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Pitfalls of invasive blood pressure monitoring using the caudal ventral artery in rats

View Article: PubMed Central - PubMed

ABSTRACT

During rodent experiments, the caudal ventral artery (CVA) is useful for blood pressure (BP) measurement. However, CVA measurements may not reflect the true BP. This study was performed to verify the site-specific accuracy of invasive arterial BP monitoring during surgery in rats. Invasive arterial BP was simultaneously measured in rats via the CVA and the common carotid artery (CCA). The BP values were analysed while the rats were subjected to cooling of the head or tail. Additionally, the rats underwent digital subtraction angiography and histological examination of these arteries. The pressure difference was more significant in the tail cooling group than in the head cooling group. Digital subtraction angiography revealed that angiospasms occurred more frequently in the CVA than in the CCA upon cooling. This phenomenon was supported by histological analysis, which showed that the tunica media area was significantly larger in the CVA than in the CCA. CVA pressure is susceptible to environmental changes and may not accurately reflect the true BP without a strictly controlled laboratory environment. Therefore, understanding the pitfalls of this method is necessary to avoid cooling of the tail during BP measurement.

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The systolic blood pressure difference was increased by cooling the tail of the rat.The graph shows the change over time under each condition. A significant blood pressure disparity was observed in the tail cooling group (HwTc) compared to the control group (HwTw). *(0.01 < P < 0.05) and **(P < 0.01) based on two-way ANOVA.
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f3: The systolic blood pressure difference was increased by cooling the tail of the rat.The graph shows the change over time under each condition. A significant blood pressure disparity was observed in the tail cooling group (HwTc) compared to the control group (HwTw). *(0.01 < P < 0.05) and **(P < 0.01) based on two-way ANOVA.

Mentions: The time course showed differences in the systolic ABP between the HwTw, HcTw, HwTc, and bleeding groups (Fig. 2). The BP values were clearly different between the four situations. A significant difference in pressure discrepancy was observed between the HwTw and HwTc groups (P = 0.0004). The pressure discrepancy was larger in the bleeding group than in the normovolemic (HwTw) group (P < 0.0001). The pressure discrepancy was evaluated under haemorrhagic shock conditions because this condition could easily cause the peripheral arteries to collapse. The temperature maintained (HwTw) group, the head cooling (HcTw) group, and bleeding group showed less variation regardless of the time course (Supplemental Fig. 1). In contrast, the tail cooling (HwTc) group exhibited considerable variation (Fig. 3). Overall, significant differences were observed between the HwTw and HwTc groups at almost all time points. Tail cooling could also easily cause the tail artery to collapse.


Pitfalls of invasive blood pressure monitoring using the caudal ventral artery in rats
The systolic blood pressure difference was increased by cooling the tail of the rat.The graph shows the change over time under each condition. A significant blood pressure disparity was observed in the tail cooling group (HwTc) compared to the control group (HwTw). *(0.01 < P < 0.05) and **(P < 0.01) based on two-way ANOVA.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC5304151&req=5

f3: The systolic blood pressure difference was increased by cooling the tail of the rat.The graph shows the change over time under each condition. A significant blood pressure disparity was observed in the tail cooling group (HwTc) compared to the control group (HwTw). *(0.01 < P < 0.05) and **(P < 0.01) based on two-way ANOVA.
Mentions: The time course showed differences in the systolic ABP between the HwTw, HcTw, HwTc, and bleeding groups (Fig. 2). The BP values were clearly different between the four situations. A significant difference in pressure discrepancy was observed between the HwTw and HwTc groups (P = 0.0004). The pressure discrepancy was larger in the bleeding group than in the normovolemic (HwTw) group (P < 0.0001). The pressure discrepancy was evaluated under haemorrhagic shock conditions because this condition could easily cause the peripheral arteries to collapse. The temperature maintained (HwTw) group, the head cooling (HcTw) group, and bleeding group showed less variation regardless of the time course (Supplemental Fig. 1). In contrast, the tail cooling (HwTc) group exhibited considerable variation (Fig. 3). Overall, significant differences were observed between the HwTw and HwTc groups at almost all time points. Tail cooling could also easily cause the tail artery to collapse.

View Article: PubMed Central - PubMed

ABSTRACT

During rodent experiments, the caudal ventral artery (CVA) is useful for blood pressure (BP) measurement. However, CVA measurements may not reflect the true BP. This study was performed to verify the site-specific accuracy of invasive arterial BP monitoring during surgery in rats. Invasive arterial BP was simultaneously measured in rats via the CVA and the common carotid artery (CCA). The BP values were analysed while the rats were subjected to cooling of the head or tail. Additionally, the rats underwent digital subtraction angiography and histological examination of these arteries. The pressure difference was more significant in the tail cooling group than in the head cooling group. Digital subtraction angiography revealed that angiospasms occurred more frequently in the CVA than in the CCA upon cooling. This phenomenon was supported by histological analysis, which showed that the tunica media area was significantly larger in the CVA than in the CCA. CVA pressure is susceptible to environmental changes and may not accurately reflect the true BP without a strictly controlled laboratory environment. Therefore, understanding the pitfalls of this method is necessary to avoid cooling of the tail during BP measurement.

No MeSH data available.


Related in: MedlinePlus