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Design and Elementary Evaluation of a Highly-Automated Fluorescence-Based Instrument System for On-Site Detection of Food-Borne Pathogens

View Article: PubMed Central - PubMed

ABSTRACT

A simple, highly-automated instrument system used for on-site detection of foodborne pathogens based on fluorescence was designed, fabricated, and preliminarily tested in this paper. A corresponding method has been proved effective in our previous studies. This system utilizes a light-emitting diode (LED) to excite fluorescent labels and a spectrometer to record the fluorescence signal from samples. A rotation stage for positioning and switching samples was innovatively designed for high-throughput detection, ten at most in one single run. We also developed software based on LabVIEW for data receiving, processing, and the control of the whole system. In the test of using a pure quantum dot (QD) solution as a standard sample, detection results from this home-made system were highly-relevant with that from a well-commercialized product and even slightly better reproducibility was found. And in the test of three typical kinds of food-borne pathogens, fluorescence signals recorded by this system are highly proportional to the variation of the sample concentration, with a satisfied limit of detection (LOD) (nearly 102–103 CFU·mL−1 in food samples). Additionally, this instrument system is low-cost and easy-to-use, showing a promising potential for on-site rapid detection of food-borne pathogens.

No MeSH data available.


The typical flowchart of this software.
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sensors-17-00442-f008: The typical flowchart of this software.

Mentions: A typical flow chart of this software is illustrated in Figure 8. To start a detection, the operator would put in several single-use injectors that have already imbibed moderate samples and the same number of empty tubes, then set appropriate parameters and initialize the whole instrument system. After that, the system would work to obtain the spectral signals and then manage the result from them. At the end, when all of the samples in this run had been detected, the system would revert to its initial status. Detection results would be shown in the panel and saved on the computer automatically.


Design and Elementary Evaluation of a Highly-Automated Fluorescence-Based Instrument System for On-Site Detection of Food-Borne Pathogens
The typical flowchart of this software.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC5375728&req=5

sensors-17-00442-f008: The typical flowchart of this software.
Mentions: A typical flow chart of this software is illustrated in Figure 8. To start a detection, the operator would put in several single-use injectors that have already imbibed moderate samples and the same number of empty tubes, then set appropriate parameters and initialize the whole instrument system. After that, the system would work to obtain the spectral signals and then manage the result from them. At the end, when all of the samples in this run had been detected, the system would revert to its initial status. Detection results would be shown in the panel and saved on the computer automatically.

View Article: PubMed Central - PubMed

ABSTRACT

A simple, highly-automated instrument system used for on-site detection of foodborne pathogens based on fluorescence was designed, fabricated, and preliminarily tested in this paper. A corresponding method has been proved effective in our previous studies. This system utilizes a light-emitting diode (LED) to excite fluorescent labels and a spectrometer to record the fluorescence signal from samples. A rotation stage for positioning and switching samples was innovatively designed for high-throughput detection, ten at most in one single run. We also developed software based on LabVIEW for data receiving, processing, and the control of the whole system. In the test of using a pure quantum dot (QD) solution as a standard sample, detection results from this home-made system were highly-relevant with that from a well-commercialized product and even slightly better reproducibility was found. And in the test of three typical kinds of food-borne pathogens, fluorescence signals recorded by this system are highly proportional to the variation of the sample concentration, with a satisfied limit of detection (LOD) (nearly 102–103 CFU·mL−1 in food samples). Additionally, this instrument system is low-cost and easy-to-use, showing a promising potential for on-site rapid detection of food-borne pathogens.

No MeSH data available.