Limits...
Three dimensional printing of calcium sulfate and mesoporous bioactive glass scaffolds for improving bone regeneration in vitro and in vivo

View Article: PubMed Central - PubMed

ABSTRACT

In the clinic, bone defects resulting from infections, trauma, surgical resection and genetic malformations remain a significant challenge. In the field of bone tissue engineering, three-dimensional (3D) scaffolds are promising for the treatment of bone defects. In this study, calcium sulfate hydrate (CSH)/mesoporous bioactive glass (MBG) scaffolds were successfully fabricated using a 3D printing technique, which had a regular and uniform square macroporous structure, high porosity and excellent apatite mineralization ability. Human bone marrow-derived mesenchymal stem cells (hBMSCs) were cultured on scaffolds to evaluate hBMSC attachment, proliferation and osteogenesis-related gene expression. Critical-sized rat calvarial defects were applied to investigate the effect of CSH/MBG scaffolds on bone regeneration in vivo. The in vitro results showed that CSH/MBG scaffolds stimulated the adhesion, proliferation, alkaline phosphatase (ALP) activity and osteogenesis-related gene expression of hBMSCs. In vivo results showed that CSH/MBG scaffolds could significantly enhance new bone formation in calvarial defects compared to CSH scaffolds. Thus 3D printed CSH/MBG scaffolds would be promising candidates for promoting bone regeneration.

No MeSH data available.


Related in: MedlinePlus

(A) Quantitative analysis of the proliferation of hBMSCs cultured on the CSH and CSH/MBG scaffolds shown for 1, 3 and 7 days (n = 3), (B) ALP activity of hBMSCs cultured for 7 and 14 days on the CSH and CSH/MBG scaffolds (*indicated significant differences when compared to CSH, P < 0.05).
© Copyright Policy - open-access
Related In: Results  -  Collection

License
getmorefigures.php?uid=PMC5304193&req=5

f8: (A) Quantitative analysis of the proliferation of hBMSCs cultured on the CSH and CSH/MBG scaffolds shown for 1, 3 and 7 days (n = 3), (B) ALP activity of hBMSCs cultured for 7 and 14 days on the CSH and CSH/MBG scaffolds (*indicated significant differences when compared to CSH, P < 0.05).

Mentions: The proliferation of hBMSCs cultured on the CSH, CSH/MBG20, CSH/MBG40 and CSH/MBG60 scaffolds for 3 and 7 days is shown in Fig. 8A. As determined by CCK-8 proliferation assay, the CSH, CSH/MBG20, CSH/MBG40 and CSH/MBG60 scaffolds all supported the proliferation of hBMSCs with increasing time in culture. However, the proliferation rates on the CSH/MBG20, CSH/MBG40 and CSH/MBG60 scaffolds were significantly higher than on the CSH scaffolds (P < 0.05). ALP activity of hBMSCs cultured on CSH/MBG scaffolds for 7 and 14 days are shown in Fig. 8B. Similar to the proliferation results, the CSH/MBG20, CSH/MBG40 and CSH/MBG60 scaffolds all exhibited significantly enhanced ALP activity compared to the CSH scaffolds (P < 0.05).


Three dimensional printing of calcium sulfate and mesoporous bioactive glass scaffolds for improving bone regeneration in vitro and in vivo
(A) Quantitative analysis of the proliferation of hBMSCs cultured on the CSH and CSH/MBG scaffolds shown for 1, 3 and 7 days (n = 3), (B) ALP activity of hBMSCs cultured for 7 and 14 days on the CSH and CSH/MBG scaffolds (*indicated significant differences when compared to CSH, P < 0.05).
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC5304193&req=5

f8: (A) Quantitative analysis of the proliferation of hBMSCs cultured on the CSH and CSH/MBG scaffolds shown for 1, 3 and 7 days (n = 3), (B) ALP activity of hBMSCs cultured for 7 and 14 days on the CSH and CSH/MBG scaffolds (*indicated significant differences when compared to CSH, P < 0.05).
Mentions: The proliferation of hBMSCs cultured on the CSH, CSH/MBG20, CSH/MBG40 and CSH/MBG60 scaffolds for 3 and 7 days is shown in Fig. 8A. As determined by CCK-8 proliferation assay, the CSH, CSH/MBG20, CSH/MBG40 and CSH/MBG60 scaffolds all supported the proliferation of hBMSCs with increasing time in culture. However, the proliferation rates on the CSH/MBG20, CSH/MBG40 and CSH/MBG60 scaffolds were significantly higher than on the CSH scaffolds (P < 0.05). ALP activity of hBMSCs cultured on CSH/MBG scaffolds for 7 and 14 days are shown in Fig. 8B. Similar to the proliferation results, the CSH/MBG20, CSH/MBG40 and CSH/MBG60 scaffolds all exhibited significantly enhanced ALP activity compared to the CSH scaffolds (P < 0.05).

View Article: PubMed Central - PubMed

ABSTRACT

In the clinic, bone defects resulting from infections, trauma, surgical resection and genetic malformations remain a significant challenge. In the field of bone tissue engineering, three-dimensional (3D) scaffolds are promising for the treatment of bone defects. In this study, calcium sulfate hydrate (CSH)/mesoporous bioactive glass (MBG) scaffolds were successfully fabricated using a 3D printing technique, which had a regular and uniform square macroporous structure, high porosity and excellent apatite mineralization ability. Human bone marrow-derived mesenchymal stem cells (hBMSCs) were cultured on scaffolds to evaluate hBMSC attachment, proliferation and osteogenesis-related gene expression. Critical-sized rat calvarial defects were applied to investigate the effect of CSH/MBG scaffolds on bone regeneration in vivo. The in vitro results showed that CSH/MBG scaffolds stimulated the adhesion, proliferation, alkaline phosphatase (ALP) activity and osteogenesis-related gene expression of hBMSCs. In vivo results showed that CSH/MBG scaffolds could significantly enhance new bone formation in calvarial defects compared to CSH scaffolds. Thus 3D printed CSH/MBG scaffolds would be promising candidates for promoting bone regeneration.

No MeSH data available.


Related in: MedlinePlus