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Data on the uptake and metabolism of the vertebrate steroid estradiol-17 β from water by the common mussel, Mytilus spp.

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ABSTRACT

The data presented in this article primarily provide support for the research article entitled “Mussels (Mytilus spp.) display an ability for rapid and high capacity uptake of the vertebrate steroid, estradiol-17β from water” (T.I. Schwarz, I. Katsiadaki, B.H. Maskrey, A.P. Scott, 2016) [1]. Data are presented on the ability of mussels to absorb tritiated estradiol (E2) from water. The data indicate that most of the radioactivity remaining in the water is 1,3,5(10)-estratriene-3,17β-diol 3-sulfate (E2 3-S) and the radioactivity in the mussel tissue is mainly in the form of fatty acid esters. The latter, following saponification, were identified by ultra-high performance liquid chromatography in conjunction with tandem mass spectrometry (UHPLC-MS/MS) as intact E2. Data are included that indicate that the remaining radioactivity in the tissue is composed of E2 3-S and unidentified free metabolites. Experimental data included also relate to a) the efficiency of extraction of radioactivity from tissue, b) the efficiency of separation of free and esterified E2 using solvents and c) possible factors affecting the recovery of radioactivity. Finally, preliminary data are provided on concentrations of immunoreactive E2 in the free and ester fractions of tissue extracts from mussels caged in the field.

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Removal of radiolabel by Mytilus spp. during three separate 24 h exposures to [3H]-E2. Two of the exposures (Experiments 4 & 5; ○, □) were carried out in duplicate with aeration and 5 animals in 2 L water (they were set up as positive controls to examine the uptake of other steroids). The final exposure (Experiment 6; △) was carried out in a single vessel with 18 animals in 3.6 L water (and was set up for a subsequent depuration experiment). The lines represent the same data fitted to a three parameter hyperbolic decay equation.
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f0015: Removal of radiolabel by Mytilus spp. during three separate 24 h exposures to [3H]-E2. Two of the exposures (Experiments 4 & 5; ○, □) were carried out in duplicate with aeration and 5 animals in 2 L water (they were set up as positive controls to examine the uptake of other steroids). The final exposure (Experiment 6; △) was carried out in a single vessel with 18 animals in 3.6 L water (and was set up for a subsequent depuration experiment). The lines represent the same data fitted to a three parameter hyperbolic decay equation.

Mentions: The data presented in this article show the uptake of radiolabeled E2 ([3H]-E2) from water by mussels under different conditions (Fig. 1, Fig. 2, Fig. 3); the production of E2 metabolites in water (E2 3-S; Fig. 4) and tissue extracts (E2 3-S, Fig. 5, Fig. 6; E2 esters, Fig. 7), the identification of intact E2 in the ester fraction of saponified tissue extracts (Fig. 8) and the concentrations of free and esterified E2 in tissue extracts of mussels caged in the field (Fig. 9). Original data from experiments that were carried out to determine the best methods for extracting (Table 1) and then separating free and esterified E2 (Table 2) are also presented.


Data on the uptake and metabolism of the vertebrate steroid estradiol-17 β from water by the common mussel, Mytilus spp.
Removal of radiolabel by Mytilus spp. during three separate 24 h exposures to [3H]-E2. Two of the exposures (Experiments 4 & 5; ○, □) were carried out in duplicate with aeration and 5 animals in 2 L water (they were set up as positive controls to examine the uptake of other steroids). The final exposure (Experiment 6; △) was carried out in a single vessel with 18 animals in 3.6 L water (and was set up for a subsequent depuration experiment). The lines represent the same data fitted to a three parameter hyperbolic decay equation.
© Copyright Policy - CC BY
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC5121145&req=5

f0015: Removal of radiolabel by Mytilus spp. during three separate 24 h exposures to [3H]-E2. Two of the exposures (Experiments 4 & 5; ○, □) were carried out in duplicate with aeration and 5 animals in 2 L water (they were set up as positive controls to examine the uptake of other steroids). The final exposure (Experiment 6; △) was carried out in a single vessel with 18 animals in 3.6 L water (and was set up for a subsequent depuration experiment). The lines represent the same data fitted to a three parameter hyperbolic decay equation.
Mentions: The data presented in this article show the uptake of radiolabeled E2 ([3H]-E2) from water by mussels under different conditions (Fig. 1, Fig. 2, Fig. 3); the production of E2 metabolites in water (E2 3-S; Fig. 4) and tissue extracts (E2 3-S, Fig. 5, Fig. 6; E2 esters, Fig. 7), the identification of intact E2 in the ester fraction of saponified tissue extracts (Fig. 8) and the concentrations of free and esterified E2 in tissue extracts of mussels caged in the field (Fig. 9). Original data from experiments that were carried out to determine the best methods for extracting (Table 1) and then separating free and esterified E2 (Table 2) are also presented.

View Article: PubMed Central - PubMed

ABSTRACT

The data presented in this article primarily provide support for the research article entitled “Mussels (Mytilus spp.) display an ability for rapid and high capacity uptake of the vertebrate steroid, estradiol-17β from water” (T.I. Schwarz, I. Katsiadaki, B.H. Maskrey, A.P. Scott, 2016) [1]. Data are presented on the ability of mussels to absorb tritiated estradiol (E2) from water. The data indicate that most of the radioactivity remaining in the water is 1,3,5(10)-estratriene-3,17β-diol 3-sulfate (E2 3-S) and the radioactivity in the mussel tissue is mainly in the form of fatty acid esters. The latter, following saponification, were identified by ultra-high performance liquid chromatography in conjunction with tandem mass spectrometry (UHPLC-MS/MS) as intact E2. Data are included that indicate that the remaining radioactivity in the tissue is composed of E2 3-S and unidentified free metabolites. Experimental data included also relate to a) the efficiency of extraction of radioactivity from tissue, b) the efficiency of separation of free and esterified E2 using solvents and c) possible factors affecting the recovery of radioactivity. Finally, preliminary data are provided on concentrations of immunoreactive E2 in the free and ester fractions of tissue extracts from mussels caged in the field.

No MeSH data available.


Related in: MedlinePlus