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Spatial mapping of lichen specialized metabolites using LDI-MSI: chemical ecology issues for Ophioparma ventosa

View Article: PubMed Central - PubMed

ABSTRACT

Imaging mass spectrometry techniques have become a powerful strategy to assess the spatial distribution of metabolites in biological systems. Based on auto-ionisability of lichen metabolites using LDI-MS, we herein image the distribution of major secondary metabolites (specialized metabolites) from the lichen Ophioparma ventosa by LDI-MSI (Mass Spectrometry Imaging). Such technologies offer tremendous opportunities to discuss the role of natural products through spatial mapping, their distribution patterns being consistent with previous chemical ecology reports. A special attention was dedicated to miriquidic acid, an unexpected molecule we first reported in Ophioparma ventosa. The analytical strategy presented herein offers new perspectives to access the sharp distribution of lichen metabolites from regular razor blade-sectioned slices.

No MeSH data available.


Related in: MedlinePlus

From left to right: Molecular images of usnic acid, divaricatic acid, thamnolic acid, miriquidic acid and all overlaid ions in a hand-cut piece of an apotheciate piece of thallus from the Styria sample of Ophioparma ventosa.Intensities of ions in the imaged spots are color coded using a heat map with relative intensities given as indicated on the color scale bars.
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f5: From left to right: Molecular images of usnic acid, divaricatic acid, thamnolic acid, miriquidic acid and all overlaid ions in a hand-cut piece of an apotheciate piece of thallus from the Styria sample of Ophioparma ventosa.Intensities of ions in the imaged spots are color coded using a heat map with relative intensities given as indicated on the color scale bars.

Mentions: In situ assessment of O. ventosa specialized metabolites is displayed on two series of mass spectrometric images from the two sampling sites (Tyrol: Fig. 4 and S4 and Styria: Fig. 5 and S5). A first outcome is that LDI-MSI afforded ion images of similar qualities from both 40 μm thick cryosectioned slices and ca. 100 μm hand-cut sections. Then, the distribution patterns retrieved from the lichens of both sampling sites and from either apotheciate or non-apotheciate pieces of thallus are similar. Specialized metabolites could be imaged with a resolution of 50 μm, showing organized and specific spatial allocations for each compound, which do not overlay.


Spatial mapping of lichen specialized metabolites using LDI-MSI: chemical ecology issues for Ophioparma ventosa
From left to right: Molecular images of usnic acid, divaricatic acid, thamnolic acid, miriquidic acid and all overlaid ions in a hand-cut piece of an apotheciate piece of thallus from the Styria sample of Ophioparma ventosa.Intensities of ions in the imaged spots are color coded using a heat map with relative intensities given as indicated on the color scale bars.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC5121634&req=5

f5: From left to right: Molecular images of usnic acid, divaricatic acid, thamnolic acid, miriquidic acid and all overlaid ions in a hand-cut piece of an apotheciate piece of thallus from the Styria sample of Ophioparma ventosa.Intensities of ions in the imaged spots are color coded using a heat map with relative intensities given as indicated on the color scale bars.
Mentions: In situ assessment of O. ventosa specialized metabolites is displayed on two series of mass spectrometric images from the two sampling sites (Tyrol: Fig. 4 and S4 and Styria: Fig. 5 and S5). A first outcome is that LDI-MSI afforded ion images of similar qualities from both 40 μm thick cryosectioned slices and ca. 100 μm hand-cut sections. Then, the distribution patterns retrieved from the lichens of both sampling sites and from either apotheciate or non-apotheciate pieces of thallus are similar. Specialized metabolites could be imaged with a resolution of 50 μm, showing organized and specific spatial allocations for each compound, which do not overlay.

View Article: PubMed Central - PubMed

ABSTRACT

Imaging mass spectrometry techniques have become a powerful strategy to assess the spatial distribution of metabolites in biological systems. Based on auto-ionisability of lichen metabolites using LDI-MS, we herein image the distribution of major secondary metabolites (specialized metabolites) from the lichen Ophioparma ventosa by LDI-MSI (Mass Spectrometry Imaging). Such technologies offer tremendous opportunities to discuss the role of natural products through spatial mapping, their distribution patterns being consistent with previous chemical ecology reports. A special attention was dedicated to miriquidic acid, an unexpected molecule we first reported in Ophioparma ventosa. The analytical strategy presented herein offers new perspectives to access the sharp distribution of lichen metabolites from regular razor blade-sectioned slices.

No MeSH data available.


Related in: MedlinePlus