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Microvascular Blood Flow Improvement in Hyperglycemic Obese Adult Patients by Hypocaloric Diet

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ABSTRACT

The present study was aimed to assess the changes in skin microvascular blood flow (SBF) in newly diagnosed hyperglycemic obese subjects, administered with hypocaloric diet. Adult patients were recruited and divided in three groups: NW group (n=54), NG (n=54) and HG (n=54) groups were constituted by normal weight, normoglycemic and hyperglycemic obese subjects, respectively. SBF was measured by laser Doppler perfusion monitoring technique and oscillations in blood flow were analyzed by spectral methods under baseline conditions, at 3 and 6 months of dietary treatment. Under resting conditions, SBF was lower in HG group than in NG and NW ones. Moreover, all subjects showed blood flow oscillations with several frequency components. In particular, hyperglycemic obese patients revealed lower spectral density in myogenic-related component than normoglycemic obese and normal weight ones. Moreover, post-occlusive reactive hyperemia (PORH) was impaired in hyperglycemic obese compared to normoglycemic and normal weigh subjects. After hypocaloric diet, in hyperglycemic obese patients there was an improvement in SBF accompanied by recovery in myogenic-related oscillations and arteriolar responses during PORH. In conclusion, hyperglycemia markedly affected peripheral microvascular function; hypocaloric diet ameliorated tissue blood flow.

No MeSH data available.


Related in: MedlinePlus

Normalized power spectral density, expressed as percent, related to NO-independent endothelial activity (1), NO-dependent endothelial activity (2), neurogenic activity (3), myogenic activity (4), respiration (5) and heart rate (6) under baseline conditions (T0) in normal weight (NW), normoglycemic (NG) and hyperglycemic patients (HG). ∘ p< 0.01 vs T0 NW group, * p< 0.01 vs T0 NG group.
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f2-tm-15-01: Normalized power spectral density, expressed as percent, related to NO-independent endothelial activity (1), NO-dependent endothelial activity (2), neurogenic activity (3), myogenic activity (4), respiration (5) and heart rate (6) under baseline conditions (T0) in normal weight (NW), normoglycemic (NG) and hyperglycemic patients (HG). ∘ p< 0.01 vs T0 NW group, * p< 0.01 vs T0 NG group.

Mentions: Under baseline conditions, in all LDPM tracings, several frequency components were detected; four in low frequency component ranges: 0.005–0.0095 Hz, 0.0095–0.021 Hz, 0.021–0.052 Hz and 0.052–0.15 Hz. These frequency components were related to the NO-independent and NO-dependent endothelium activity, neurogenic and myogenic activities, respectively. Other two high frequency components were detected in the ranges: 0.15–0.6 Hz and 0.6–2.0 Hz, related to the respiratory and heart rates, respectively. The power spectral density of neurogenic and myogenic activities were higher than those related to the other parameters, as observed in normal weight subjects (Figure 2). However, in hyperglycemic patients the PSD related to myogenic activity (in percentage of total spectral density) was significantly lower than in normoglycemic obese and normal weight subjects (30.1 ± 0.7 vs 34.9 ± 0.7 and 38.3 ± 0.6 %, p<0.01 vs NG and NW groups; Figure 2). On the other hand, percent NO-dependent spectral density was higher (10.3 ± 0.7 vs 7.9 ± 0.3 %, p<0.01 vs NG) in hyperglycemic than in normoglycemic obese patients, but there was no significant difference when compared with the values evaluated in normal weight subjects (9.8 ± 1.0 %; Figure 2). The changes in the low frequency component spectral density were accompanied by the corresponding variations in high frequency components: respiration-related spectral density was higher in hyperglycemic than in normoglycemic and normal weight persons (16.5 ± 0.8 vs 10.1 ± 0.6 and 9.7 ± 0.5 %, p<0.01 vs NG and NW groups), while hearth rate related spectral density was the lowest in normal weight subjects (3.1 ± 0.2 vs 10.5 ± 1.0 and 6.8 ± 0.4 %, p<0.01 vs NG and HG groups) (Figure 2).


