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T-Cadherin Expression in the Epidermis and Adnexal Structures of Normal Skin

View Article: PubMed Central - PubMed

ABSTRACT

Background: T-cadherin is an atypical glycosylphosphatidylinositol-anchored member of the cadherin superfamily of adhesion molecules. The role of T-cadherin in biology of the skin is poorly understood. Expression of T-cadherin in basal keratinocytes and dermal blood vessels of the healthy epidermis has been demonstrated, but studies on expression in skin appendages are rare.

Methods: We conducted an immunohistochemical analysis of T-cadherin expression in the epidermis and adnexal structures of normal skin.

Results: T-cadherin expression is restricted to basal keratinocytes of the epidermis. The basal cell layer of sebaceous glands was T-cadherin positive, whereas sebocytes were negative. Within apocrine glands, only myoepithelial cells were T-cadherin positive. In contrast, both the secretory coils and excretory ducts of eccrine glands were T-cadherin positive. In terminal hair follicles, the outer root sheath layers strongly expressed T-cadherin throughout different regions of the follicle, with the strongest immunoreactivity at the bulge and suprabulbar regions. T-cadherin and CK15 stem cell marker similarly localized within the bulge and suprabulbar region. T-cadherin and CD34 stem cell marker similarly localized at the suprabulbar level.

Conclusion: The specific patterns of T-cadherin expression in the epidermis and adnexal structures suggest an important guardian role in skin homeostasis.

No MeSH data available.


T-cadherin expression in eccrine glands. In the normal eccrine glands, the secretory cells in the secretory coils of the glands (a) typically express T-cadherin over the entire cell body. Note the myoepithelial cells of the secretory portion expressing T-cadherin (arrowheads). The inner layer of epithelial cells lining the excretory ducts of eccrine glands (b) exhibit pronounced luminal staining for T-cadherin (arrowheads).
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Figure 3: T-cadherin expression in eccrine glands. In the normal eccrine glands, the secretory cells in the secretory coils of the glands (a) typically express T-cadherin over the entire cell body. Note the myoepithelial cells of the secretory portion expressing T-cadherin (arrowheads). The inner layer of epithelial cells lining the excretory ducts of eccrine glands (b) exhibit pronounced luminal staining for T-cadherin (arrowheads).

Mentions: In the eccrine glands, moderately to strongly positive cytoplasmic and membranous staining for T-cadherin was found in the majority of the single layer of cells within the secretory portion. T-cadherin was also strongly expressed on the outer myoepithelial cells surrounding the coiled gland (Fig. 3a). The eccrine ducts consisting of a double layer of cells exhibited a strong membranous staining on the inner layer of cuticular cells, although infrequently, a few cells with cytoplasmic staining were seen (Fig. 3b).


T-Cadherin Expression in the Epidermis and Adnexal Structures of Normal Skin
T-cadherin expression in eccrine glands. In the normal eccrine glands, the secretory cells in the secretory coils of the glands (a) typically express T-cadherin over the entire cell body. Note the myoepithelial cells of the secretory portion expressing T-cadherin (arrowheads). The inner layer of epithelial cells lining the excretory ducts of eccrine glands (b) exhibit pronounced luminal staining for T-cadherin (arrowheads).
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC5121566&req=5

Figure 3: T-cadherin expression in eccrine glands. In the normal eccrine glands, the secretory cells in the secretory coils of the glands (a) typically express T-cadherin over the entire cell body. Note the myoepithelial cells of the secretory portion expressing T-cadherin (arrowheads). The inner layer of epithelial cells lining the excretory ducts of eccrine glands (b) exhibit pronounced luminal staining for T-cadherin (arrowheads).
Mentions: In the eccrine glands, moderately to strongly positive cytoplasmic and membranous staining for T-cadherin was found in the majority of the single layer of cells within the secretory portion. T-cadherin was also strongly expressed on the outer myoepithelial cells surrounding the coiled gland (Fig. 3a). The eccrine ducts consisting of a double layer of cells exhibited a strong membranous staining on the inner layer of cuticular cells, although infrequently, a few cells with cytoplasmic staining were seen (Fig. 3b).

View Article: PubMed Central - PubMed

ABSTRACT

Background: T-cadherin is an atypical glycosylphosphatidylinositol-anchored member of the cadherin superfamily of adhesion molecules. The role of T-cadherin in biology of the skin is poorly understood. Expression of T-cadherin in basal keratinocytes and dermal blood vessels of the healthy epidermis has been demonstrated, but studies on expression in skin appendages are rare.

Methods: We conducted an immunohistochemical analysis of T-cadherin expression in the epidermis and adnexal structures of normal skin.

Results: T-cadherin expression is restricted to basal keratinocytes of the epidermis. The basal cell layer of sebaceous glands was T-cadherin positive, whereas sebocytes were negative. Within apocrine glands, only myoepithelial cells were T-cadherin positive. In contrast, both the secretory coils and excretory ducts of eccrine glands were T-cadherin positive. In terminal hair follicles, the outer root sheath layers strongly expressed T-cadherin throughout different regions of the follicle, with the strongest immunoreactivity at the bulge and suprabulbar regions. T-cadherin and CK15 stem cell marker similarly localized within the bulge and suprabulbar region. T-cadherin and CD34 stem cell marker similarly localized at the suprabulbar level.

Conclusion: The specific patterns of T-cadherin expression in the epidermis and adnexal structures suggest an important guardian role in skin homeostasis.

No MeSH data available.