Limits...
Treatment of Oral Biofilms by a D-Enantiomeric Peptide

View Article: PubMed Central - PubMed

ABSTRACT

Almost all dental diseases are caused by biofilms that consist of multispecies communities. DJK-5, which is a short D-enantiomeric, protease-resistant peptide with broad-spectrum anti-biofilm activity, was tested for its effect on oral multispecies biofilms. Peptide DJK-5 at 10 μg/mL effectively prevented the growth of these microbes in culture media in a time-dependent manner. In addition to the prevention of growth, peptide DJK-5 completely killed both Streptococcus mutans and Enterococcus faecalis suspended from biofilms after 30 minutes of incubation in liquid culture media. DJK-5 also led to the effective killing of microbes in plaque biofilm. The proportion of bacterial cells killed by 10 μg/mL of DJK-5 was similar after 1 and 3 days, both exceeding 85%. DJK-5 was able to significantly prevent biofilm formation over 3 days (P = 0.000). After 72 hours of exposure, DJK-5 significantly reduced and almost completely prevented plaque biofilm production by more than 90% of biovolume compared to untreated controls (P = 0.000). The proportion of dead biofilm bacteria at the 10 μg/mL DJK-5 concentration was similar for 1- and 3-day-old biofilms, whereby >86% of the bacteria were killed. DJK-5 was also able to kill >79% and >85% of bacteria, respectively, after one-time and three brief treatments of 3-day-old biofilms. The combination of DJK-5 and chlorhexidine showed the best bacterial killing among all treatments, with ~83% and >88% of bacterial cells killed after 1 and 3 minutes, respectively. No significant difference was found in the percentage of biofilm killing amongst three donor plaque samples after DJK-5 treatment. In particular, DJK-5 showed strong performance in inhibiting biofilm development and eradicating pre-formed oral biofilms compared to L-enantiomeric peptide 1018. DJK-5 was very effective against oral biofilms when used alone or combined with chlorhexidine, and may be a promising agent for use in oral anti-biofilm strategies in the future.

No MeSH data available.


Related in: MedlinePlus

Superior killing of plaque biofilm microbes by peptide DJK-5 (cf. 1018) with chlorhexidine when used in combination for 1 or 3 minutes.(A-C) 3D CLSM images; the scale bar represents 200 μm. (D) The proportion of dead microbial cells after treatment with peptide, CHX, or the combination of both agents. Data calculated from A-C. Error bars represent the standard deviations calculated from three independent experiments.
© Copyright Policy
Related In: Results  -  Collection

License
getmorefigures.php?uid=PMC5120842&req=5

pone.0166997.g005: Superior killing of plaque biofilm microbes by peptide DJK-5 (cf. 1018) with chlorhexidine when used in combination for 1 or 3 minutes.(A-C) 3D CLSM images; the scale bar represents 200 μm. (D) The proportion of dead microbial cells after treatment with peptide, CHX, or the combination of both agents. Data calculated from A-C. Error bars represent the standard deviations calculated from three independent experiments.

Mentions: The combination of DJK-5 and CHX showed the best results in bacterial killing among all groups. The combination killed 83–87% and 88–90% of the bacteria after just 1 and 3 minutes, respectively (Fig 5; P = 0.000). DJK-5 alone killed a much higher percentage of bacteria than the combination of 1018 and CHX, or either CHX or peptide 1018 alone (P < 0.01).


Treatment of Oral Biofilms by a D-Enantiomeric Peptide
Superior killing of plaque biofilm microbes by peptide DJK-5 (cf. 1018) with chlorhexidine when used in combination for 1 or 3 minutes.(A-C) 3D CLSM images; the scale bar represents 200 μm. (D) The proportion of dead microbial cells after treatment with peptide, CHX, or the combination of both agents. Data calculated from A-C. Error bars represent the standard deviations calculated from three independent experiments.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC5120842&req=5

pone.0166997.g005: Superior killing of plaque biofilm microbes by peptide DJK-5 (cf. 1018) with chlorhexidine when used in combination for 1 or 3 minutes.(A-C) 3D CLSM images; the scale bar represents 200 μm. (D) The proportion of dead microbial cells after treatment with peptide, CHX, or the combination of both agents. Data calculated from A-C. Error bars represent the standard deviations calculated from three independent experiments.
Mentions: The combination of DJK-5 and CHX showed the best results in bacterial killing among all groups. The combination killed 83–87% and 88–90% of the bacteria after just 1 and 3 minutes, respectively (Fig 5; P = 0.000). DJK-5 alone killed a much higher percentage of bacteria than the combination of 1018 and CHX, or either CHX or peptide 1018 alone (P < 0.01).

View Article: PubMed Central - PubMed

ABSTRACT

Almost all dental diseases are caused by biofilms that consist of multispecies communities. DJK-5, which is a short D-enantiomeric, protease-resistant peptide with broad-spectrum anti-biofilm activity, was tested for its effect on oral multispecies biofilms. Peptide DJK-5 at 10 &mu;g/mL effectively prevented the growth of these microbes in culture media in a time-dependent manner. In addition to the prevention of growth, peptide DJK-5 completely killed both Streptococcus mutans and Enterococcus faecalis suspended from biofilms after 30 minutes of incubation in liquid culture media. DJK-5 also led to the effective killing of microbes in plaque biofilm. The proportion of bacterial cells killed by 10 &mu;g/mL of DJK-5 was similar after 1 and 3 days, both exceeding 85%. DJK-5 was able to significantly prevent biofilm formation over 3 days (P = 0.000). After 72 hours of exposure, DJK-5 significantly reduced and almost completely prevented plaque biofilm production by more than 90% of biovolume compared to untreated controls (P = 0.000). The proportion of dead biofilm bacteria at the 10 &mu;g/mL DJK-5 concentration was similar for 1- and 3-day-old biofilms, whereby &gt;86% of the bacteria were killed. DJK-5 was also able to kill &gt;79% and &gt;85% of bacteria, respectively, after one-time and three brief treatments of 3-day-old biofilms. The combination of DJK-5 and chlorhexidine showed the best bacterial killing among all treatments, with ~83% and &gt;88% of bacterial cells killed after 1 and 3 minutes, respectively. No significant difference was found in the percentage of biofilm killing amongst three donor plaque samples after DJK-5 treatment. In particular, DJK-5 showed strong performance in inhibiting biofilm development and eradicating pre-formed oral biofilms compared to L-enantiomeric peptide 1018. DJK-5 was very effective against oral biofilms when used alone or combined with chlorhexidine, and may be a promising agent for use in oral anti-biofilm strategies in the future.

No MeSH data available.


Related in: MedlinePlus