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Paenibacillus polymyxa NSY50 suppresses Fusarium wilt in cucumbers by regulating the rhizospheric microbial community

View Article: PubMed Central - PubMed

ABSTRACT

Paenibacillus polymyxa (P. polymyxa) NSY50, isolated from vinegar residue substrate, suppresses the growth of Fusarium oxysporum in the cucumber rhizosphere and protects the host plant from pathogen invasion. The aim of the present study was to evaluate the effects of NSY50 application on cucumber growth, soil properties and composition of the rhizospheric soil microbial community after exposure to Fusarium oxysporum. Bacterial and fungal communities were investigated by Illumina sequencing of the 16S rRNA gene and the internal transcribed spacer (ITS) regions (ITS1 and ITS2). The results showed that NSY50 effectively reduced the incidence of Fusarium wilt (56.4%) by altering the soil physico-chemical properties (e.g., pH, Cmic, Rmic, total N and Corg) and enzyme activities, especially of urease and β-glucosidase, which were significantly increased by 2.25- and 2.64-fold, respectively, relative to the pathogen treatment condition. More specifically, NSY50 application reduced the abundance of Fusarium and promoted potentially beneficial groups, including the Bacillus, Actinobacteria, Streptomyces, Actinospica, Catenulispora and Pseudomonas genera. Thus, our results suggest that NSY50 application can improve soil properties, shift the microbial community by increasing beneficial strains and decreasing pathogen colonization in the cucumber rhizosphere, and reduce the occurrence of cucumber Fusarium wilt, thereby promoting cucumber growth.

No MeSH data available.


The relative abundance of the main bacterial genera for the soil samples collected from the four treatment groups (CK, untreated plants (control); NSY50, plants challenged with NSY50 (2.5 × 108 CFU/mL); FOC, plants challenged with FOC (1 × 108 CFU/mL); NSY50 + FOC: plants challenged with NSY50 for 3 days and then with FOC).Each histogram represents the mean ± SE of three independent biological experiments (n = 3).
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f6: The relative abundance of the main bacterial genera for the soil samples collected from the four treatment groups (CK, untreated plants (control); NSY50, plants challenged with NSY50 (2.5 × 108 CFU/mL); FOC, plants challenged with FOC (1 × 108 CFU/mL); NSY50 + FOC: plants challenged with NSY50 for 3 days and then with FOC).Each histogram represents the mean ± SE of three independent biological experiments (n = 3).

Mentions: At the genus level (Figs 6 and 7), an analysis of the relative abundance of the top 80 classified bacterial genera and the top 20 classified fungal genera using a hierarchical heat map revealed a significant difference in the FOC and NSY50-challenged treatments. Amongst the top 80 classified bacterial genera (See Supplementary Fig. S3), many BCAs had been previously identified. We chose 6 BCAs as representatives (Fig. 6), including Bacillus, Actinobacteria, Streptomyces, Actinospica, Catenulispora, and Pseudomonas. Compared with the FOC treatment, NSY50 treatment significantly enriched the relative abundance of the BCAs. The relative abundance of the top 20 classified fungal genera (Fig. 7) was not sufficiently enriched in comparison with the bacterial genera. Interestingly, only Fusarium was represented as an abundant genus in the FOC and NSY50 + FOC conditions. Moreover, compared to the FOC treatment, NSY50 pretreatment (NSY50 + FOC) significantly reduced the abundance of Fusarium, which causes cucumber Fusarium wilt. However, the abundance of Penicillium was enriched in association with NSY50 (Fig. 7), because at the species level, the unclassified Penicillium occupied a large proportion in NSY50 (See Supplementary Table S2).


Paenibacillus polymyxa NSY50 suppresses Fusarium wilt in cucumbers by regulating the rhizospheric microbial community
The relative abundance of the main bacterial genera for the soil samples collected from the four treatment groups (CK, untreated plants (control); NSY50, plants challenged with NSY50 (2.5 × 108 CFU/mL); FOC, plants challenged with FOC (1 × 108 CFU/mL); NSY50 + FOC: plants challenged with NSY50 for 3 days and then with FOC).Each histogram represents the mean ± SE of three independent biological experiments (n = 3).
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC5304210&req=5

f6: The relative abundance of the main bacterial genera for the soil samples collected from the four treatment groups (CK, untreated plants (control); NSY50, plants challenged with NSY50 (2.5 × 108 CFU/mL); FOC, plants challenged with FOC (1 × 108 CFU/mL); NSY50 + FOC: plants challenged with NSY50 for 3 days and then with FOC).Each histogram represents the mean ± SE of three independent biological experiments (n = 3).
Mentions: At the genus level (Figs 6 and 7), an analysis of the relative abundance of the top 80 classified bacterial genera and the top 20 classified fungal genera using a hierarchical heat map revealed a significant difference in the FOC and NSY50-challenged treatments. Amongst the top 80 classified bacterial genera (See Supplementary Fig. S3), many BCAs had been previously identified. We chose 6 BCAs as representatives (Fig. 6), including Bacillus, Actinobacteria, Streptomyces, Actinospica, Catenulispora, and Pseudomonas. Compared with the FOC treatment, NSY50 treatment significantly enriched the relative abundance of the BCAs. The relative abundance of the top 20 classified fungal genera (Fig. 7) was not sufficiently enriched in comparison with the bacterial genera. Interestingly, only Fusarium was represented as an abundant genus in the FOC and NSY50 + FOC conditions. Moreover, compared to the FOC treatment, NSY50 pretreatment (NSY50 + FOC) significantly reduced the abundance of Fusarium, which causes cucumber Fusarium wilt. However, the abundance of Penicillium was enriched in association with NSY50 (Fig. 7), because at the species level, the unclassified Penicillium occupied a large proportion in NSY50 (See Supplementary Table S2).

View Article: PubMed Central - PubMed

ABSTRACT

Paenibacillus polymyxa (P. polymyxa) NSY50, isolated from vinegar residue substrate, suppresses the growth of Fusarium oxysporum in the cucumber rhizosphere and protects the host plant from pathogen invasion. The aim of the present study was to evaluate the effects of NSY50 application on cucumber growth, soil properties and composition of the rhizospheric soil microbial community after exposure to Fusarium oxysporum. Bacterial and fungal communities were investigated by Illumina sequencing of the 16S rRNA gene and the internal transcribed spacer (ITS) regions (ITS1 and ITS2). The results showed that NSY50 effectively reduced the incidence of Fusarium wilt (56.4%) by altering the soil physico-chemical properties (e.g., pH, Cmic, Rmic, total N and Corg) and enzyme activities, especially of urease and β-glucosidase, which were significantly increased by 2.25- and 2.64-fold, respectively, relative to the pathogen treatment condition. More specifically, NSY50 application reduced the abundance of Fusarium and promoted potentially beneficial groups, including the Bacillus, Actinobacteria, Streptomyces, Actinospica, Catenulispora and Pseudomonas genera. Thus, our results suggest that NSY50 application can improve soil properties, shift the microbial community by increasing beneficial strains and decreasing pathogen colonization in the cucumber rhizosphere, and reduce the occurrence of cucumber Fusarium wilt, thereby promoting cucumber growth.

No MeSH data available.