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Spontaneous Deposition of Prussian Blue on Multi-Walled Carbon Nanotubes and the Application in an Amperometric Biosensor

View Article: PubMed Central - PubMed

ABSTRACT

A simple method has been developed for the spontaneous deposition of Prussian blue (PB) particles from a solution containing only ferricyanide ions onto conducting substrates such as indium tin oxide glass, glassy carbon disk and carbon nanotube (CNT) materials. Formation of PB deposits was confirmed by ultraviolet-visible absorption spectrometry and electrochemical techniques. The surface morphology of the PB particles deposited on the substrates was examined by atomic force microscopy and scanning electron microscopy. CNT/PB composite modified glassy carbon electrodes exhibited an electrocatalytic property for hydrogen peroxide reduction. These modified electrodes exhibited a high sensitivity for electrocatalytic reduction of hydrogen peroxide at −0.05 V (vs. Ag/AgCl), probably due to the synergistic effect of CNT with PB. Then, CNT/PB modified electrodes were further developed as amperometric glucose biosensors. These biosensors offered a linear response to glucose concentration from 0.1 to 0.9 mM with good selectivity, high sensitivity of 0.102 A M−1 cm−2 and short response time (within 2 s) at a negative operation potential of −0.05 V (vs. Ag/AgCl). The detection limit was estimated to be 0.01 mM at a signal-to-noise ratio of 3.

No MeSH data available.


Cyclic voltammograms of GC/CNT/PB modified electrode in phosphate buffer (pH 7.4) containing 5 mM phenylenediamine and 10 mg/mL glucose oxidase (GOD) at a scan rate of 50 mV/s.
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nanomaterials-02-00428-f006: Cyclic voltammograms of GC/CNT/PB modified electrode in phosphate buffer (pH 7.4) containing 5 mM phenylenediamine and 10 mg/mL glucose oxidase (GOD) at a scan rate of 50 mV/s.

Mentions: Glucose oxidase was immobilized on the GC/CNT/PB surface through electropolymerization of 5 mM phenylenediamine containing 10 mg/mL GOD between −0.30 and +0.70 V for 60 cycles in the present work. Cyclic voltammograms for co-immobilisation of PPD and GOD at the GC/CNT/PB electrode are shown in Figure 6. Oxidation of phenylenediamine was observed at potentials more positive than +0.60 V. The redox peaks at +0.15 and +0.35 V corresponded to the redox switching of the Prussian blue/Prussian white couple. After the first cycle, a sharp decrease in the oxidation current of phenylenediamine was observed, due to the presence of the nonconducting PPD film on the electrode surface. As the scanning number increased, the oxidation current reached a constant value, indicating the polymerization process was self-terminated [54,55]. Upon continuous voltammetric cycling, PPD film formed on the GC/CNT/PB modified surface. The peak current for PB deposits gradually decreased and reached constant values after 50 cycles. It indicated that the PB film was stable.


Spontaneous Deposition of Prussian Blue on Multi-Walled Carbon Nanotubes and the Application in an Amperometric Biosensor
Cyclic voltammograms of GC/CNT/PB modified electrode in phosphate buffer (pH 7.4) containing 5 mM phenylenediamine and 10 mg/mL glucose oxidase (GOD) at a scan rate of 50 mV/s.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC5304602&req=5

nanomaterials-02-00428-f006: Cyclic voltammograms of GC/CNT/PB modified electrode in phosphate buffer (pH 7.4) containing 5 mM phenylenediamine and 10 mg/mL glucose oxidase (GOD) at a scan rate of 50 mV/s.
Mentions: Glucose oxidase was immobilized on the GC/CNT/PB surface through electropolymerization of 5 mM phenylenediamine containing 10 mg/mL GOD between −0.30 and +0.70 V for 60 cycles in the present work. Cyclic voltammograms for co-immobilisation of PPD and GOD at the GC/CNT/PB electrode are shown in Figure 6. Oxidation of phenylenediamine was observed at potentials more positive than +0.60 V. The redox peaks at +0.15 and +0.35 V corresponded to the redox switching of the Prussian blue/Prussian white couple. After the first cycle, a sharp decrease in the oxidation current of phenylenediamine was observed, due to the presence of the nonconducting PPD film on the electrode surface. As the scanning number increased, the oxidation current reached a constant value, indicating the polymerization process was self-terminated [54,55]. Upon continuous voltammetric cycling, PPD film formed on the GC/CNT/PB modified surface. The peak current for PB deposits gradually decreased and reached constant values after 50 cycles. It indicated that the PB film was stable.

View Article: PubMed Central - PubMed

ABSTRACT

A simple method has been developed for the spontaneous deposition of Prussian blue (PB) particles from a solution containing only ferricyanide ions onto conducting substrates such as indium tin oxide glass, glassy carbon disk and carbon nanotube (CNT) materials. Formation of PB deposits was confirmed by ultraviolet-visible absorption spectrometry and electrochemical techniques. The surface morphology of the PB particles deposited on the substrates was examined by atomic force microscopy and scanning electron microscopy. CNT/PB composite modified glassy carbon electrodes exhibited an electrocatalytic property for hydrogen peroxide reduction. These modified electrodes exhibited a high sensitivity for electrocatalytic reduction of hydrogen peroxide at −0.05 V (vs. Ag/AgCl), probably due to the synergistic effect of CNT with PB. Then, CNT/PB modified electrodes were further developed as amperometric glucose biosensors. These biosensors offered a linear response to glucose concentration from 0.1 to 0.9 mM with good selectivity, high sensitivity of 0.102 A M−1 cm−2 and short response time (within 2 s) at a negative operation potential of −0.05 V (vs. Ag/AgCl). The detection limit was estimated to be 0.01 mM at a signal-to-noise ratio of 3.

No MeSH data available.