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Changes in protein expression after treatment with Ancylostoma caninum excretory/secretory products in a mouse model of colitis

View Article: PubMed Central - PubMed

ABSTRACT

Different reports have highlighted the potential use of helminths and their secretions in the treatment of inflammatory bowel disease (IBD) conditions; however, no reports have investigated their effects at a proteome level. Herein, we characterise the protein expression changes that occur in lamina propria (LP) and the intestinal epithelial cells (IEC) of mice with dextran sulfate sodium (DSS)-induced colitis treated with Ancylostoma caninum excretory/secretory (ES) products using a quantitative proteomic approach. We have shown how parasite products can significantly alter the expression of proteins involved in immune responses, cell death and with an antioxidant activity. Interestingly, significant changes in the expression levels of different mucins were observed in this study. MUC13, a mucin implicated in gastrointestinal homeostasis, was upregulated in the LP of mice with DSS-induced colitis treated with ES, while MUC2, a major component of mucus, was upregulated in the IEC. In addition, A. caninum proteins have an important effect on proteins with antioxidant functions and proteins involved in intestinal homeostasis and tissue integrity and regeneration. Understanding how parasites can ameliorate IBD pathogenesis can help us design novel treatments for autoimmune diseases.

No MeSH data available.


Related in: MedlinePlus

Schematic overview of the experimental strategy followed.A total of three replicates (with six mice each) were analysed to study the effect of different Ancylostoma caninum excretory/secretory (ES) protein doses in the intestine of mice with DSS-induced colitis (A). The protein changes of the lamina propria (LP) and intestinal epithelial cell layer (IEC) of mice with DSS-induced colitis treated with A. caninum ES proteins were analysed in duplicate (B).
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f5: Schematic overview of the experimental strategy followed.A total of three replicates (with six mice each) were analysed to study the effect of different Ancylostoma caninum excretory/secretory (ES) protein doses in the intestine of mice with DSS-induced colitis (A). The protein changes of the lamina propria (LP) and intestinal epithelial cell layer (IEC) of mice with DSS-induced colitis treated with A. caninum ES proteins were analysed in duplicate (B).

Mentions: The DSS model of colitis has been previously described29. Colitis was induced by administering a 3.5% (wt/vol) solution of dextran sodium sulfate (DSS 36000–50000 kDa; MP Biomedicals) to mice as a substitute for normal drinking water. To determine the dose of ES that had the greatest ameliorating effect on protein expression changes observed during DSS-induced colitis, three mice (biological replicates) per experimental condition were analysed (Fig. 5). Four animals having DSS-induced colitis received daily intraperitoneal (i.p.) injections of different amounts of A. caninum ES (1, 5, 10 or 25 μg), and two groups of control animals (three mice with DSS-induced colitis and three healthy mice) received no ES treatment (Fig. 5). Mice were sacrificed and their intestine analysed at day 8 post DSS administration. For the proteomic analysis of the intestinal epithelial (IEC) layer and the lamina propria (LP) two replicates were performed using four groups of four mice with each group receiving either DSS only, ES only, DSS plus ES or neither DSS or ES (naïve group) (Fig. 5). The DSS only group received a mock injection of PBS instead of ES.


Changes in protein expression after treatment with Ancylostoma caninum excretory/secretory products in a mouse model of colitis
Schematic overview of the experimental strategy followed.A total of three replicates (with six mice each) were analysed to study the effect of different Ancylostoma caninum excretory/secretory (ES) protein doses in the intestine of mice with DSS-induced colitis (A). The protein changes of the lamina propria (LP) and intestinal epithelial cell layer (IEC) of mice with DSS-induced colitis treated with A. caninum ES proteins were analysed in duplicate (B).
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC5304188&req=5

f5: Schematic overview of the experimental strategy followed.A total of three replicates (with six mice each) were analysed to study the effect of different Ancylostoma caninum excretory/secretory (ES) protein doses in the intestine of mice with DSS-induced colitis (A). The protein changes of the lamina propria (LP) and intestinal epithelial cell layer (IEC) of mice with DSS-induced colitis treated with A. caninum ES proteins were analysed in duplicate (B).
Mentions: The DSS model of colitis has been previously described29. Colitis was induced by administering a 3.5% (wt/vol) solution of dextran sodium sulfate (DSS 36000–50000 kDa; MP Biomedicals) to mice as a substitute for normal drinking water. To determine the dose of ES that had the greatest ameliorating effect on protein expression changes observed during DSS-induced colitis, three mice (biological replicates) per experimental condition were analysed (Fig. 5). Four animals having DSS-induced colitis received daily intraperitoneal (i.p.) injections of different amounts of A. caninum ES (1, 5, 10 or 25 μg), and two groups of control animals (three mice with DSS-induced colitis and three healthy mice) received no ES treatment (Fig. 5). Mice were sacrificed and their intestine analysed at day 8 post DSS administration. For the proteomic analysis of the intestinal epithelial (IEC) layer and the lamina propria (LP) two replicates were performed using four groups of four mice with each group receiving either DSS only, ES only, DSS plus ES or neither DSS or ES (naïve group) (Fig. 5). The DSS only group received a mock injection of PBS instead of ES.

View Article: PubMed Central - PubMed

ABSTRACT

Different reports have highlighted the potential use of helminths and their secretions in the treatment of inflammatory bowel disease (IBD) conditions; however, no reports have investigated their effects at a proteome level. Herein, we characterise the protein expression changes that occur in lamina propria (LP) and the intestinal epithelial cells (IEC) of mice with dextran sulfate sodium (DSS)-induced colitis treated with Ancylostoma caninum excretory/secretory (ES) products using a quantitative proteomic approach. We have shown how parasite products can significantly alter the expression of proteins involved in immune responses, cell death and with an antioxidant activity. Interestingly, significant changes in the expression levels of different mucins were observed in this study. MUC13, a mucin implicated in gastrointestinal homeostasis, was upregulated in the LP of mice with DSS-induced colitis treated with ES, while MUC2, a major component of mucus, was upregulated in the IEC. In addition, A. caninum proteins have an important effect on proteins with antioxidant functions and proteins involved in intestinal homeostasis and tissue integrity and regeneration. Understanding how parasites can ameliorate IBD pathogenesis can help us design novel treatments for autoimmune diseases.

No MeSH data available.


Related in: MedlinePlus