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Adipose Stem Cells Display Higher Regenerative Capacities and More Adaptable Electro-Kinetic Properties Compared to Bone Marrow-Derived Mesenchymal Stromal Cells

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ABSTRACT

Adipose stem cells (ASCs) have recently emerged as a more viable source for clinical applications, compared to bone-marrow mesenchymal stromal cells (BM-MSCs) because of their abundance and easy access. In this study we evaluated the regenerative potency of ASCs compared to BM-MSCs. Furthermore, we compared the dielectric and electro-kinetic properties of both types of cells using a novel Dielectrophoresis (DEP) microfluidic platform based on a printed circuit board (PCB) technology. Our data show that ASCs were more effective than BM-MSCs in promoting neovascularization in an animal model of hind-limb ischemia. When compared to BM-MSCs, ASCs displayed higher resistance to hypoxia-induced apoptosis, and to oxidative stress-induced senescence, and showed more potent proangiogenic activity. mRNA expression analysis showed that ASCs had a higher expression of Oct4 and VEGF than BM-MSCs. Furthermore, ASCs showed a remarkably higher telomerase activity. Analysis of the electro-kinetic properties showed that ASCs displayed different traveling wave velocity and rotational speed compared to BM-MSCs. Interestingly, ASCs seem to develop an adaptive response when exposed to repeated electric field stimulation. These data provide new insights into the physiology of ASCs, and evidence to their potential superior potency compared to marrow MSCs as a source of stem cells.

No MeSH data available.


ASCs have a more potent pro-angiogenic effects when compared to BM-MSCs).Upper panel is the phase contrast image of the field shown on the below panel (A). Tubular structures were photographed (A and B) and then quantified by counting the number of branch points (C). In vitro tube formation assay (A and B) and tube branch points (C). Representative images are displayed and data are shown as mean ± S.D. (error bars) of counted tube branch points from 4 microscopic fields of three independent replicates. ***p < 0.005. Scale bars, 500 μm.
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f4: ASCs have a more potent pro-angiogenic effects when compared to BM-MSCs).Upper panel is the phase contrast image of the field shown on the below panel (A). Tubular structures were photographed (A and B) and then quantified by counting the number of branch points (C). In vitro tube formation assay (A and B) and tube branch points (C). Representative images are displayed and data are shown as mean ± S.D. (error bars) of counted tube branch points from 4 microscopic fields of three independent replicates. ***p < 0.005. Scale bars, 500 μm.

Mentions: The angiogenic capacities of both BM-MSCs and ASCs were assessed using an in vitro angiogenesis tube formation after culture on Geltrex-coated plates in large vessel endothelial-supplemented Medium 200. As shown in Fig. 4A, within 12 hours, ASCs formed more extensive networks of hallow, capillary tube-like structures, compared to BM-MSCs. The number of branching points was significantly higher in ASCs than BM-MSCs (p < 0.005, Fig. 4B).


Adipose Stem Cells Display Higher Regenerative Capacities and More Adaptable Electro-Kinetic Properties Compared to Bone Marrow-Derived Mesenchymal Stromal Cells
ASCs have a more potent pro-angiogenic effects when compared to BM-MSCs).Upper panel is the phase contrast image of the field shown on the below panel (A). Tubular structures were photographed (A and B) and then quantified by counting the number of branch points (C). In vitro tube formation assay (A and B) and tube branch points (C). Representative images are displayed and data are shown as mean ± S.D. (error bars) of counted tube branch points from 4 microscopic fields of three independent replicates. ***p < 0.005. Scale bars, 500 μm.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC5121630&req=5

f4: ASCs have a more potent pro-angiogenic effects when compared to BM-MSCs).Upper panel is the phase contrast image of the field shown on the below panel (A). Tubular structures were photographed (A and B) and then quantified by counting the number of branch points (C). In vitro tube formation assay (A and B) and tube branch points (C). Representative images are displayed and data are shown as mean ± S.D. (error bars) of counted tube branch points from 4 microscopic fields of three independent replicates. ***p < 0.005. Scale bars, 500 μm.
Mentions: The angiogenic capacities of both BM-MSCs and ASCs were assessed using an in vitro angiogenesis tube formation after culture on Geltrex-coated plates in large vessel endothelial-supplemented Medium 200. As shown in Fig. 4A, within 12 hours, ASCs formed more extensive networks of hallow, capillary tube-like structures, compared to BM-MSCs. The number of branching points was significantly higher in ASCs than BM-MSCs (p < 0.005, Fig. 4B).

View Article: PubMed Central - PubMed

ABSTRACT

Adipose stem cells (ASCs) have recently emerged as a more viable source for clinical applications, compared to bone-marrow mesenchymal stromal cells (BM-MSCs) because of their abundance and easy access. In this study we evaluated the regenerative potency of ASCs compared to BM-MSCs. Furthermore, we compared the dielectric and electro-kinetic properties of both types of cells using a novel Dielectrophoresis (DEP) microfluidic platform based on a printed circuit board (PCB) technology. Our data show that ASCs were more effective than BM-MSCs in promoting neovascularization in an animal model of hind-limb ischemia. When compared to BM-MSCs, ASCs displayed higher resistance to hypoxia-induced apoptosis, and to oxidative stress-induced senescence, and showed more potent proangiogenic activity. mRNA expression analysis showed that ASCs had a higher expression of Oct4 and VEGF than BM-MSCs. Furthermore, ASCs showed a remarkably higher telomerase activity. Analysis of the electro-kinetic properties showed that ASCs displayed different traveling wave velocity and rotational speed compared to BM-MSCs. Interestingly, ASCs seem to develop an adaptive response when exposed to repeated electric field stimulation. These data provide new insights into the physiology of ASCs, and evidence to their potential superior potency compared to marrow MSCs as a source of stem cells.

No MeSH data available.