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Reelin and its receptors, VLDLR and ApoER2, in melanocytic nevi

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ABSTRACT

Reelin is an extracellular signaling protein synthesized by Cajal-Retius cells in utero and early after birth, its presence being signaled in adult life too. Reelin acts on its receptors, VLDLR and ApoER2, acting on cytoskeleton, controlling migration and subsequently positioning and stabilizing the cortical neurons.

We investigated the reelin presence and its receptors, VLDLR and ApoER2, in melanocytic nevi considering the neural crest origin of the nevus cells and their migration into skin during embrionary period.

Melanocytic nevi present a strict cellular architecture and an increased malignant transforming capacity. We investigated reelin presence in 32 melanocytic nevi (5 junctional, 27 compound or 14 dysplastic nevi and 18 non dysplastic nevi). The assessment of reelin presence was performed by histological semiquantitative criteria.

Results showed the presence of reelin in 29 cases (29/ 32). The presence of reelin was elevated in junctional areas as in dysplastic nevi. VLDLR presented positive values in 16 cases (16/ 32) and ApoER2 was weak positive in 7 cases. Reelin or its receptors was peritumorally absent. Our study showed the presence of reelin in nevus cells from cutaneous melanocytic nevi and, in these cells, only the VLDLR receptor was present in half of the cases.

The significance of the reelin presence in cutaneous nevus cells may be hypothetically considered correlated with the position maintenance of the nevus cells or migration of these cells in malignant transforming situation.

Abbreviations:: ApoER2 = apolipoprotein receptor 2, VLDLR = very low density lipoprotein receptor, DAB-1 = DIABLO protein, HMB45 = gene HMB45

No MeSH data available.


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e. Compound nevus with rare VLDLR positive cells (weak positive). Immunofluorescence with anti VLDLR antibodies (200X)
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Figure 5: e. Compound nevus with rare VLDLR positive cells (weak positive). Immunofluorescence with anti VLDLR antibodies (200X)

Mentions: Reelin was absent both in the dermis and epidermis in the peritumoral skin. VLDLR was shown in nevus melanocytes in 50% of the cases (16/ 32), with a weak staining intensity. All the junctional nevi stained weakly positive for VLDLR, as did 38% of the compound nevi (10/ 26). In dysplastic nevi, VLDLR stained weakly positive in 78% of the cases (11/ 14), and in 28% of the cases (5/ 18) of non-dysplastic nevi. Peritumorally, VLDLR stained weakly positive in 66% of the cases (8/ 12), while in 16% of the cases (5/ 32) VLDLR was weakly positive both within the tumor and peritumorally (Fig. 1 d,e). ApoER2 stained weakly positive in 22% of the cases (7/ 32), 3 of which were junctional nevi and 4 compound nevi, respectively, and 6 dysplastic nevi and 1 non-dysplastic nevus (Fig. 1f). ApoER2 was absent in the peritumoral skin. Biomarkers: S100, HMB45, and melanA were present in all the cases, while Ki67 showed values of less than 1% in 29 cases, 1-3% in 2 cases, and 12% in one case. The C-kit was significantly expressed in 17 cases (53%), 9 of which were dysplastic nevi and 8 non-dysplastic nevi. The intensely positive co-expression of reelin and c-kit was shown in 2 cases.


Reelin and its receptors, VLDLR and ApoER2, in melanocytic nevi
e. Compound nevus with rare VLDLR positive cells (weak positive). Immunofluorescence with anti VLDLR antibodies (200X)
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC5304381&req=5

Figure 5: e. Compound nevus with rare VLDLR positive cells (weak positive). Immunofluorescence with anti VLDLR antibodies (200X)
Mentions: Reelin was absent both in the dermis and epidermis in the peritumoral skin. VLDLR was shown in nevus melanocytes in 50% of the cases (16/ 32), with a weak staining intensity. All the junctional nevi stained weakly positive for VLDLR, as did 38% of the compound nevi (10/ 26). In dysplastic nevi, VLDLR stained weakly positive in 78% of the cases (11/ 14), and in 28% of the cases (5/ 18) of non-dysplastic nevi. Peritumorally, VLDLR stained weakly positive in 66% of the cases (8/ 12), while in 16% of the cases (5/ 32) VLDLR was weakly positive both within the tumor and peritumorally (Fig. 1 d,e). ApoER2 stained weakly positive in 22% of the cases (7/ 32), 3 of which were junctional nevi and 4 compound nevi, respectively, and 6 dysplastic nevi and 1 non-dysplastic nevus (Fig. 1f). ApoER2 was absent in the peritumoral skin. Biomarkers: S100, HMB45, and melanA were present in all the cases, while Ki67 showed values of less than 1% in 29 cases, 1-3% in 2 cases, and 12% in one case. The C-kit was significantly expressed in 17 cases (53%), 9 of which were dysplastic nevi and 8 non-dysplastic nevi. The intensely positive co-expression of reelin and c-kit was shown in 2 cases.

View Article: PubMed Central - PubMed

ABSTRACT

Reelin is an extracellular signaling protein synthesized by Cajal-Retius cells in utero and early after birth, its presence being signaled in adult life too. Reelin acts on its receptors, VLDLR and ApoER2, acting on cytoskeleton, controlling migration and subsequently positioning and stabilizing the cortical neurons.

We investigated the reelin presence and its receptors, VLDLR and ApoER2, in melanocytic nevi considering the neural crest origin of the nevus cells and their migration into skin during embrionary period.

Melanocytic nevi present a strict cellular architecture and an increased malignant transforming capacity. We investigated reelin presence in 32 melanocytic nevi (5 junctional, 27 compound or 14 dysplastic nevi and 18 non dysplastic nevi). The assessment of reelin presence was performed by histological semiquantitative criteria.

Results showed the presence of reelin in 29 cases (29/ 32). The presence of reelin was elevated in junctional areas as in dysplastic nevi. VLDLR presented positive values in 16 cases (16/ 32) and ApoER2 was weak positive in 7 cases. Reelin or its receptors was peritumorally absent. Our study showed the presence of reelin in nevus cells from cutaneous melanocytic nevi and, in these cells, only the VLDLR receptor was present in half of the cases.

The significance of the reelin presence in cutaneous nevus cells may be hypothetically considered correlated with the position maintenance of the nevus cells or migration of these cells in malignant transforming situation.

Abbreviations:: ApoER2 = apolipoprotein receptor 2, VLDLR = very low density lipoprotein receptor, DAB-1 = DIABLO protein, HMB45 = gene HMB45

No MeSH data available.


Related in: MedlinePlus