done and the linear relationships between the cell population (converted to log values) and the fluorescence intensity for each pathogen are presented in Figure 15.

View Article: PubMed Central - PubMed

ABSTRACT

A simple, highly-automated instrument system used for on-site detection of foodborne pathogens based on fluorescence was designed, fabricated, and preliminarily tested in this paper. A corresponding method has been proved effective in our previous studies. This system utilizes a light-emitting diode (LED) to excite fluorescent labels and a spectrometer to record the fluorescence signal from samples. A rotation stage for positioning and switching samples was innovatively designed for high-throughput detection, ten at most in one single run. We also developed software based on LabVIEW for data receiving, processing, and the control of the whole system. In the test of using a pure quantum dot (QD) solution as a standard sample, detection results from this home-made system were highly-relevant with that from a well-commercialized product and even slightly better reproducibility was found. And in the test of three typical kinds of food-borne pathogens, fluorescence signals recorded by this system are highly proportional to the variation of the sample concentration, with a satisfied limit of detection (LOD) (nearly 102–103 CFU·mL−1 in food samples). Additionally, this instrument system is low-cost and easy-to-use, showing a promising potential for on-site rapid detection of food-borne pathogens.

No MeSH data available.