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Design and Elementary Evaluation of a Highly-Automated Fluorescence-Based Instrument System for On-Site Detection of Food-Borne Pathogens

View Article: PubMed Central - PubMed

ABSTRACT

A simple, highly-automated instrument system used for on-site detection of foodborne pathogens based on fluorescence was designed, fabricated, and preliminarily tested in this paper. A corresponding method has been proved effective in our previous studies. This system utilizes a light-emitting diode (LED) to excite fluorescent labels and a spectrometer to record the fluorescence signal from samples. A rotation stage for positioning and switching samples was innovatively designed for high-throughput detection, ten at most in one single run. We also developed software based on LabVIEW for data receiving, processing, and the control of the whole system. In the test of using a pure quantum dot (QD) solution as a standard sample, detection results from this home-made system were highly-relevant with that from a well-commercialized product and even slightly better reproducibility was found. And in the test of three typical kinds of food-borne pathogens, fluorescence signals recorded by this system are highly proportional to the variation of the sample concentration, with a satisfied limit of detection (LOD) (nearly 102–103 CFU·mL−1 in food samples). Additionally, this instrument system is low-cost and easy-to-use, showing a promising potential for on-site rapid detection of food-borne pathogens.

No MeSH data available.


Detailed configuration of this custom-built optical fiber.
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sensors-17-00442-f003: Detailed configuration of this custom-built optical fiber.

Mentions: The optical fiber probe was custom-built in a “Y” shape, as shown in Figure 2, two branches divided near the light source, one directly pointing to the sample and the other connecting with the fiber optic spectrometer. A metal roundel with two thread holes was customized to be fixed to the branch of the optical fiber probe pointing to the tube, so the fiber probe could be fixed with the optical-fiber-probe-fixed block by two hex bolts, as depicted in Figure 3, and now the position of the optical fiber could be controlled by the mechanical unit.


Design and Elementary Evaluation of a Highly-Automated Fluorescence-Based Instrument System for On-Site Detection of Food-Borne Pathogens
Detailed configuration of this custom-built optical fiber.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC5375728&req=5

sensors-17-00442-f003: Detailed configuration of this custom-built optical fiber.
Mentions: The optical fiber probe was custom-built in a “Y” shape, as shown in Figure 2, two branches divided near the light source, one directly pointing to the sample and the other connecting with the fiber optic spectrometer. A metal roundel with two thread holes was customized to be fixed to the branch of the optical fiber probe pointing to the tube, so the fiber probe could be fixed with the optical-fiber-probe-fixed block by two hex bolts, as depicted in Figure 3, and now the position of the optical fiber could be controlled by the mechanical unit.

View Article: PubMed Central - PubMed

ABSTRACT

A simple, highly-automated instrument system used for on-site detection of foodborne pathogens based on fluorescence was designed, fabricated, and preliminarily tested in this paper. A corresponding method has been proved effective in our previous studies. This system utilizes a light-emitting diode (LED) to excite fluorescent labels and a spectrometer to record the fluorescence signal from samples. A rotation stage for positioning and switching samples was innovatively designed for high-throughput detection, ten at most in one single run. We also developed software based on LabVIEW for data receiving, processing, and the control of the whole system. In the test of using a pure quantum dot (QD) solution as a standard sample, detection results from this home-made system were highly-relevant with that from a well-commercialized product and even slightly better reproducibility was found. And in the test of three typical kinds of food-borne pathogens, fluorescence signals recorded by this system are highly proportional to the variation of the sample concentration, with a satisfied limit of detection (LOD) (nearly 102–103 CFU·mL−1 in food samples). Additionally, this instrument system is low-cost and easy-to-use, showing a promising potential for on-site rapid detection of food-borne pathogens.

No MeSH data available.