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Design and Elementary Evaluation of a Highly-Automated Fluorescence-Based Instrument System for On-Site Detection of Food-Borne Pathogens

View Article: PubMed Central - PubMed

ABSTRACT

A simple, highly-automated instrument system used for on-site detection of foodborne pathogens based on fluorescence was designed, fabricated, and preliminarily tested in this paper. A corresponding method has been proved effective in our previous studies. This system utilizes a light-emitting diode (LED) to excite fluorescent labels and a spectrometer to record the fluorescence signal from samples. A rotation stage for positioning and switching samples was innovatively designed for high-throughput detection, ten at most in one single run. We also developed software based on LabVIEW for data receiving, processing, and the control of the whole system. In the test of using a pure quantum dot (QD) solution as a standard sample, detection results from this home-made system were highly-relevant with that from a well-commercialized product and even slightly better reproducibility was found. And in the test of three typical kinds of food-borne pathogens, fluorescence signals recorded by this system are highly proportional to the variation of the sample concentration, with a satisfied limit of detection (LOD) (nearly 102–103 CFU·mL−1 in food samples). Additionally, this instrument system is low-cost and easy-to-use, showing a promising potential for on-site rapid detection of food-borne pathogens.

No MeSH data available.


(a) Overall arrangement of all of the components inside the shell. (b) The main user interface of this software program, where most assignments including spectrum collection, display, and system control are done.
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sensors-17-00442-f009: (a) Overall arrangement of all of the components inside the shell. (b) The main user interface of this software program, where most assignments including spectrum collection, display, and system control are done.

Mentions: Figure 9a presents the overall arrangement of all the components inside. The light source and the fiber optic spectrometer were fixed on the left wall of the instrument, connected together by the optical fiber probe. The whole mechanical unit was placed in the right side of the instrument. The power supply cables and USB communication lines went out from the instrument through a small hole on the bottom-rear of the unit. The hardware was covered by an aluminum extrusion-made shell, blocking out light from the environment. Figure 9b shows the main user interface of the LabVIEW-based software and this software had been installed in the notebook computer in advance.


Design and Elementary Evaluation of a Highly-Automated Fluorescence-Based Instrument System for On-Site Detection of Food-Borne Pathogens
(a) Overall arrangement of all of the components inside the shell. (b) The main user interface of this software program, where most assignments including spectrum collection, display, and system control are done.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC5375728&req=5

sensors-17-00442-f009: (a) Overall arrangement of all of the components inside the shell. (b) The main user interface of this software program, where most assignments including spectrum collection, display, and system control are done.
Mentions: Figure 9a presents the overall arrangement of all the components inside. The light source and the fiber optic spectrometer were fixed on the left wall of the instrument, connected together by the optical fiber probe. The whole mechanical unit was placed in the right side of the instrument. The power supply cables and USB communication lines went out from the instrument through a small hole on the bottom-rear of the unit. The hardware was covered by an aluminum extrusion-made shell, blocking out light from the environment. Figure 9b shows the main user interface of the LabVIEW-based software and this software had been installed in the notebook computer in advance.

View Article: PubMed Central - PubMed

ABSTRACT

A simple, highly-automated instrument system used for on-site detection of foodborne pathogens based on fluorescence was designed, fabricated, and preliminarily tested in this paper. A corresponding method has been proved effective in our previous studies. This system utilizes a light-emitting diode (LED) to excite fluorescent labels and a spectrometer to record the fluorescence signal from samples. A rotation stage for positioning and switching samples was innovatively designed for high-throughput detection, ten at most in one single run. We also developed software based on LabVIEW for data receiving, processing, and the control of the whole system. In the test of using a pure quantum dot (QD) solution as a standard sample, detection results from this home-made system were highly-relevant with that from a well-commercialized product and even slightly better reproducibility was found. And in the test of three typical kinds of food-borne pathogens, fluorescence signals recorded by this system are highly proportional to the variation of the sample concentration, with a satisfied limit of detection (LOD) (nearly 102–103 CFU·mL−1 in food samples). Additionally, this instrument system is low-cost and easy-to-use, showing a promising potential for on-site rapid detection of food-borne pathogens.

No MeSH data available.