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Adipose Stem Cells Display Higher Regenerative Capacities and More Adaptable Electro-Kinetic Properties Compared to Bone Marrow-Derived Mesenchymal Stromal Cells

View Article: PubMed Central - PubMed

ABSTRACT

Adipose stem cells (ASCs) have recently emerged as a more viable source for clinical applications, compared to bone-marrow mesenchymal stromal cells (BM-MSCs) because of their abundance and easy access. In this study we evaluated the regenerative potency of ASCs compared to BM-MSCs. Furthermore, we compared the dielectric and electro-kinetic properties of both types of cells using a novel Dielectrophoresis (DEP) microfluidic platform based on a printed circuit board (PCB) technology. Our data show that ASCs were more effective than BM-MSCs in promoting neovascularization in an animal model of hind-limb ischemia. When compared to BM-MSCs, ASCs displayed higher resistance to hypoxia-induced apoptosis, and to oxidative stress-induced senescence, and showed more potent proangiogenic activity. mRNA expression analysis showed that ASCs had a higher expression of Oct4 and VEGF than BM-MSCs. Furthermore, ASCs showed a remarkably higher telomerase activity. Analysis of the electro-kinetic properties showed that ASCs displayed different traveling wave velocity and rotational speed compared to BM-MSCs. Interestingly, ASCs seem to develop an adaptive response when exposed to repeated electric field stimulation. These data provide new insights into the physiology of ASCs, and evidence to their potential superior potency compared to marrow MSCs as a source of stem cells.

No MeSH data available.


Traveling wave velocity of (A) ASCs and (B) BM-MSCs at 8 MHz during different time points: Snap shots of (C) ASCs and (D) BM-MSCs at frequency 8 MHz, 10 Vpp square wave.
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f6: Traveling wave velocity of (A) ASCs and (B) BM-MSCs at 8 MHz during different time points: Snap shots of (C) ASCs and (D) BM-MSCs at frequency 8 MHz, 10 Vpp square wave.

Mentions: The traveling wave speed was measured for both ASCs and BM-MSCs at frequency of 8 MHz. The speed for each type of cells versus time was measured and plotted (Fig. 6A and B). ASCs displayed a higher starting velocity than BM-MSCs, while the rate of decrease for ASCs was faster than that for BM-MSCs (Fig. 6A and B). Supplementary Videos 1 & 2 show the travelling velocity of ASCs and BM-MSCs, respectively.


Adipose Stem Cells Display Higher Regenerative Capacities and More Adaptable Electro-Kinetic Properties Compared to Bone Marrow-Derived Mesenchymal Stromal Cells
Traveling wave velocity of (A) ASCs and (B) BM-MSCs at 8 MHz during different time points: Snap shots of (C) ASCs and (D) BM-MSCs at frequency 8 MHz, 10 Vpp square wave.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC5121630&req=5

f6: Traveling wave velocity of (A) ASCs and (B) BM-MSCs at 8 MHz during different time points: Snap shots of (C) ASCs and (D) BM-MSCs at frequency 8 MHz, 10 Vpp square wave.
Mentions: The traveling wave speed was measured for both ASCs and BM-MSCs at frequency of 8 MHz. The speed for each type of cells versus time was measured and plotted (Fig. 6A and B). ASCs displayed a higher starting velocity than BM-MSCs, while the rate of decrease for ASCs was faster than that for BM-MSCs (Fig. 6A and B). Supplementary Videos 1 & 2 show the travelling velocity of ASCs and BM-MSCs, respectively.

View Article: PubMed Central - PubMed

ABSTRACT

Adipose stem cells (ASCs) have recently emerged as a more viable source for clinical applications, compared to bone-marrow mesenchymal stromal cells (BM-MSCs) because of their abundance and easy access. In this study we evaluated the regenerative potency of ASCs compared to BM-MSCs. Furthermore, we compared the dielectric and electro-kinetic properties of both types of cells using a novel Dielectrophoresis (DEP) microfluidic platform based on a printed circuit board (PCB) technology. Our data show that ASCs were more effective than BM-MSCs in promoting neovascularization in an animal model of hind-limb ischemia. When compared to BM-MSCs, ASCs displayed higher resistance to hypoxia-induced apoptosis, and to oxidative stress-induced senescence, and showed more potent proangiogenic activity. mRNA expression analysis showed that ASCs had a higher expression of Oct4 and VEGF than BM-MSCs. Furthermore, ASCs showed a remarkably higher telomerase activity. Analysis of the electro-kinetic properties showed that ASCs displayed different traveling wave velocity and rotational speed compared to BM-MSCs. Interestingly, ASCs seem to develop an adaptive response when exposed to repeated electric field stimulation. These data provide new insights into the physiology of ASCs, and evidence to their potential superior potency compared to marrow MSCs as a source of stem cells.

No MeSH data available.