Limits...
Three dimensional printing of calcium sulfate and mesoporous bioactive glass scaffolds for improving bone regeneration in vitro and in vivo

View Article: PubMed Central - PubMed

ABSTRACT

In the clinic, bone defects resulting from infections, trauma, surgical resection and genetic malformations remain a significant challenge. In the field of bone tissue engineering, three-dimensional (3D) scaffolds are promising for the treatment of bone defects. In this study, calcium sulfate hydrate (CSH)/mesoporous bioactive glass (MBG) scaffolds were successfully fabricated using a 3D printing technique, which had a regular and uniform square macroporous structure, high porosity and excellent apatite mineralization ability. Human bone marrow-derived mesenchymal stem cells (hBMSCs) were cultured on scaffolds to evaluate hBMSC attachment, proliferation and osteogenesis-related gene expression. Critical-sized rat calvarial defects were applied to investigate the effect of CSH/MBG scaffolds on bone regeneration in vivo. The in vitro results showed that CSH/MBG scaffolds stimulated the adhesion, proliferation, alkaline phosphatase (ALP) activity and osteogenesis-related gene expression of hBMSCs. In vivo results showed that CSH/MBG scaffolds could significantly enhance new bone formation in calvarial defects compared to CSH scaffolds. Thus 3D printed CSH/MBG scaffolds would be promising candidates for promoting bone regeneration.

No MeSH data available.


Related in: MedlinePlus

(A) Compressive strength of CSH and CSH/MBG scaffolds cured for various time periods (n = 3; *indicated significant differences compared to CSH, P < 0.05); (B) the compressive strength of CSH and CSH/MBG scaffolds after 7 days’ hydration and following 7 days’ immersion in SBF (n = 3; *indicated significant differences, P < 0.05).
© Copyright Policy - open-access
Related In: Results  -  Collection

License
getmorefigures.php?uid=PMC5304193&req=5

f4: (A) Compressive strength of CSH and CSH/MBG scaffolds cured for various time periods (n = 3; *indicated significant differences compared to CSH, P < 0.05); (B) the compressive strength of CSH and CSH/MBG scaffolds after 7 days’ hydration and following 7 days’ immersion in SBF (n = 3; *indicated significant differences, P < 0.05).

Mentions: The compressive strength of all 3D-printed scaffolds was measured after curing at 37 °C in 100% relative humidity for various time periods (Fig. 4A) and after SBF immersion for one week (Fig. 4B). With prolonging curing time, the compressive strengths of CSH, CSH/MBG20 and CSH/MBG40 scaffolds showed remarkable improvement. However, the compressive strength of CSH/MBG60 scaffolds exhibited no obvious change. After curing for 21 days, the compressive strengths of CSH, CSH/MBG20 CSH/MBG40, and CSH/MBG60 scaffolds were 12.8 ± 0.7 MPa, 10.4 ± 0.5 MPa, 8.2 ± 1.3 MPa and 4.5 ± 0.7 MPa, respectively. In addition, the compressive strength of scaffolds soaked in SBF for 7 days was slightly lower compared to those without SBF-soaked scaffolds (Fig. 4B).


Three dimensional printing of calcium sulfate and mesoporous bioactive glass scaffolds for improving bone regeneration in vitro and in vivo
(A) Compressive strength of CSH and CSH/MBG scaffolds cured for various time periods (n = 3; *indicated significant differences compared to CSH, P < 0.05); (B) the compressive strength of CSH and CSH/MBG scaffolds after 7 days’ hydration and following 7 days’ immersion in SBF (n = 3; *indicated significant differences, P < 0.05).
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC5304193&req=5

f4: (A) Compressive strength of CSH and CSH/MBG scaffolds cured for various time periods (n = 3; *indicated significant differences compared to CSH, P < 0.05); (B) the compressive strength of CSH and CSH/MBG scaffolds after 7 days’ hydration and following 7 days’ immersion in SBF (n = 3; *indicated significant differences, P < 0.05).
Mentions: The compressive strength of all 3D-printed scaffolds was measured after curing at 37 °C in 100% relative humidity for various time periods (Fig. 4A) and after SBF immersion for one week (Fig. 4B). With prolonging curing time, the compressive strengths of CSH, CSH/MBG20 and CSH/MBG40 scaffolds showed remarkable improvement. However, the compressive strength of CSH/MBG60 scaffolds exhibited no obvious change. After curing for 21 days, the compressive strengths of CSH, CSH/MBG20 CSH/MBG40, and CSH/MBG60 scaffolds were 12.8 ± 0.7 MPa, 10.4 ± 0.5 MPa, 8.2 ± 1.3 MPa and 4.5 ± 0.7 MPa, respectively. In addition, the compressive strength of scaffolds soaked in SBF for 7 days was slightly lower compared to those without SBF-soaked scaffolds (Fig. 4B).

View Article: PubMed Central - PubMed

ABSTRACT

In the clinic, bone defects resulting from infections, trauma, surgical resection and genetic malformations remain a significant challenge. In the field of bone tissue engineering, three-dimensional (3D) scaffolds are promising for the treatment of bone defects. In this study, calcium sulfate hydrate (CSH)/mesoporous bioactive glass (MBG) scaffolds were successfully fabricated using a 3D printing technique, which had a regular and uniform square macroporous structure, high porosity and excellent apatite mineralization ability. Human bone marrow-derived mesenchymal stem cells (hBMSCs) were cultured on scaffolds to evaluate hBMSC attachment, proliferation and osteogenesis-related gene expression. Critical-sized rat calvarial defects were applied to investigate the effect of CSH/MBG scaffolds on bone regeneration in vivo. The in vitro results showed that CSH/MBG scaffolds stimulated the adhesion, proliferation, alkaline phosphatase (ALP) activity and osteogenesis-related gene expression of hBMSCs. In vivo results showed that CSH/MBG scaffolds could significantly enhance new bone formation in calvarial defects compared to CSH scaffolds. Thus 3D printed CSH/MBG scaffolds would be promising candidates for promoting bone regeneration.

No MeSH data available.


Related in: MedlinePlus