Limits...
Composition and biological activities of the aqueous extracts of three scleractinian corals from the Mexican Caribbean: Pseudodiploria strigosa , Porites astreoides and Siderastrea siderea

View Article: PubMed Central - PubMed

ABSTRACT

Background: Scleractinian corals (stony corals) are the most abundant reef-forming cnidarians found in coral reefs throughout the world. Despite their abundance and ecological importance, information about the diversity of their toxins and their biological activities is very scarce. In this study, the chemical composition and the biological activities of the aqueous extracts of Pseudodiploria strigosa, Porites astreoides and Siderastrea siderea, three scleractinian corals from the Mexican Caribbean, have been assessed for the first time.

Methods: Toxicity of the extracts was assessed in crickets; the presence of cytolysins was detected by the hemolysis assay; the vasoconstrictor activity was determined by the isolated rat aortic ring assay; the nociceptive activity was evaluated by the formalin test. The presence of phospholipases A2 (PLA2), serine proteases, and hyaluronidases was determined by enzymatic methods. Low-molecular-weight fractions were obtained by gel filtration chromatography and ultrafiltration.

Results: Extracts from the three species were toxic to crickets, induced hemolysis in human and rat erythrocytes, produced vasoconstriction on isolated rat aortic rings, and presented phospholipase A2 and serine-protease activity. Despite the fact that these corals are not considered to be harmless to humans, the extracts generated significant nociceptive responses. The matrix-assisted laser desorption/ionization time-of-flight (MALDI-TOF) mass spectrometry analysis of the low-molecular-weight fractions revealed the presence of peptides within a mass range of 3000 to 6000 Da. These fractions were toxic to crickets and two of them induced a transitory vasoconstrictor effect on isolated rat aortic rings.

Conclusion: This study suggests that scleractinian corals produce low-molecular-weight peptides that are lethal to crickets and induce vasoconstriction.

No MeSH data available.


Formalin test for assessment of the nociceptive activity of the extracts in rats at a dose of 40 μg protein/paw. Nociceptive behavior in phase 1 (0–10 min post-injection) and phase 2 (10–50 min post-injection) was scored as the amount of time spent licking, flinching, and lifting. *Significant difference (p < 0.05) when compared to the negative group injected with saline solution
© Copyright Policy - OpenAccess
Related In: Results  -  Collection

License 1 - License 2
getmorefigures.php?uid=PMC5121987&req=5

Fig4: Formalin test for assessment of the nociceptive activity of the extracts in rats at a dose of 40 μg protein/paw. Nociceptive behavior in phase 1 (0–10 min post-injection) and phase 2 (10–50 min post-injection) was scored as the amount of time spent licking, flinching, and lifting. *Significant difference (p < 0.05) when compared to the negative group injected with saline solution

Mentions: Evaluation of nociceptive activity by the formalin test showed that unilateral intraplantar injection of the extracts (40 μg of protein) generated significant nociceptive behavior only during the first phase (Fig. 4). As expected, formalin generated significant biphasic nociceptive behavior.Fig. 4


Composition and biological activities of the aqueous extracts of three scleractinian corals from the Mexican Caribbean: Pseudodiploria strigosa , Porites astreoides and Siderastrea siderea
Formalin test for assessment of the nociceptive activity of the extracts in rats at a dose of 40 μg protein/paw. Nociceptive behavior in phase 1 (0–10 min post-injection) and phase 2 (10–50 min post-injection) was scored as the amount of time spent licking, flinching, and lifting. *Significant difference (p < 0.05) when compared to the negative group injected with saline solution
© Copyright Policy - OpenAccess
Related In: Results  -  Collection

License 1 - License 2
Show All Figures
getmorefigures.php?uid=PMC5121987&req=5

Fig4: Formalin test for assessment of the nociceptive activity of the extracts in rats at a dose of 40 μg protein/paw. Nociceptive behavior in phase 1 (0–10 min post-injection) and phase 2 (10–50 min post-injection) was scored as the amount of time spent licking, flinching, and lifting. *Significant difference (p < 0.05) when compared to the negative group injected with saline solution
Mentions: Evaluation of nociceptive activity by the formalin test showed that unilateral intraplantar injection of the extracts (40 μg of protein) generated significant nociceptive behavior only during the first phase (Fig. 4). As expected, formalin generated significant biphasic nociceptive behavior.Fig. 4

View Article: PubMed Central - PubMed

ABSTRACT

Background: Scleractinian corals (stony corals) are the most abundant reef-forming cnidarians found in coral reefs throughout the world. Despite their abundance and ecological importance, information about the diversity of their toxins and their biological activities is very scarce. In this study, the chemical composition and the biological activities of the aqueous extracts of Pseudodiploria strigosa, Porites astreoides and Siderastrea siderea, three scleractinian corals from the Mexican Caribbean, have been assessed for the first time.

Methods: Toxicity of the extracts was assessed in crickets; the presence of cytolysins was detected by the hemolysis assay; the vasoconstrictor activity was determined by the isolated rat aortic ring assay; the nociceptive activity was evaluated by the formalin test. The presence of phospholipases A2 (PLA2), serine proteases, and hyaluronidases was determined by enzymatic methods. Low-molecular-weight fractions were obtained by gel filtration chromatography and ultrafiltration.

Results: Extracts from the three species were toxic to crickets, induced hemolysis in human and rat erythrocytes, produced vasoconstriction on isolated rat aortic rings, and presented phospholipase A2 and serine-protease activity. Despite the fact that these corals are not considered to be harmless to humans, the extracts generated significant nociceptive responses. The matrix-assisted laser desorption/ionization time-of-flight (MALDI-TOF) mass spectrometry analysis of the low-molecular-weight fractions revealed the presence of peptides within a mass range of 3000 to 6000&nbsp;Da. These fractions were toxic to crickets and two of them induced a transitory vasoconstrictor effect on isolated rat aortic rings.

Conclusion: This study suggests that scleractinian corals produce low-molecular-weight peptides that are lethal to crickets and induce vasoconstriction.

No MeSH data available.