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Toxoplasma gondii Elongation Factor 1-Alpha (TgEF-1 α ) Is a Novel Vaccine Candidate Antigen against Toxoplasmosis

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ABSTRACT

Toxoplasma gondii (T. gondii) is an obligate intracellular parasite which can infect almost all warm-blood animals, leading to toxoplasmosis. Screening and discovery of an effective vaccine candidate or new drug target is crucial for the control of this disease. In this study, the recombinant T. gondii elongation factor 1-alpha (rTgEF-1α) was successfully expressed in in Escherichia coli. Passive immunization of mice with anti-rTgEF-1α polyclonal antibody following challenge with a lethal dose of tachyzoites significantly increased the survival time compared with PBS control group. The survival time of mice challenged with tachyzoites pretreated with anti-rTgEF-1α PcAb also was significantly increased. Invasion of tachyzoites into mouse macrophages was significantly inhibited in the anti-rTgEF-1α PcAb pretreated group. Mice vaccinated with rTgEF-1α induced a high level of specific anti-T. gondii antibodies and production of IFN-gamma, interleukin-4. The expression levels of MHC-I and MHC-II molecules as well as the percentages of CD4+ and CD8+ T cells in mice vaccinated with rTgEF-1α was significantly increased, respectively (P < 0.05), compared with all the controls. Immunization with rTgEF-1α significantly (P < 0.05) prolonged survival time (14.53 ± 1.72 days) after challenge infection with the virulent T. gondii RH strain. These results indicate that T. gondii EF-1α plays an essential role in mediating host cell invasion by the parasite and, as such, could be a candidate vaccine antigen against toxoplasmosis.

No MeSH data available.


Related in: MedlinePlus

The dynamics of cytokine production in BALB/c mice induced by rTgEF-1α vaccination. Antibody-captured ELISA was used to determine the production levels of (A) IFN-γ, (B) IL-4, (C) IL-17, and (D) TGF-β1, in sera samples (n = 5) collected at weeks 0, 2, 4, and 6, and the comparison results were expressed as means ± SD of pg/ml. The asterisk designates statistically significant differences (∗p < 0.05; ∗∗p < 0.01) between groups. Results presented here were from three independent experiments.
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Figure 4: The dynamics of cytokine production in BALB/c mice induced by rTgEF-1α vaccination. Antibody-captured ELISA was used to determine the production levels of (A) IFN-γ, (B) IL-4, (C) IL-17, and (D) TGF-β1, in sera samples (n = 5) collected at weeks 0, 2, 4, and 6, and the comparison results were expressed as means ± SD of pg/ml. The asterisk designates statistically significant differences (∗p < 0.05; ∗∗p < 0.01) between groups. Results presented here were from three independent experiments.

Mentions: Significantly higher levels of IFN-γ were produced in the sera of mice immunized with rTgEF-1α compared with the three control groups (P < 0.01, Figure 4A). On the other hand, low levels of IL-4 were detected in the groups of mice immunized with rTgEF-1α, which showed a slight but significant difference (P < 0.05) compared with mice immunized with the three control groups (Figure 4B). As for IL-17 and TGF-β1, both of them displayed no significant changes at similar times of evaluation (Figures 4C,D).


Toxoplasma gondii Elongation Factor 1-Alpha (TgEF-1 α ) Is a Novel Vaccine Candidate Antigen against Toxoplasmosis
The dynamics of cytokine production in BALB/c mice induced by rTgEF-1α vaccination. Antibody-captured ELISA was used to determine the production levels of (A) IFN-γ, (B) IL-4, (C) IL-17, and (D) TGF-β1, in sera samples (n = 5) collected at weeks 0, 2, 4, and 6, and the comparison results were expressed as means ± SD of pg/ml. The asterisk designates statistically significant differences (∗p < 0.05; ∗∗p < 0.01) between groups. Results presented here were from three independent experiments.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC5304420&req=5

Figure 4: The dynamics of cytokine production in BALB/c mice induced by rTgEF-1α vaccination. Antibody-captured ELISA was used to determine the production levels of (A) IFN-γ, (B) IL-4, (C) IL-17, and (D) TGF-β1, in sera samples (n = 5) collected at weeks 0, 2, 4, and 6, and the comparison results were expressed as means ± SD of pg/ml. The asterisk designates statistically significant differences (∗p < 0.05; ∗∗p < 0.01) between groups. Results presented here were from three independent experiments.
Mentions: Significantly higher levels of IFN-γ were produced in the sera of mice immunized with rTgEF-1α compared with the three control groups (P < 0.01, Figure 4A). On the other hand, low levels of IL-4 were detected in the groups of mice immunized with rTgEF-1α, which showed a slight but significant difference (P < 0.05) compared with mice immunized with the three control groups (Figure 4B). As for IL-17 and TGF-β1, both of them displayed no significant changes at similar times of evaluation (Figures 4C,D).

View Article: PubMed Central - PubMed

ABSTRACT

Toxoplasma gondii (T. gondii) is an obligate intracellular parasite which can infect almost all warm-blood animals, leading to toxoplasmosis. Screening and discovery of an effective vaccine candidate or new drug target is crucial for the control of this disease. In this study, the recombinant T. gondii elongation factor 1-alpha (rTgEF-1&alpha;) was successfully expressed in in Escherichia coli. Passive immunization of mice with anti-rTgEF-1&alpha; polyclonal antibody following challenge with a lethal dose of tachyzoites significantly increased the survival time compared with PBS control group. The survival time of mice challenged with tachyzoites pretreated with anti-rTgEF-1&alpha; PcAb also was significantly increased. Invasion of tachyzoites into mouse macrophages was significantly inhibited in the anti-rTgEF-1&alpha; PcAb pretreated group. Mice vaccinated with rTgEF-1&alpha; induced a high level of specific anti-T. gondii antibodies and production of IFN-gamma, interleukin-4. The expression levels of MHC-I and MHC-II molecules as well as the percentages of CD4+ and CD8+ T cells in mice vaccinated with rTgEF-1&alpha; was significantly increased, respectively (P &lt; 0.05), compared with all the controls. Immunization with rTgEF-1&alpha; significantly (P &lt; 0.05) prolonged survival time (14.53 &plusmn; 1.72 days) after challenge infection with the virulent T. gondii RH strain. These results indicate that T. gondii EF-1&alpha; plays an essential role in mediating host cell invasion by the parasite and, as such, could be a candidate vaccine antigen against toxoplasmosis.

No MeSH data available.


Related in: MedlinePlus