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Pitfalls of invasive blood pressure monitoring using the caudal ventral artery in rats

View Article: PubMed Central - PubMed

ABSTRACT

During rodent experiments, the caudal ventral artery (CVA) is useful for blood pressure (BP) measurement. However, CVA measurements may not reflect the true BP. This study was performed to verify the site-specific accuracy of invasive arterial BP monitoring during surgery in rats. Invasive arterial BP was simultaneously measured in rats via the CVA and the common carotid artery (CCA). The BP values were analysed while the rats were subjected to cooling of the head or tail. Additionally, the rats underwent digital subtraction angiography and histological examination of these arteries. The pressure difference was more significant in the tail cooling group than in the head cooling group. Digital subtraction angiography revealed that angiospasms occurred more frequently in the CVA than in the CCA upon cooling. This phenomenon was supported by histological analysis, which showed that the tunica media area was significantly larger in the CVA than in the CCA. CVA pressure is susceptible to environmental changes and may not accurately reflect the true BP without a strictly controlled laboratory environment. Therefore, understanding the pitfalls of this method is necessary to avoid cooling of the tail during BP measurement.

No MeSH data available.


Thickened tunica media in the caudal ventral artery compared with the common carotid artery.(a,b) Histological sections of the common carotid artery (CCA) from a rat stained with haematoxylin and eosin (a) and Elastica van Gieson stain (b). (c,d) Histological sections of the caudal ventral artery (CVA) from a rat stained with haematoxylin and eosin (c) and Elastica van Gieson stain (d). The tunica media was considerably thicker in the CVA than in the CCA. Scale bar = 200 μm. (e) Comparison of the tunica media area ratio between the CCA and CVA. The tunica media area ratio in the CCA was significantly larger than in the CVA (29.84 ± 2.18% vs. 73.55 ± 5.27%, respectively, P = 0.0001). CCA: common carotid artery, CVA: caudal ventral artery.
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f5: Thickened tunica media in the caudal ventral artery compared with the common carotid artery.(a,b) Histological sections of the common carotid artery (CCA) from a rat stained with haematoxylin and eosin (a) and Elastica van Gieson stain (b). (c,d) Histological sections of the caudal ventral artery (CVA) from a rat stained with haematoxylin and eosin (c) and Elastica van Gieson stain (d). The tunica media was considerably thicker in the CVA than in the CCA. Scale bar = 200 μm. (e) Comparison of the tunica media area ratio between the CCA and CVA. The tunica media area ratio in the CCA was significantly larger than in the CVA (29.84 ± 2.18% vs. 73.55 ± 5.27%, respectively, P = 0.0001). CCA: common carotid artery, CVA: caudal ventral artery.

Mentions: The CCA and CVA of the rats were clearly detected by angiography. We focused on the HwTw and HwTc groups in this analysis. CCA spasms were absent in the control (HwTw) and the head cooling (HcTw) groups (Fig. 4a,b). In contrast, marked constriction of the CVA was observed in the tail cooling (HwTc) group (Fig. 4d) compared to the HwTw group (Fig. 4c). Histological evaluation of the CCA and CVA showed that the tunica media was thicker in the CVA than in the CCA (Fig. 5a–d). The ratio of the percent area of the tunica media to the cross-sectional area of the blood vessel was significantly larger in the CVA than in the CCA (73.55 ± 5.27% vs. 29.84 ± 2.18%, respectively; P = 0.0001; Fig. 5e).


Pitfalls of invasive blood pressure monitoring using the caudal ventral artery in rats
Thickened tunica media in the caudal ventral artery compared with the common carotid artery.(a,b) Histological sections of the common carotid artery (CCA) from a rat stained with haematoxylin and eosin (a) and Elastica van Gieson stain (b). (c,d) Histological sections of the caudal ventral artery (CVA) from a rat stained with haematoxylin and eosin (c) and Elastica van Gieson stain (d). The tunica media was considerably thicker in the CVA than in the CCA. Scale bar = 200 μm. (e) Comparison of the tunica media area ratio between the CCA and CVA. The tunica media area ratio in the CCA was significantly larger than in the CVA (29.84 ± 2.18% vs. 73.55 ± 5.27%, respectively, P = 0.0001). CCA: common carotid artery, CVA: caudal ventral artery.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC5304151&req=5

f5: Thickened tunica media in the caudal ventral artery compared with the common carotid artery.(a,b) Histological sections of the common carotid artery (CCA) from a rat stained with haematoxylin and eosin (a) and Elastica van Gieson stain (b). (c,d) Histological sections of the caudal ventral artery (CVA) from a rat stained with haematoxylin and eosin (c) and Elastica van Gieson stain (d). The tunica media was considerably thicker in the CVA than in the CCA. Scale bar = 200 μm. (e) Comparison of the tunica media area ratio between the CCA and CVA. The tunica media area ratio in the CCA was significantly larger than in the CVA (29.84 ± 2.18% vs. 73.55 ± 5.27%, respectively, P = 0.0001). CCA: common carotid artery, CVA: caudal ventral artery.
Mentions: The CCA and CVA of the rats were clearly detected by angiography. We focused on the HwTw and HwTc groups in this analysis. CCA spasms were absent in the control (HwTw) and the head cooling (HcTw) groups (Fig. 4a,b). In contrast, marked constriction of the CVA was observed in the tail cooling (HwTc) group (Fig. 4d) compared to the HwTw group (Fig. 4c). Histological evaluation of the CCA and CVA showed that the tunica media was thicker in the CVA than in the CCA (Fig. 5a–d). The ratio of the percent area of the tunica media to the cross-sectional area of the blood vessel was significantly larger in the CVA than in the CCA (73.55 ± 5.27% vs. 29.84 ± 2.18%, respectively; P = 0.0001; Fig. 5e).

View Article: PubMed Central - PubMed

ABSTRACT

During rodent experiments, the caudal ventral artery (CVA) is useful for blood pressure (BP) measurement. However, CVA measurements may not reflect the true BP. This study was performed to verify the site-specific accuracy of invasive arterial BP monitoring during surgery in rats. Invasive arterial BP was simultaneously measured in rats via the CVA and the common carotid artery (CCA). The BP values were analysed while the rats were subjected to cooling of the head or tail. Additionally, the rats underwent digital subtraction angiography and histological examination of these arteries. The pressure difference was more significant in the tail cooling group than in the head cooling group. Digital subtraction angiography revealed that angiospasms occurred more frequently in the CVA than in the CCA upon cooling. This phenomenon was supported by histological analysis, which showed that the tunica media area was significantly larger in the CVA than in the CCA. CVA pressure is susceptible to environmental changes and may not accurately reflect the true BP without a strictly controlled laboratory environment. Therefore, understanding the pitfalls of this method is necessary to avoid cooling of the tail during BP measurement.

No MeSH data available.