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T-Cadherin Expression in the Epidermis and Adnexal Structures of Normal Skin

View Article: PubMed Central - PubMed

ABSTRACT

Background: T-cadherin is an atypical glycosylphosphatidylinositol-anchored member of the cadherin superfamily of adhesion molecules. The role of T-cadherin in biology of the skin is poorly understood. Expression of T-cadherin in basal keratinocytes and dermal blood vessels of the healthy epidermis has been demonstrated, but studies on expression in skin appendages are rare.

Methods: We conducted an immunohistochemical analysis of T-cadherin expression in the epidermis and adnexal structures of normal skin.

Results: T-cadherin expression is restricted to basal keratinocytes of the epidermis. The basal cell layer of sebaceous glands was T-cadherin positive, whereas sebocytes were negative. Within apocrine glands, only myoepithelial cells were T-cadherin positive. In contrast, both the secretory coils and excretory ducts of eccrine glands were T-cadherin positive. In terminal hair follicles, the outer root sheath layers strongly expressed T-cadherin throughout different regions of the follicle, with the strongest immunoreactivity at the bulge and suprabulbar regions. T-cadherin and CK15 stem cell marker similarly localized within the bulge and suprabulbar region. T-cadherin and CD34 stem cell marker similarly localized at the suprabulbar level.

Conclusion: The specific patterns of T-cadherin expression in the epidermis and adnexal structures suggest an important guardian role in skin homeostasis.

No MeSH data available.


Expression of T-cadherin, CK15, and CD34 in the infundibulum and isthmus in serial sections of terminal hair follicles. a The ORS layer of the hair follicle infundibulum demonstrates positive membranous staining for T-cadherin and CK15, whereas no staining for CD34 is present. b Note that T-cadherin immunoreactivity prominently marks ORS cells within the lower isthmus near the arrector pili muscle (indicated by asterisks) attachment site of the hair follicle known as the bulge. T-cadherin and CK15 stem cell marker similarly localized within the bulge and suprabulbar region. T-cadherin and CD34 stem cell marker similarly localized at the suprabulbar level.
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Figure 5: Expression of T-cadherin, CK15, and CD34 in the infundibulum and isthmus in serial sections of terminal hair follicles. a The ORS layer of the hair follicle infundibulum demonstrates positive membranous staining for T-cadherin and CK15, whereas no staining for CD34 is present. b Note that T-cadherin immunoreactivity prominently marks ORS cells within the lower isthmus near the arrector pili muscle (indicated by asterisks) attachment site of the hair follicle known as the bulge. T-cadherin and CK15 stem cell marker similarly localized within the bulge and suprabulbar region. T-cadherin and CD34 stem cell marker similarly localized at the suprabulbar level.

Mentions: In order to define the distribution for T-cadherin in terminal hair follicles, we used vertical consecutive sections of the hair follicles and compared the localization of T-cadherin with that of two usually used stem cell markers, CD34 and CK15. In terminal hair follicles, the basal cell layer of the epidermis and follicular infundibulum was strongly positive for T-cadherin and CK15 but negative for CD34. CD34 was strongly expressed in the endothelial cell and perifollicular spindle-shaped cells (Fig. 5a). A strong staining for T-cadherin was observed in the outer root sheath (ORS) layers within the isthmus of the follicle: the distribution was typically global with staining intensity being more prominent on the apical surface and at intercellular contacts of cells (Fig. 5b). The area of the ORS around the site where the arrector pili muscle attaches to the hair follicle (otherwise known as the bulge) demonstrated intense staining for T-cadherin (Fig. 5b). In contrast, the inner root sheath of the hair follicles was T-cadherin negative. Strong CK15 immunoreactivity was found in the ORS of the isthmus and with increasing staining intensity at the site of attachment of the arrector pili muscle at the bulge level (Fig. 5b). CD34 staining was not observed in the ORS within the level of the isthmus (Fig. 5b).


