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T-Cadherin Expression in the Epidermis and Adnexal Structures of Normal Skin

View Article: PubMed Central - PubMed

ABSTRACT

Background: T-cadherin is an atypical glycosylphosphatidylinositol-anchored member of the cadherin superfamily of adhesion molecules. The role of T-cadherin in biology of the skin is poorly understood. Expression of T-cadherin in basal keratinocytes and dermal blood vessels of the healthy epidermis has been demonstrated, but studies on expression in skin appendages are rare.

Methods: We conducted an immunohistochemical analysis of T-cadherin expression in the epidermis and adnexal structures of normal skin.

Results: T-cadherin expression is restricted to basal keratinocytes of the epidermis. The basal cell layer of sebaceous glands was T-cadherin positive, whereas sebocytes were negative. Within apocrine glands, only myoepithelial cells were T-cadherin positive. In contrast, both the secretory coils and excretory ducts of eccrine glands were T-cadherin positive. In terminal hair follicles, the outer root sheath layers strongly expressed T-cadherin throughout different regions of the follicle, with the strongest immunoreactivity at the bulge and suprabulbar regions. T-cadherin and CK15 stem cell marker similarly localized within the bulge and suprabulbar region. T-cadherin and CD34 stem cell marker similarly localized at the suprabulbar level.

Conclusion: The specific patterns of T-cadherin expression in the epidermis and adnexal structures suggest an important guardian role in skin homeostasis.

No MeSH data available.


T-cadherin expression in normal epidermis. Note the strong staining in the basal keratinocyte layer, with a typically global distribution over the cell body and pronounced expression on the basal side and intercellular contacts of the epidermal basal cells (a). Close-up showing loss of T-cadherin expression in the suprabasal layers of epidermis (arrowheads; a). Strongly positive expression of T-cadherin on endothelial cells of dermal blood vessels is evident (a, b). Note the less intense staining for T-cadherin within the subendothelial smooth muscle cell layers in arteries (arrowheads; b).
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Figure 1: T-cadherin expression in normal epidermis. Note the strong staining in the basal keratinocyte layer, with a typically global distribution over the cell body and pronounced expression on the basal side and intercellular contacts of the epidermal basal cells (a). Close-up showing loss of T-cadherin expression in the suprabasal layers of epidermis (arrowheads; a). Strongly positive expression of T-cadherin on endothelial cells of dermal blood vessels is evident (a, b). Note the less intense staining for T-cadherin within the subendothelial smooth muscle cell layers in arteries (arrowheads; b).

Mentions: T-cadherin was strongly expressed in the basal keratinocyte layer, with a typically global distribution over the cell membrane and pronounced expression at intercellular junctions and on the basal and apical sides of the epidermal basal cells (Fig. 1a). There was a weak cell body and/or nuclear expression of T-cadherin on occasional suprabasal keratinocytes (Fig. 1a). Except for isolated cells, there was no T-cadherin expression in the upper spinous layer of the epidermis.


T-Cadherin Expression in the Epidermis and Adnexal Structures of Normal Skin
T-cadherin expression in normal epidermis. Note the strong staining in the basal keratinocyte layer, with a typically global distribution over the cell body and pronounced expression on the basal side and intercellular contacts of the epidermal basal cells (a). Close-up showing loss of T-cadherin expression in the suprabasal layers of epidermis (arrowheads; a). Strongly positive expression of T-cadherin on endothelial cells of dermal blood vessels is evident (a, b). Note the less intense staining for T-cadherin within the subendothelial smooth muscle cell layers in arteries (arrowheads; b).
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC5121566&req=5

Figure 1: T-cadherin expression in normal epidermis. Note the strong staining in the basal keratinocyte layer, with a typically global distribution over the cell body and pronounced expression on the basal side and intercellular contacts of the epidermal basal cells (a). Close-up showing loss of T-cadherin expression in the suprabasal layers of epidermis (arrowheads; a). Strongly positive expression of T-cadherin on endothelial cells of dermal blood vessels is evident (a, b). Note the less intense staining for T-cadherin within the subendothelial smooth muscle cell layers in arteries (arrowheads; b).
Mentions: T-cadherin was strongly expressed in the basal keratinocyte layer, with a typically global distribution over the cell membrane and pronounced expression at intercellular junctions and on the basal and apical sides of the epidermal basal cells (Fig. 1a). There was a weak cell body and/or nuclear expression of T-cadherin on occasional suprabasal keratinocytes (Fig. 1a). Except for isolated cells, there was no T-cadherin expression in the upper spinous layer of the epidermis.

View Article: PubMed Central - PubMed

ABSTRACT

Background: T-cadherin is an atypical glycosylphosphatidylinositol-anchored member of the cadherin superfamily of adhesion molecules. The role of T-cadherin in biology of the skin is poorly understood. Expression of T-cadherin in basal keratinocytes and dermal blood vessels of the healthy epidermis has been demonstrated, but studies on expression in skin appendages are rare.

Methods: We conducted an immunohistochemical analysis of T-cadherin expression in the epidermis and adnexal structures of normal skin.

Results: T-cadherin expression is restricted to basal keratinocytes of the epidermis. The basal cell layer of sebaceous glands was T-cadherin positive, whereas sebocytes were negative. Within apocrine glands, only myoepithelial cells were T-cadherin positive. In contrast, both the secretory coils and excretory ducts of eccrine glands were T-cadherin positive. In terminal hair follicles, the outer root sheath layers strongly expressed T-cadherin throughout different regions of the follicle, with the strongest immunoreactivity at the bulge and suprabulbar regions. T-cadherin and CK15 stem cell marker similarly localized within the bulge and suprabulbar region. T-cadherin and CD34 stem cell marker similarly localized at the suprabulbar level.

Conclusion: The specific patterns of T-cadherin expression in the epidermis and adnexal structures suggest an important guardian role in skin homeostasis.

No MeSH data available.