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Dpp dependent Hematopoietic stem cells give rise to Hh dependent blood progenitors in larval lymph gland of Drosophila

View Article: PubMed Central - PubMed

ABSTRACT

Drosophila hematopoiesis bears striking resemblance with that of vertebrates, both in the context of distinct phases and the signaling molecules. Even though, there has been no evidence of Hematopoietic stem cells (HSCs) in Drosophila, the larval lymph gland with its Hedgehog dependent progenitors served as an invertebrate model of progenitor biology. Employing lineage-tracing analyses, we have now identified Notch expressing HSCs in the first instar larval lymph gland. Our studies clearly establish the hierarchical relationship between Notch expressing HSCs and the previously described Domeless expressing progenitors. These HSCs require Decapentapelagic (Dpp) signal from the hematopoietic niche for their maintenance in an identical manner to vertebrate aorta-gonadal-mesonephros (AGM) HSCs. Thus, this study not only extends the conservation across these divergent taxa, but also provides a new model that can be exploited to gain better insight into the AGM related Hematopoietic stem cells (HSCs).

Doi:: http://dx.doi.org/10.7554/eLife.18295.001

No MeSH data available.


Related in: MedlinePlus

HSCs and progenitor lineages in the lymph gland.A scheme showing fate maps of progenitor and N expressing HSCs. (A) Progenitor cells give rise to all cells of the lymph gland. (B) N expressing cells are founder cells of progenitors, thus can also give rise to majority of the cells of the lymph gland through an intermediate step of progenitor generation. N expressing HSCs are higher in order to dome expressing progenitors. All cells are color coded according to G-TRACE construct (red: live; green: lineage traced; yellow: live and lineage traced). DV: dorsal vessel; Pro: progenitors; HSC: N expressing cells.DOI:http://dx.doi.org/10.7554/eLife.18295.020
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fig6s1: HSCs and progenitor lineages in the lymph gland.A scheme showing fate maps of progenitor and N expressing HSCs. (A) Progenitor cells give rise to all cells of the lymph gland. (B) N expressing cells are founder cells of progenitors, thus can also give rise to majority of the cells of the lymph gland through an intermediate step of progenitor generation. N expressing HSCs are higher in order to dome expressing progenitors. All cells are color coded according to G-TRACE construct (red: live; green: lineage traced; yellow: live and lineage traced). DV: dorsal vessel; Pro: progenitors; HSC: N expressing cells.DOI:http://dx.doi.org/10.7554/eLife.18295.020

Mentions: Put together, we can conclude that the Notch positive cells are higher in the order to Dome expressing progenitors and that the transient Notch expressing cells of first instar lymph gland are the multi-potent founder cells or the HSCs (Figure 6—figure supplement 1).


Dpp dependent Hematopoietic stem cells give rise to Hh dependent blood progenitors in larval lymph gland of Drosophila
HSCs and progenitor lineages in the lymph gland.A scheme showing fate maps of progenitor and N expressing HSCs. (A) Progenitor cells give rise to all cells of the lymph gland. (B) N expressing cells are founder cells of progenitors, thus can also give rise to majority of the cells of the lymph gland through an intermediate step of progenitor generation. N expressing HSCs are higher in order to dome expressing progenitors. All cells are color coded according to G-TRACE construct (red: live; green: lineage traced; yellow: live and lineage traced). DV: dorsal vessel; Pro: progenitors; HSC: N expressing cells.DOI:http://dx.doi.org/10.7554/eLife.18295.020
© Copyright Policy
Related In: Results  -  Collection

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Show All Figures
getmorefigures.php?uid=PMC5120881&req=5

fig6s1: HSCs and progenitor lineages in the lymph gland.A scheme showing fate maps of progenitor and N expressing HSCs. (A) Progenitor cells give rise to all cells of the lymph gland. (B) N expressing cells are founder cells of progenitors, thus can also give rise to majority of the cells of the lymph gland through an intermediate step of progenitor generation. N expressing HSCs are higher in order to dome expressing progenitors. All cells are color coded according to G-TRACE construct (red: live; green: lineage traced; yellow: live and lineage traced). DV: dorsal vessel; Pro: progenitors; HSC: N expressing cells.DOI:http://dx.doi.org/10.7554/eLife.18295.020
Mentions: Put together, we can conclude that the Notch positive cells are higher in the order to Dome expressing progenitors and that the transient Notch expressing cells of first instar lymph gland are the multi-potent founder cells or the HSCs (Figure 6—figure supplement 1).

View Article: PubMed Central - PubMed

ABSTRACT

Drosophila hematopoiesis bears striking resemblance with that of vertebrates, both in the context of distinct phases and the signaling molecules. Even though, there has been no evidence of Hematopoietic stem cells (HSCs) in Drosophila, the larval lymph gland with its Hedgehog dependent progenitors served as an invertebrate model of progenitor biology. Employing lineage-tracing analyses, we have now identified Notch expressing HSCs in the first instar larval lymph gland. Our studies clearly establish the hierarchical relationship between Notch expressing HSCs and the previously described Domeless expressing progenitors. These HSCs require Decapentapelagic (Dpp) signal from the hematopoietic niche for their maintenance in an identical manner to vertebrate aorta-gonadal-mesonephros (AGM) HSCs. Thus, this study not only extends the conservation across these divergent taxa, but also provides a new model that can be exploited to gain better insight into the AGM related Hematopoietic stem cells (HSCs).

Doi:: http://dx.doi.org/10.7554/eLife.18295.001

No MeSH data available.


Related in: MedlinePlus