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Design and Elementary Evaluation of a Highly-Automated Fluorescence-Based Instrument System for On-Site Detection of Food-Borne Pathogens

View Article: PubMed Central - PubMed

ABSTRACT

A simple, highly-automated instrument system used for on-site detection of foodborne pathogens based on fluorescence was designed, fabricated, and preliminarily tested in this paper. A corresponding method has been proved effective in our previous studies. This system utilizes a light-emitting diode (LED) to excite fluorescent labels and a spectrometer to record the fluorescence signal from samples. A rotation stage for positioning and switching samples was innovatively designed for high-throughput detection, ten at most in one single run. We also developed software based on LabVIEW for data receiving, processing, and the control of the whole system. In the test of using a pure quantum dot (QD) solution as a standard sample, detection results from this home-made system were highly-relevant with that from a well-commercialized product and even slightly better reproducibility was found. And in the test of three typical kinds of food-borne pathogens, fluorescence signals recorded by this system are highly proportional to the variation of the sample concentration, with a satisfied limit of detection (LOD) (nearly 102–103 CFU·mL−1 in food samples). Additionally, this instrument system is low-cost and easy-to-use, showing a promising potential for on-site rapid detection of food-borne pathogens.

No MeSH data available.


(a) Comparison of the R.S.D. of the mean and median for the spectra data in a specific detection station. (b) Comparison of means and medians for each list of spectra data from ten detection stations.
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sensors-17-00442-f012: (a) Comparison of the R.S.D. of the mean and median for the spectra data in a specific detection station. (b) Comparison of means and medians for each list of spectra data from ten detection stations.

Mentions: For a quantitative comparison, means and medians of all of these lists of spectra data were calculated. As for each sample, measurements were repeated six times. The comparison of relative standard deviation (RSD) of these means and medians for each curve is represented in Figure 12a. Afterwards, the comparison in the form of fluorescence intensity of the means and the medians between these ten samples in ten different detection stations was also done and the result is shown in Figure 12b.


Design and Elementary Evaluation of a Highly-Automated Fluorescence-Based Instrument System for On-Site Detection of Food-Borne Pathogens
(a) Comparison of the R.S.D. of the mean and median for the spectra data in a specific detection station. (b) Comparison of means and medians for each list of spectra data from ten detection stations.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC5375728&req=5

sensors-17-00442-f012: (a) Comparison of the R.S.D. of the mean and median for the spectra data in a specific detection station. (b) Comparison of means and medians for each list of spectra data from ten detection stations.
Mentions: For a quantitative comparison, means and medians of all of these lists of spectra data were calculated. As for each sample, measurements were repeated six times. The comparison of relative standard deviation (RSD) of these means and medians for each curve is represented in Figure 12a. Afterwards, the comparison in the form of fluorescence intensity of the means and the medians between these ten samples in ten different detection stations was also done and the result is shown in Figure 12b.

View Article: PubMed Central - PubMed

ABSTRACT

A simple, highly-automated instrument system used for on-site detection of foodborne pathogens based on fluorescence was designed, fabricated, and preliminarily tested in this paper. A corresponding method has been proved effective in our previous studies. This system utilizes a light-emitting diode (LED) to excite fluorescent labels and a spectrometer to record the fluorescence signal from samples. A rotation stage for positioning and switching samples was innovatively designed for high-throughput detection, ten at most in one single run. We also developed software based on LabVIEW for data receiving, processing, and the control of the whole system. In the test of using a pure quantum dot (QD) solution as a standard sample, detection results from this home-made system were highly-relevant with that from a well-commercialized product and even slightly better reproducibility was found. And in the test of three typical kinds of food-borne pathogens, fluorescence signals recorded by this system are highly proportional to the variation of the sample concentration, with a satisfied limit of detection (LOD) (nearly 102–103 CFU·mL−1 in food samples). Additionally, this instrument system is low-cost and easy-to-use, showing a promising potential for on-site rapid detection of food-borne pathogens.

No MeSH data available.