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No midterm advantages in the middle term using small intestinal submucosa and human amniotic membrane in Achilles tendon transverse tenotomy

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ABSTRACT

Background: The study was aimed to compare the effects of small intestinal submucosa (SIS) and human amniotic membrane (HAM) on Achilles tendon healing.

Methods: A total of 48 New Zealand white rabbits were divided into two groups. A full-thickness transverse tenotomy was made at the right leg of the rabbits. Then, the laceration site was wrapped with HAM (P/A group) or SIS (P/S group). The ultimate stress (US) and Young’s modulus (E) of the tendons were detected for biomechanical analysis. Histological evaluation was performed using hematoxylin and eosin, immunohistochemical, and immunofluorescent stain. Expression of collagen I was detected by western blot analysis, and levels of inflammatory cytokines IL-1β, IL-6, and TNF-α were measured. Finally, adhesion formation was evaluated.

Results: There were no significant differences in filamentous adhesion, cross-sectional areas of the laceration sites, levels of inflammatory response, and collagen type I expression between the P/A and P/S groups (p > 0.05). Compared with the P/A group, the US and E values were significantly higher in the P/S group at day 7 (p < 0.05) and at day 14 (p < 0.05). In addition, vascularity was significantly higher in the P/S group than that in the P/A group at day 3 (p < 0.05), day 7 (p < 0.01), and day 9 (p < 0.05).

Conclusions: SIS showed superior biomechanical properties and neovascularization over HAM in treatment of Achilles tendon injury in the early stage of healing.

No MeSH data available.


The ultimate stress and Young’s modulus of the tendons in the P/A and P/S groups at days 7, 14, 28, and 56. *p < 0.05
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Fig3: The ultimate stress and Young’s modulus of the tendons in the P/A and P/S groups at days 7, 14, 28, and 56. *p < 0.05

Mentions: The biomechanical property of the tendons presented by US and E were shown in Fig. 3. Compared with the P/A group, the US and E values were significantly higher in the P/S group at day 7 (p < 0.05) and at day 14 (p < 0.05). There was no significant difference of US or E between the two groups at day 28 and day 56 (p > 0.05).Fig. 3


No midterm advantages in the middle term using small intestinal submucosa and human amniotic membrane in Achilles tendon transverse tenotomy
The ultimate stress and Young’s modulus of the tendons in the P/A and P/S groups at days 7, 14, 28, and 56. *p < 0.05
© Copyright Policy - OpenAccess
Related In: Results  -  Collection

License 1 - License 2
Show All Figures
getmorefigures.php?uid=PMC5121975&req=5

Fig3: The ultimate stress and Young’s modulus of the tendons in the P/A and P/S groups at days 7, 14, 28, and 56. *p < 0.05
Mentions: The biomechanical property of the tendons presented by US and E were shown in Fig. 3. Compared with the P/A group, the US and E values were significantly higher in the P/S group at day 7 (p < 0.05) and at day 14 (p < 0.05). There was no significant difference of US or E between the two groups at day 28 and day 56 (p > 0.05).Fig. 3

View Article: PubMed Central - PubMed

ABSTRACT

Background: The study was aimed to compare the effects of small intestinal submucosa (SIS) and human amniotic membrane (HAM) on Achilles tendon healing.

Methods: A total of 48 New Zealand white rabbits were divided into two groups. A full-thickness transverse tenotomy was made at the right leg of the rabbits. Then, the laceration site was wrapped with HAM (P/A group) or SIS (P/S group). The ultimate stress (US) and Young&rsquo;s modulus (E) of the tendons were detected for biomechanical analysis. Histological evaluation was performed using hematoxylin and eosin, immunohistochemical, and immunofluorescent stain. Expression of collagen I was detected by western blot analysis, and levels of inflammatory cytokines IL-1&beta;, IL-6, and TNF-&alpha; were measured. Finally, adhesion formation was evaluated.

Results: There were no significant differences in filamentous adhesion, cross-sectional areas of the laceration sites, levels of inflammatory response, and collagen type I expression between the P/A and P/S groups (p&thinsp;&gt;&thinsp;0.05). Compared with the P/A group, the US and E values were significantly higher in the P/S group at day 7 (p&thinsp;&lt;&thinsp;0.05) and at day 14 (p&thinsp;&lt;&thinsp;0.05). In addition, vascularity was significantly higher in the P/S group than that in the P/A group at day 3 (p&thinsp;&lt;&thinsp;0.05), day 7 (p&thinsp;&lt;&thinsp;0.01), and day 9 (p&thinsp;&lt;&thinsp;0.05).

Conclusions: SIS showed superior biomechanical properties and neovascularization over HAM in treatment of Achilles tendon injury in the early stage of healing.

No MeSH data available.