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Paenibacillus polymyxa NSY50 suppresses Fusarium wilt in cucumbers by regulating the rhizospheric microbial community

View Article: PubMed Central - PubMed

ABSTRACT

Paenibacillus polymyxa (P. polymyxa) NSY50, isolated from vinegar residue substrate, suppresses the growth of Fusarium oxysporum in the cucumber rhizosphere and protects the host plant from pathogen invasion. The aim of the present study was to evaluate the effects of NSY50 application on cucumber growth, soil properties and composition of the rhizospheric soil microbial community after exposure to Fusarium oxysporum. Bacterial and fungal communities were investigated by Illumina sequencing of the 16S rRNA gene and the internal transcribed spacer (ITS) regions (ITS1 and ITS2). The results showed that NSY50 effectively reduced the incidence of Fusarium wilt (56.4%) by altering the soil physico-chemical properties (e.g., pH, Cmic, Rmic, total N and Corg) and enzyme activities, especially of urease and β-glucosidase, which were significantly increased by 2.25- and 2.64-fold, respectively, relative to the pathogen treatment condition. More specifically, NSY50 application reduced the abundance of Fusarium and promoted potentially beneficial groups, including the Bacillus, Actinobacteria, Streptomyces, Actinospica, Catenulispora and Pseudomonas genera. Thus, our results suggest that NSY50 application can improve soil properties, shift the microbial community by increasing beneficial strains and decreasing pathogen colonization in the cucumber rhizosphere, and reduce the occurrence of cucumber Fusarium wilt, thereby promoting cucumber growth.

No MeSH data available.


Symptom development following the different treatments (CK, untreated plants (control); NSY50, plants challenged with NSY50 (2.5 × 108 CFU/mL); FOC, plants challenged with FOC (1 × 108 CFU/mL); NSY50 + FOC: plants challenged with NSY50 for 3 days and then with FOC) on cucumber growth on the 30th day after FOC inoculation.The pictures are representative of two independent biological experiments.
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f2: Symptom development following the different treatments (CK, untreated plants (control); NSY50, plants challenged with NSY50 (2.5 × 108 CFU/mL); FOC, plants challenged with FOC (1 × 108 CFU/mL); NSY50 + FOC: plants challenged with NSY50 for 3 days and then with FOC) on cucumber growth on the 30th day after FOC inoculation.The pictures are representative of two independent biological experiments.

Mentions: The effects of NSY50 inoculation on disease suppression and plant growth were investigated in cucumber plants (Fig. 1). After inoculating with the pathogen, the cucumber plants appeared to have disease symptoms, including higher levels of leaf yellowing and charcoal rot, with the FOC treatment (Fig. 2). The disease incidence reached 81.25% (Fig. 1a). However, pretreatment with NSY50 significantly reduced disease occurrence, with less yellowing present in the cucumber leaves. In addition, the plant height (Fig. 1b), fresh weight (Fig. 1c) and dry weight (Fig. 1d) all responded positively to the NSY50 treatment, and inoculation with NSY50 enhanced cucumber growth. Compared to the control treatment (CK), NSY50 treatment dramatically increased the plant height by 33.62%, the fresh weight by 36.45%, and the dry weight by 39.42%. Furthermore, the growth indices of cucumbers grown with NSY50 + FOC increased significantly in comparison with those grown with FOC alone.


Paenibacillus polymyxa NSY50 suppresses Fusarium wilt in cucumbers by regulating the rhizospheric microbial community
Symptom development following the different treatments (CK, untreated plants (control); NSY50, plants challenged with NSY50 (2.5 × 108 CFU/mL); FOC, plants challenged with FOC (1 × 108 CFU/mL); NSY50 + FOC: plants challenged with NSY50 for 3 days and then with FOC) on cucumber growth on the 30th day after FOC inoculation.The pictures are representative of two independent biological experiments.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC5304210&req=5

f2: Symptom development following the different treatments (CK, untreated plants (control); NSY50, plants challenged with NSY50 (2.5 × 108 CFU/mL); FOC, plants challenged with FOC (1 × 108 CFU/mL); NSY50 + FOC: plants challenged with NSY50 for 3 days and then with FOC) on cucumber growth on the 30th day after FOC inoculation.The pictures are representative of two independent biological experiments.
Mentions: The effects of NSY50 inoculation on disease suppression and plant growth were investigated in cucumber plants (Fig. 1). After inoculating with the pathogen, the cucumber plants appeared to have disease symptoms, including higher levels of leaf yellowing and charcoal rot, with the FOC treatment (Fig. 2). The disease incidence reached 81.25% (Fig. 1a). However, pretreatment with NSY50 significantly reduced disease occurrence, with less yellowing present in the cucumber leaves. In addition, the plant height (Fig. 1b), fresh weight (Fig. 1c) and dry weight (Fig. 1d) all responded positively to the NSY50 treatment, and inoculation with NSY50 enhanced cucumber growth. Compared to the control treatment (CK), NSY50 treatment dramatically increased the plant height by 33.62%, the fresh weight by 36.45%, and the dry weight by 39.42%. Furthermore, the growth indices of cucumbers grown with NSY50 + FOC increased significantly in comparison with those grown with FOC alone.

View Article: PubMed Central - PubMed

ABSTRACT

Paenibacillus polymyxa (P. polymyxa) NSY50, isolated from vinegar residue substrate, suppresses the growth of Fusarium oxysporum in the cucumber rhizosphere and protects the host plant from pathogen invasion. The aim of the present study was to evaluate the effects of NSY50 application on cucumber growth, soil properties and composition of the rhizospheric soil microbial community after exposure to Fusarium oxysporum. Bacterial and fungal communities were investigated by Illumina sequencing of the 16S rRNA gene and the internal transcribed spacer (ITS) regions (ITS1 and ITS2). The results showed that NSY50 effectively reduced the incidence of Fusarium wilt (56.4%) by altering the soil physico-chemical properties (e.g., pH, Cmic, Rmic, total N and Corg) and enzyme activities, especially of urease and β-glucosidase, which were significantly increased by 2.25- and 2.64-fold, respectively, relative to the pathogen treatment condition. More specifically, NSY50 application reduced the abundance of Fusarium and promoted potentially beneficial groups, including the Bacillus, Actinobacteria, Streptomyces, Actinospica, Catenulispora and Pseudomonas genera. Thus, our results suggest that NSY50 application can improve soil properties, shift the microbial community by increasing beneficial strains and decreasing pathogen colonization in the cucumber rhizosphere, and reduce the occurrence of cucumber Fusarium wilt, thereby promoting cucumber growth.

No MeSH data available.