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Design and Elementary Evaluation of a Highly-Automated Fluorescence-Based Instrument System for On-Site Detection of Food-Borne Pathogens

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ABSTRACT

A simple, highly-automated instrument system used for on-site detection of foodborne pathogens based on fluorescence was designed, fabricated, and preliminarily tested in this paper. A corresponding method has been proved effective in our previous studies. This system utilizes a light-emitting diode (LED) to excite fluorescent labels and a spectrometer to record the fluorescence signal from samples. A rotation stage for positioning and switching samples was innovatively designed for high-throughput detection, ten at most in one single run. We also developed software based on LabVIEW for data receiving, processing, and the control of the whole system. In the test of using a pure quantum dot (QD) solution as a standard sample, detection results from this home-made system were highly-relevant with that from a well-commercialized product and even slightly better reproducibility was found. And in the test of three typical kinds of food-borne pathogens, fluorescence signals recorded by this system are highly proportional to the variation of the sample concentration, with a satisfied limit of detection (LOD) (nearly 102–103 CFU·mL−1 in food samples). Additionally, this instrument system is low-cost and easy-to-use, showing a promising potential for on-site rapid detection of food-borne pathogens.

No MeSH data available.


Three spectra curves randomly selected from the test.
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sensors-17-00442-f011: Three spectra curves randomly selected from the test.

Mentions: Ten empty tubes and ten injectors with 0.1 mL of PBS were placed into the rotation stage, and the parameters related to data processing were settled as described in Section 4.2. To eliminate the interference of the light source whose center wavelength is near 460 nm, the range of the spectrum curve was limited to between 500 nm to 1040 nm. Three spectra curves selected randomly from the test are presented in Figure 11. The tendency and amplitude of them seemed to be quite approximate.


Design and Elementary Evaluation of a Highly-Automated Fluorescence-Based Instrument System for On-Site Detection of Food-Borne Pathogens
Three spectra curves randomly selected from the test.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC5375728&req=5

sensors-17-00442-f011: Three spectra curves randomly selected from the test.
Mentions: Ten empty tubes and ten injectors with 0.1 mL of PBS were placed into the rotation stage, and the parameters related to data processing were settled as described in Section 4.2. To eliminate the interference of the light source whose center wavelength is near 460 nm, the range of the spectrum curve was limited to between 500 nm to 1040 nm. Three spectra curves selected randomly from the test are presented in Figure 11. The tendency and amplitude of them seemed to be quite approximate.

View Article: PubMed Central - PubMed

ABSTRACT

A simple, highly-automated instrument system used for on-site detection of foodborne pathogens based on fluorescence was designed, fabricated, and preliminarily tested in this paper. A corresponding method has been proved effective in our previous studies. This system utilizes a light-emitting diode (LED) to excite fluorescent labels and a spectrometer to record the fluorescence signal from samples. A rotation stage for positioning and switching samples was innovatively designed for high-throughput detection, ten at most in one single run. We also developed software based on LabVIEW for data receiving, processing, and the control of the whole system. In the test of using a pure quantum dot (QD) solution as a standard sample, detection results from this home-made system were highly-relevant with that from a well-commercialized product and even slightly better reproducibility was found. And in the test of three typical kinds of food-borne pathogens, fluorescence signals recorded by this system are highly proportional to the variation of the sample concentration, with a satisfied limit of detection (LOD) (nearly 102–103 CFU·mL−1 in food samples). Additionally, this instrument system is low-cost and easy-to-use, showing a promising potential for on-site rapid detection of food-borne pathogens.

No MeSH data available.