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Design and Elementary Evaluation of a Highly-Automated Fluorescence-Based Instrument System for On-Site Detection of Food-Borne Pathogens

View Article: PubMed Central - PubMed

ABSTRACT

A simple, highly-automated instrument system used for on-site detection of foodborne pathogens based on fluorescence was designed, fabricated, and preliminarily tested in this paper. A corresponding method has been proved effective in our previous studies. This system utilizes a light-emitting diode (LED) to excite fluorescent labels and a spectrometer to record the fluorescence signal from samples. A rotation stage for positioning and switching samples was innovatively designed for high-throughput detection, ten at most in one single run. We also developed software based on LabVIEW for data receiving, processing, and the control of the whole system. In the test of using a pure quantum dot (QD) solution as a standard sample, detection results from this home-made system were highly-relevant with that from a well-commercialized product and even slightly better reproducibility was found. And in the test of three typical kinds of food-borne pathogens, fluorescence signals recorded by this system are highly proportional to the variation of the sample concentration, with a satisfied limit of detection (LOD) (nearly 102–103 CFU·mL−1 in food samples). Additionally, this instrument system is low-cost and easy-to-use, showing a promising potential for on-site rapid detection of food-borne pathogens.

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(a) Before and (b) after the use of the Savitzky-Golay filter and WA Multiscale Peak Detection. A green cross is used to mark the peak found by this algorithm.
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sensors-17-00442-f010: (a) Before and (b) after the use of the Savitzky-Golay filter and WA Multiscale Peak Detection. A green cross is used to mark the peak found by this algorithm.

Mentions: Other than the random error that could be reduced by proper average times, there was another type of error in the spectrum curve, as shown in Figure 10a, making the signal too coarse to read. A Savitzky-Golay filter was selected as the smoothing algorithm. In addition, a wavelet-based method for automatic peak-finding offered by NI, named WA Multiscale Peak Detection, was also added to search for the peak.


Design and Elementary Evaluation of a Highly-Automated Fluorescence-Based Instrument System for On-Site Detection of Food-Borne Pathogens
(a) Before and (b) after the use of the Savitzky-Golay filter and WA Multiscale Peak Detection. A green cross is used to mark the peak found by this algorithm.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC5375728&req=5

sensors-17-00442-f010: (a) Before and (b) after the use of the Savitzky-Golay filter and WA Multiscale Peak Detection. A green cross is used to mark the peak found by this algorithm.
Mentions: Other than the random error that could be reduced by proper average times, there was another type of error in the spectrum curve, as shown in Figure 10a, making the signal too coarse to read. A Savitzky-Golay filter was selected as the smoothing algorithm. In addition, a wavelet-based method for automatic peak-finding offered by NI, named WA Multiscale Peak Detection, was also added to search for the peak.

View Article: PubMed Central - PubMed

ABSTRACT

A simple, highly-automated instrument system used for on-site detection of foodborne pathogens based on fluorescence was designed, fabricated, and preliminarily tested in this paper. A corresponding method has been proved effective in our previous studies. This system utilizes a light-emitting diode (LED) to excite fluorescent labels and a spectrometer to record the fluorescence signal from samples. A rotation stage for positioning and switching samples was innovatively designed for high-throughput detection, ten at most in one single run. We also developed software based on LabVIEW for data receiving, processing, and the control of the whole system. In the test of using a pure quantum dot (QD) solution as a standard sample, detection results from this home-made system were highly-relevant with that from a well-commercialized product and even slightly better reproducibility was found. And in the test of three typical kinds of food-borne pathogens, fluorescence signals recorded by this system are highly proportional to the variation of the sample concentration, with a satisfied limit of detection (LOD) (nearly 102–103 CFU·mL−1 in food samples). Additionally, this instrument system is low-cost and easy-to-use, showing a promising potential for on-site rapid detection of food-borne pathogens.

No MeSH data available.


Related in: MedlinePlus