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High expression of GNA13 is associated with poor prognosis in hepatocellular carcinoma

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ABSTRACT

Guanine nucleotide binding protein alpha 13 (GNA13) has been found to play critical roles in the development of several human cancers. However, little is known about GNA13 expression and its clinical significance in hepatocellular carcinoma (HCC). In our study, GNA13 was reported to be significantly up-regulated in HCC tissues, and this was correlated with several clinicopathological parameters, including tumor multiplicity (P = 0.004), TNM stage (P = 0.002), and BCLC stage (P = 0.010). Further Cox regression analysis suggested that GNA13 expression was an independent prognostic factor for overall survival (P = 0.014) and disease-free survival (P = 0.005). Moreover, we found that overexpression of GNA13 couldn’t promote cell proliferation in vitro, but could significantly increase the invasion ability of HCC cells. Together, our study demonstrates GNA13 may be served as a prognostic biomarker for HCC patients after curative hepatectomy, in which high expression of GNA13 suggests poor prognosis of HCC patients.

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Effect of GNA13 overexpression on cell proliferation and invasion in vitro.(a) Overexpression of GNA13 could not promote the proliferation of HepG2 and SMMC-7721 cells in vitro as determined by MTT assay. (b) In the invasion assay, the cell invasion ability was significantly increased after overexpression of GNA13 in HepG2 and SMMC-7721 cells when compared with Vector control. Bars represent the mean ± SD of three independent experiments. *P < 0.05.
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f5: Effect of GNA13 overexpression on cell proliferation and invasion in vitro.(a) Overexpression of GNA13 could not promote the proliferation of HepG2 and SMMC-7721 cells in vitro as determined by MTT assay. (b) In the invasion assay, the cell invasion ability was significantly increased after overexpression of GNA13 in HepG2 and SMMC-7721 cells when compared with Vector control. Bars represent the mean ± SD of three independent experiments. *P < 0.05.

Mentions: To determine the effect of GNA13 on cell proliferation and invasion in HCC cells, we established stable GNA13-overexpressing HCC cells, HepG2 and SMMC-7721. In the MTT assay, overexpression of GNA13 could not promote cell proliferation in both HepG2 and SMMC-7721 cells in vitro compared to the vector controls group (Fig. 5a). By contrast, the invasion assay proved that the cell invasion ability was significantly increased after overexpression of GNA13 expression in HepG2 and SMMC-7721 cells when compared with vector controls (Fig. 5b).


High expression of GNA13 is associated with poor prognosis in hepatocellular carcinoma
Effect of GNA13 overexpression on cell proliferation and invasion in vitro.(a) Overexpression of GNA13 could not promote the proliferation of HepG2 and SMMC-7721 cells in vitro as determined by MTT assay. (b) In the invasion assay, the cell invasion ability was significantly increased after overexpression of GNA13 in HepG2 and SMMC-7721 cells when compared with Vector control. Bars represent the mean ± SD of three independent experiments. *P < 0.05.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC5121652&req=5

f5: Effect of GNA13 overexpression on cell proliferation and invasion in vitro.(a) Overexpression of GNA13 could not promote the proliferation of HepG2 and SMMC-7721 cells in vitro as determined by MTT assay. (b) In the invasion assay, the cell invasion ability was significantly increased after overexpression of GNA13 in HepG2 and SMMC-7721 cells when compared with Vector control. Bars represent the mean ± SD of three independent experiments. *P < 0.05.
Mentions: To determine the effect of GNA13 on cell proliferation and invasion in HCC cells, we established stable GNA13-overexpressing HCC cells, HepG2 and SMMC-7721. In the MTT assay, overexpression of GNA13 could not promote cell proliferation in both HepG2 and SMMC-7721 cells in vitro compared to the vector controls group (Fig. 5a). By contrast, the invasion assay proved that the cell invasion ability was significantly increased after overexpression of GNA13 expression in HepG2 and SMMC-7721 cells when compared with vector controls (Fig. 5b).

View Article: PubMed Central - PubMed

ABSTRACT

Guanine nucleotide binding protein alpha 13 (GNA13) has been found to play critical roles in the development of several human cancers. However, little is known about GNA13 expression and its clinical significance in hepatocellular carcinoma (HCC). In our study, GNA13 was reported to be significantly up-regulated in HCC tissues, and this was correlated with several clinicopathological parameters, including tumor multiplicity (P&thinsp;=&thinsp;0.004), TNM stage (P&thinsp;=&thinsp;0.002), and BCLC stage (P&thinsp;=&thinsp;0.010). Further Cox regression analysis suggested that GNA13 expression was an independent prognostic factor for overall survival (P&thinsp;=&thinsp;0.014) and disease-free survival (P&thinsp;=&thinsp;0.005). Moreover, we found that overexpression of GNA13 couldn&rsquo;t promote cell proliferation in vitro, but could significantly increase the invasion ability of HCC cells. Together, our study demonstrates GNA13 may be served as a prognostic biomarker for HCC patients after curative hepatectomy, in which high expression of GNA13 suggests poor prognosis of HCC patients.

No MeSH data available.


Related in: MedlinePlus