Microvascular Blood Flow Improvement in Hyperglycemic Obese Adult Patients by Hypocaloric Diet
Normalized power spectral density, expressed as percent, related to NO-independent endothelial activity (1), NO-dependent endothelial activity (2), neurogenic activity (3), myogenic activity (4), respiration (5) and heart rate (6) under baseline conditions (T0) in normal weight (NW), normoglycemic (NG) and hyperglycemic patients (HG). ∘ p< 0.01 vs T0 NW group, * p< 0.01 vs T0 NG group.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC5120744&req=5

f2-tm-15-01: Normalized power spectral density, expressed as percent, related to NO-independent endothelial activity (1), NO-dependent endothelial activity (2), neurogenic activity (3), myogenic activity (4), respiration (5) and heart rate (6) under baseline conditions (T0) in normal weight (NW), normoglycemic (NG) and hyperglycemic patients (HG). ∘ p< 0.01 vs T0 NW group, * p< 0.01 vs T0 NG group.
Mentions: Under baseline conditions, in all LDPM tracings, several frequency components were detected; four in low frequency component ranges: 0.005–0.0095 Hz, 0.0095–0.021 Hz, 0.021–0.052 Hz and 0.052–0.15 Hz. These frequency components were related to the NO-independent and NO-dependent endothelium activity, neurogenic and myogenic activities, respectively. Other two high frequency components were detected in the ranges: 0.15–0.6 Hz and 0.6–2.0 Hz, related to the respiratory and heart rates, respectively. The power spectral density of neurogenic and myogenic activities were higher than those related to the other parameters, as observed in normal weight subjects (Figure 2). However, in hyperglycemic patients the PSD related to myogenic activity (in percentage of total spectral density) was significantly lower than in normoglycemic obese and normal weight subjects (30.1 ± 0.7 vs 34.9 ± 0.7 and 38.3 ± 0.6 %, p<0.01 vs NG and NW groups; Figure 2). On the other hand, percent NO-dependent spectral density was higher (10.3 ± 0.7 vs 7.9 ± 0.3 %, p<0.01 vs NG) in hyperglycemic than in normoglycemic obese patients, but there was no significant difference when compared with the values evaluated in normal weight subjects (9.8 ± 1.0 %; Figure 2). The changes in the low frequency component spectral density were accompanied by the corresponding variations in high frequency components: respiration-related spectral density was higher in hyperglycemic than in normoglycemic and normal weight persons (16.5 ± 0.8 vs 10.1 ± 0.6 and 9.7 ± 0.5 %, p<0.01 vs NG and NW groups), while hearth rate related spectral density was the lowest in normal weight subjects (3.1 ± 0.2 vs 10.5 ± 1.0 and 6.8 ± 0.4 %, p<0.01 vs NG and HG groups) (Figure 2).

View Article: PubMed Central - PubMed

ABSTRACT

The present study was aimed to assess the changes in skin microvascular blood flow (SBF) in newly diagnosed hyperglycemic obese subjects, administered with hypocaloric diet. Adult patients were recruited and divided in three groups: NW group (n=54), NG (n=54) and HG (n=54) groups were constituted by normal weight, normoglycemic and hyperglycemic obese subjects, respectively. SBF was measured by laser Doppler perfusion monitoring technique and oscillations in blood flow were analyzed by spectral methods under baseline conditions, at 3 and 6 months of dietary treatment. Under resting conditions, SBF was lower in HG group than in NG and NW ones. Moreover, all subjects showed blood flow oscillations with several frequency components. In particular, hyperglycemic obese patients revealed lower spectral density in myogenic-related component than normoglycemic obese and normal weight ones. Moreover, post-occlusive reactive hyperemia (PORH) was impaired in hyperglycemic obese compared to normoglycemic and normal weigh subjects. After hypocaloric diet, in hyperglycemic obese patients there was an improvement in SBF accompanied by recovery in myogenic-related oscillations and arteriolar responses during PORH. In conclusion, hyperglycemia markedly affected peripheral microvascular function; hypocaloric diet ameliorated tissue blood flow.

No MeSH data available.


Related in: MedlinePlus