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Making standards for quantitative real-time pneumococcal PCR

View Article: PubMed Central - PubMed

ABSTRACT

Quantitative lytA PCR is often performed using in-house standards. We hypothesised equivalence when measuring a standard suspension of Streptococcus pneumoniae by colony-forming-units (CFU) or genome-copies. Median (IQR) ratio of CFU/genome-copies was 0.19 (0.1–1.2). Genome-copies were less variable than CFU, but the discrepancy between the methods highlights challenges with absolute quantification.

No MeSH data available.


Poor correlation between methods of measuring bacterial concentration: colony counting in CFU/mL and DNA concentration in genome-copies/mL.
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fig0005: Poor correlation between methods of measuring bacterial concentration: colony counting in CFU/mL and DNA concentration in genome-copies/mL.


Making standards for quantitative real-time pneumococcal PCR
Poor correlation between methods of measuring bacterial concentration: colony counting in CFU/mL and DNA concentration in genome-copies/mL.
© Copyright Policy - CC BY-NC-ND
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC5121202&req=5

fig0005: Poor correlation between methods of measuring bacterial concentration: colony counting in CFU/mL and DNA concentration in genome-copies/mL.

View Article: PubMed Central - PubMed

ABSTRACT

Quantitative lytA PCR is often performed using in-house standards. We hypothesised equivalence when measuring a standard suspension of Streptococcus pneumoniae by colony-forming-units (CFU) or genome-copies. Median (IQR) ratio of CFU/genome-copies was 0.19 (0.1–1.2). Genome-copies were less variable than CFU, but the discrepancy between the methods highlights challenges with absolute quantification.

No MeSH data available.