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Dpp dependent Hematopoietic stem cells give rise to Hh dependent blood progenitors in larval lymph gland of Drosophila

View Article: PubMed Central - PubMed

ABSTRACT

Drosophila hematopoiesis bears striking resemblance with that of vertebrates, both in the context of distinct phases and the signaling molecules. Even though, there has been no evidence of Hematopoietic stem cells (HSCs) in Drosophila, the larval lymph gland with its Hedgehog dependent progenitors served as an invertebrate model of progenitor biology. Employing lineage-tracing analyses, we have now identified Notch expressing HSCs in the first instar larval lymph gland. Our studies clearly establish the hierarchical relationship between Notch expressing HSCs and the previously described Domeless expressing progenitors. These HSCs require Decapentapelagic (Dpp) signal from the hematopoietic niche for their maintenance in an identical manner to vertebrate aorta-gonadal-mesonephros (AGM) HSCs. Thus, this study not only extends the conservation across these divergent taxa, but also provides a new model that can be exploited to gain better insight into the AGM related Hematopoietic stem cells (HSCs).

Doi:: http://dx.doi.org/10.7554/eLife.18295.001

No MeSH data available.


Related in: MedlinePlus

Fate map of the progenitors arising from Notch expressing HSCs.(A) shows dome-GFP expression in progenitors of a late third instar lymph gland (n = 10). (B–C") show that dome expressing progenitors upon lineage tracing (C–C', green) from 18 hr AEH using the scheme in (B) give rise to all the cells of the lymph gland. Red indicates current expression of dome whereas green shows lineage of the same (C–C"; n = 10). (D–E"') show that dome lineage comprises of both crystal cells (D'–D"', Hnt, grey; n = 13) and plasmatocytes (E–E"', P1, grey; n = 15). Genotypes are shown on top of corresponding panels. Scale bar = 20 μm. DAPI marks the nucleus. Hours after larval hatching are as indicated in each panel.DOI:http://dx.doi.org/10.7554/eLife.18295.013
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fig3s1: Fate map of the progenitors arising from Notch expressing HSCs.(A) shows dome-GFP expression in progenitors of a late third instar lymph gland (n = 10). (B–C") show that dome expressing progenitors upon lineage tracing (C–C', green) from 18 hr AEH using the scheme in (B) give rise to all the cells of the lymph gland. Red indicates current expression of dome whereas green shows lineage of the same (C–C"; n = 10). (D–E"') show that dome lineage comprises of both crystal cells (D'–D"', Hnt, grey; n = 13) and plasmatocytes (E–E"', P1, grey; n = 15). Genotypes are shown on top of corresponding panels. Scale bar = 20 μm. DAPI marks the nucleus. Hours after larval hatching are as indicated in each panel.DOI:http://dx.doi.org/10.7554/eLife.18295.013

Mentions: Activation of the construct post 18 hr AEH by dome-Gal4 (Figure 3—figure supplement 1A–C"), resulted in labeling of all cells of the lymph gland (Figure 3—figure supplement 1D–D"', Hindsight, Hnt: Crystal cells (Terriente-Felix et al., 2013) and Figure 3—figure supplement 1E–E"', P1: Plasmatocytes (Kurucz et al., 2007) indicating that they are multipotent in nature.


Dpp dependent Hematopoietic stem cells give rise to Hh dependent blood progenitors in larval lymph gland of Drosophila
Fate map of the progenitors arising from Notch expressing HSCs.(A) shows dome-GFP expression in progenitors of a late third instar lymph gland (n = 10). (B–C") show that dome expressing progenitors upon lineage tracing (C–C', green) from 18 hr AEH using the scheme in (B) give rise to all the cells of the lymph gland. Red indicates current expression of dome whereas green shows lineage of the same (C–C"; n = 10). (D–E"') show that dome lineage comprises of both crystal cells (D'–D"', Hnt, grey; n = 13) and plasmatocytes (E–E"', P1, grey; n = 15). Genotypes are shown on top of corresponding panels. Scale bar = 20 μm. DAPI marks the nucleus. Hours after larval hatching are as indicated in each panel.DOI:http://dx.doi.org/10.7554/eLife.18295.013
© Copyright Policy
Related In: Results  -  Collection

License
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getmorefigures.php?uid=PMC5120881&req=5

fig3s1: Fate map of the progenitors arising from Notch expressing HSCs.(A) shows dome-GFP expression in progenitors of a late third instar lymph gland (n = 10). (B–C") show that dome expressing progenitors upon lineage tracing (C–C', green) from 18 hr AEH using the scheme in (B) give rise to all the cells of the lymph gland. Red indicates current expression of dome whereas green shows lineage of the same (C–C"; n = 10). (D–E"') show that dome lineage comprises of both crystal cells (D'–D"', Hnt, grey; n = 13) and plasmatocytes (E–E"', P1, grey; n = 15). Genotypes are shown on top of corresponding panels. Scale bar = 20 μm. DAPI marks the nucleus. Hours after larval hatching are as indicated in each panel.DOI:http://dx.doi.org/10.7554/eLife.18295.013
Mentions: Activation of the construct post 18 hr AEH by dome-Gal4 (Figure 3—figure supplement 1A–C"), resulted in labeling of all cells of the lymph gland (Figure 3—figure supplement 1D–D"', Hindsight, Hnt: Crystal cells (Terriente-Felix et al., 2013) and Figure 3—figure supplement 1E–E"', P1: Plasmatocytes (Kurucz et al., 2007) indicating that they are multipotent in nature.

View Article: PubMed Central - PubMed

ABSTRACT

Drosophila hematopoiesis bears striking resemblance with that of vertebrates, both in the context of distinct phases and the signaling molecules. Even though, there has been no evidence of Hematopoietic stem cells (HSCs) in Drosophila, the larval lymph gland with its Hedgehog dependent progenitors served as an invertebrate model of progenitor biology. Employing lineage-tracing analyses, we have now identified Notch expressing HSCs in the first instar larval lymph gland. Our studies clearly establish the hierarchical relationship between Notch expressing HSCs and the previously described Domeless expressing progenitors. These HSCs require Decapentapelagic (Dpp) signal from the hematopoietic niche for their maintenance in an identical manner to vertebrate aorta-gonadal-mesonephros (AGM) HSCs. Thus, this study not only extends the conservation across these divergent taxa, but also provides a new model that can be exploited to gain better insight into the AGM related Hematopoietic stem cells (HSCs).

Doi:: http://dx.doi.org/10.7554/eLife.18295.001

No MeSH data available.


Related in: MedlinePlus