Limits...
CD56, HBME-1 and cytokeratin 19 expressions in papillary thyroid carcinoma and nodular thyroid lesions

View Article: PubMed Central - PubMed

ABSTRACT

Background:: Carcinomas of the thyroid follicular epithelium are the most common cancers of the endocrine system. In the diagnosis of thyroid nodules and tumors, the gold standard is histological evaluation. In cases which have morphological overlap, immunohistochemistry is needed for differential diagnosis. The purpose of this study is to investigate the expressions of CD56, HBME-1, cytokeratin 19 (CK19) antibodies in papillary thyroid carcinoma (PTC) and thyroid nodular lesions and their contributions to differential diagnosis.

Materials and methods:: In this study, 47 PTCs (26 follicular variant, 21 classic type) and 26 benign thyroid lesions (15 nodular hyperplasia, 10 follicular adenomas, 1 Hurtle cell adenoma) were analyzed retrospectively. HBME-1, CK19, and CD56 antibodies were performed with immunohistochemical methods. The results were evaluated statistically.

Results:: +3 staining with HBME-1 and CK19 was observed in 72.3% and 83% of patients with PTC. In 95.7% of PTC cases, loss of CD56 expressions in various degrees was identified. A statistically significant difference was detected in HBME-1, CK19, and CD56 expressions between PTCs and benign lesions (P < 0.001).

Conclusion:: In our study, positive staining of HBME-1, CK19, and loosing expression of CD56 that supports malignancy was found and concluded that CD56 is a helpful antibody for the differential diagnosis of benign and malignant lesions and may increase the diagnostic accuracy when used with HBME-1 and CK19.

No MeSH data available.


Related in: MedlinePlus

(a) A case of nodular hyperplasia. There are multiple variably size nodules (one of them was shown). In the same case, no staining with HBME-1 (b) and cytokeratin 19 (c) and strong staining with CD56 (d) (a: H and E×100; b: HBME-1 ×100; c: CK19×100; d: CD56×100)
© Copyright Policy - open-access
Related In: Results  -  Collection

License
getmorefigures.php?uid=PMC5121990&req=5

Figure 4: (a) A case of nodular hyperplasia. There are multiple variably size nodules (one of them was shown). In the same case, no staining with HBME-1 (b) and cytokeratin 19 (c) and strong staining with CD56 (d) (a: H and E×100; b: HBME-1 ×100; c: CK19×100; d: CD56×100)

Mentions: In PTC cases, +3 staining percentages for HBME-1, CK19, and CD56 were 72.3%, 83%, and 4.3%, respectively. Assessment of CD56 staining in the 47 PTC cases showed negative CD56 expression in 32 cases (68.1%), and 43 cases had varying degrees of loss in CD56 expression (95.7%) [Figures 1 and 2]. All of the benign cases showed positive staining with CD56. About 80.8% of these cases had +3 staining [Table 1 and Figures 3, 4]. Between benign and malignant groups, there found a significant difference for percentages of HBME-1, CK19, and CD56 staining with Chi-square test (P < 0.001). The percentages of HBME-1, CK19, and CD56 staining for subtypes of lesions are shown in Table 2.


CD56, HBME-1 and cytokeratin 19 expressions in papillary thyroid carcinoma and nodular thyroid lesions
(a) A case of nodular hyperplasia. There are multiple variably size nodules (one of them was shown). In the same case, no staining with HBME-1 (b) and cytokeratin 19 (c) and strong staining with CD56 (d) (a: H and E×100; b: HBME-1 ×100; c: CK19×100; d: CD56×100)
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC5121990&req=5

Figure 4: (a) A case of nodular hyperplasia. There are multiple variably size nodules (one of them was shown). In the same case, no staining with HBME-1 (b) and cytokeratin 19 (c) and strong staining with CD56 (d) (a: H and E×100; b: HBME-1 ×100; c: CK19×100; d: CD56×100)
Mentions: In PTC cases, +3 staining percentages for HBME-1, CK19, and CD56 were 72.3%, 83%, and 4.3%, respectively. Assessment of CD56 staining in the 47 PTC cases showed negative CD56 expression in 32 cases (68.1%), and 43 cases had varying degrees of loss in CD56 expression (95.7%) [Figures 1 and 2]. All of the benign cases showed positive staining with CD56. About 80.8% of these cases had +3 staining [Table 1 and Figures 3, 4]. Between benign and malignant groups, there found a significant difference for percentages of HBME-1, CK19, and CD56 staining with Chi-square test (P < 0.001). The percentages of HBME-1, CK19, and CD56 staining for subtypes of lesions are shown in Table 2.

View Article: PubMed Central - PubMed

ABSTRACT

Background:: Carcinomas of the thyroid follicular epithelium are the most common cancers of the endocrine system. In the diagnosis of thyroid nodules and tumors, the gold standard is histological evaluation. In cases which have morphological overlap, immunohistochemistry is needed for differential diagnosis. The purpose of this study is to investigate the expressions of CD56, HBME-1, cytokeratin 19 (CK19) antibodies in papillary thyroid carcinoma (PTC) and thyroid nodular lesions and their contributions to differential diagnosis.

Materials and methods:: In this study, 47 PTCs (26 follicular variant, 21 classic type) and 26 benign thyroid lesions (15 nodular hyperplasia, 10 follicular adenomas, 1 Hurtle cell adenoma) were analyzed retrospectively. HBME-1, CK19, and CD56 antibodies were performed with immunohistochemical methods. The results were evaluated statistically.

Results:: +3 staining with HBME-1 and CK19 was observed in 72.3% and 83% of patients with PTC. In 95.7% of PTC cases, loss of CD56 expressions in various degrees was identified. A statistically significant difference was detected in HBME-1, CK19, and CD56 expressions between PTCs and benign lesions (P &lt; 0.001).

Conclusion:: In our study, positive staining of HBME-1, CK19, and loosing expression of CD56 that supports malignancy was found and concluded that CD56 is a helpful antibody for the differential diagnosis of benign and malignant lesions and may increase the diagnostic accuracy when used with HBME-1 and CK19.

No MeSH data available.


Related in: MedlinePlus