T-Cadherin Expression in the Epidermis and Adnexal Structures of Normal Skin
Expression of T-cadherin, CK15, and CD34 in the infundibulum and isthmus in serial sections of terminal hair follicles. a The ORS layer of the hair follicle infundibulum demonstrates positive membranous staining for T-cadherin and CK15, whereas no staining for CD34 is present. b Note that T-cadherin immunoreactivity prominently marks ORS cells within the lower isthmus near the arrector pili muscle (indicated by asterisks) attachment site of the hair follicle known as the bulge. T-cadherin and CK15 stem cell marker similarly localized within the bulge and suprabulbar region. T-cadherin and CD34 stem cell marker similarly localized at the suprabulbar level.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
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getmorefigures.php?uid=PMC5121566&req=5

Figure 5: Expression of T-cadherin, CK15, and CD34 in the infundibulum and isthmus in serial sections of terminal hair follicles. a The ORS layer of the hair follicle infundibulum demonstrates positive membranous staining for T-cadherin and CK15, whereas no staining for CD34 is present. b Note that T-cadherin immunoreactivity prominently marks ORS cells within the lower isthmus near the arrector pili muscle (indicated by asterisks) attachment site of the hair follicle known as the bulge. T-cadherin and CK15 stem cell marker similarly localized within the bulge and suprabulbar region. T-cadherin and CD34 stem cell marker similarly localized at the suprabulbar level.
Mentions: In order to define the distribution for T-cadherin in terminal hair follicles, we used vertical consecutive sections of the hair follicles and compared the localization of T-cadherin with that of two usually used stem cell markers, CD34 and CK15. In terminal hair follicles, the basal cell layer of the epidermis and follicular infundibulum was strongly positive for T-cadherin and CK15 but negative for CD34. CD34 was strongly expressed in the endothelial cell and perifollicular spindle-shaped cells (Fig. 5a). A strong staining for T-cadherin was observed in the outer root sheath (ORS) layers within the isthmus of the follicle: the distribution was typically global with staining intensity being more prominent on the apical surface and at intercellular contacts of cells (Fig. 5b). The area of the ORS around the site where the arrector pili muscle attaches to the hair follicle (otherwise known as the bulge) demonstrated intense staining for T-cadherin (Fig. 5b). In contrast, the inner root sheath of the hair follicles was T-cadherin negative. Strong CK15 immunoreactivity was found in the ORS of the isthmus and with increasing staining intensity at the site of attachment of the arrector pili muscle at the bulge level (Fig. 5b). CD34 staining was not observed in the ORS within the level of the isthmus (Fig. 5b).

View Article: PubMed Central - PubMed

ABSTRACT

Background: T-cadherin is an atypical glycosylphosphatidylinositol-anchored member of the cadherin superfamily of adhesion molecules. The role of T-cadherin in biology of the skin is poorly understood. Expression of T-cadherin in basal keratinocytes and dermal blood vessels of the healthy epidermis has been demonstrated, but studies on expression in skin appendages are rare.

Methods: We conducted an immunohistochemical analysis of T-cadherin expression in the epidermis and adnexal structures of normal skin.

Results: T-cadherin expression is restricted to basal keratinocytes of the epidermis. The basal cell layer of sebaceous glands was T-cadherin positive, whereas sebocytes were negative. Within apocrine glands, only myoepithelial cells were T-cadherin positive. In contrast, both the secretory coils and excretory ducts of eccrine glands were T-cadherin positive. In terminal hair follicles, the outer root sheath layers strongly expressed T-cadherin throughout different regions of the follicle, with the strongest immunoreactivity at the bulge and suprabulbar regions. T-cadherin and CK15 stem cell marker similarly localized within the bulge and suprabulbar region. T-cadherin and CD34 stem cell marker similarly localized at the suprabulbar level.

Conclusion: The specific patterns of T-cadherin expression in the epidermis and adnexal structures suggest an important guardian role in skin homeostasis.

No MeSH data available.