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Comparison of repair of peripheral nerve transection in predegenerated muscle with and without a vein graft

View Article: PubMed Central - PubMed

ABSTRACT

Background: Despite substantial research into the topic and valiant surgical efforts, reconstruction of peripheral nerve injury remains a challenging surgery. This study was conducted to evaluate the effectiveness of axonal regeneration of a transected sciatic nerve through a vein conduit containing degenerated skeletal muscle compared with axonal regeneration in a transected sciatic nerve through degenerated skeletal muscle alone.

Methods: In two of the three experimental rat groups, 10 mm of the left sciatic nerve was transected and removed. The proximal and distal ends of the transected sciatic nerve were then approximated and surrounded with either (a) a degenerated skeletal muscle graft; or (b) a graft containing both degenerated skeletal muscle and vein. In the group receiving the combined vein and skeletal muscle graft, the vein walls were subsequently sutured to the proximal and distal nerve stump epineurium. Sciatic functional index (SFI) was used for assessment of functional recovery. Tracing study and histological procedures were used to assess axonal regeneration.

Results: At 60 days, the gait functional recovery as well as the mean number of myelinated axons in the middle and distal parts of the sciatic nerve significantly increased in the group with the vein graft compared to rats with only the muscular graft (P < 0.05). Mean diameter of myelinated nerve fiber of the distal sciatic nerve was also improved with the vein graft compared to the muscle graft alone (P < 0.05). The mean number of DiI-labeled motor neurons in the L4-L5 spinal segment increased in the vein with muscle group but was not significantly different between the two groups.

Conclusions: These findings demonstrated that a graft consisting of not only predegenerated muscle, but also predegenerated muscle with vein more effectively supported nerve regeneration, thus promoting functional recovery after sciatic nerve injury in rats.

No MeSH data available.


Related in: MedlinePlus

Vein with muscle group significantly improved the functional recovery of the injured sciatic nerve. Walking track analysis (a), of the rat prints on the injured left side at 28 and 60 days after treatment. Sciatic functional index (SFI) values are expressed as mean ± SD, n = 12, *p < 0.001 compared to the vein with muscle and the muscle only graft groups, **p < 0.05 compared to the muscle only graft group (b)
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Fig1: Vein with muscle group significantly improved the functional recovery of the injured sciatic nerve. Walking track analysis (a), of the rat prints on the injured left side at 28 and 60 days after treatment. Sciatic functional index (SFI) values are expressed as mean ± SD, n = 12, *p < 0.001 compared to the vein with muscle and the muscle only graft groups, **p < 0.05 compared to the muscle only graft group (b)

Mentions: Photographs of the rat prints demonstrated the functional recovery improved from 28 toward 60 days post treatment in the both grafted groups (Fig. 1a). On the day before the surgery, the functional assessment based on SFI was carried out for each group. Moreover, no significant difference between groups and the result was reported. However, SFI was found to be greatly decreased at postoperative day seven in both the experiment group which received the vein with muscle graft and in the experimental group which received the muscle graft alone, especially compared to the sham group (P < 0.001), (Fig. 1b). Differences in mean SFI values were not significant between the group with the vein and muscle graft compared to the group with the muscle only graft from the first week postoperatively until the end of the fifth week. Yet 60 days after treatment, the gait functional recovery improved in the vein with muscle graft group (−74.6 ± 6.9) compared to the muscle only graft group (−96.9 ± 2.1) of rats (P < 0.05), (Fig. 1b).Fig. 1


Comparison of repair of peripheral nerve transection in predegenerated muscle with and without a vein graft
Vein with muscle group significantly improved the functional recovery of the injured sciatic nerve. Walking track analysis (a), of the rat prints on the injured left side at 28 and 60 days after treatment. Sciatic functional index (SFI) values are expressed as mean ± SD, n = 12, *p < 0.001 compared to the vein with muscle and the muscle only graft groups, **p < 0.05 compared to the muscle only graft group (b)
© Copyright Policy - OpenAccess
Related In: Results  -  Collection

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Fig1: Vein with muscle group significantly improved the functional recovery of the injured sciatic nerve. Walking track analysis (a), of the rat prints on the injured left side at 28 and 60 days after treatment. Sciatic functional index (SFI) values are expressed as mean ± SD, n = 12, *p < 0.001 compared to the vein with muscle and the muscle only graft groups, **p < 0.05 compared to the muscle only graft group (b)
Mentions: Photographs of the rat prints demonstrated the functional recovery improved from 28 toward 60 days post treatment in the both grafted groups (Fig. 1a). On the day before the surgery, the functional assessment based on SFI was carried out for each group. Moreover, no significant difference between groups and the result was reported. However, SFI was found to be greatly decreased at postoperative day seven in both the experiment group which received the vein with muscle graft and in the experimental group which received the muscle graft alone, especially compared to the sham group (P < 0.001), (Fig. 1b). Differences in mean SFI values were not significant between the group with the vein and muscle graft compared to the group with the muscle only graft from the first week postoperatively until the end of the fifth week. Yet 60 days after treatment, the gait functional recovery improved in the vein with muscle graft group (−74.6 ± 6.9) compared to the muscle only graft group (−96.9 ± 2.1) of rats (P < 0.05), (Fig. 1b).Fig. 1

View Article: PubMed Central - PubMed

ABSTRACT

Background: Despite substantial research into the topic and valiant surgical efforts, reconstruction of peripheral nerve injury remains a challenging surgery. This study was conducted to evaluate the effectiveness of axonal regeneration of a transected sciatic nerve through a vein conduit containing degenerated skeletal muscle compared with axonal regeneration in a transected sciatic nerve through degenerated skeletal muscle alone.

Methods: In two of the three experimental rat groups, 10&nbsp;mm of the left sciatic nerve was transected and removed. The proximal and distal ends of the transected sciatic nerve were then approximated and surrounded with either (a) a degenerated skeletal muscle graft; or (b) a graft containing both degenerated skeletal muscle and vein. In the group receiving the combined vein and skeletal muscle graft, the vein walls were subsequently sutured to the proximal and distal nerve stump epineurium. Sciatic functional index (SFI) was used for assessment of functional recovery. Tracing study and histological procedures were used to assess axonal regeneration.

Results: At 60&nbsp;days, the gait functional recovery as well as the mean number of myelinated axons in the middle and distal parts of the sciatic nerve significantly increased in the group with the vein graft compared to rats with only the muscular graft (P&thinsp;&lt;&thinsp;0.05). Mean diameter of myelinated nerve fiber of the distal sciatic nerve was also improved with the vein graft compared to the muscle graft alone (P&thinsp;&lt;&thinsp;0.05). The mean number of DiI-labeled motor neurons in the L4-L5 spinal segment increased in the vein with muscle group but was not significantly different between the two groups.

Conclusions: These findings demonstrated that a graft consisting of not only predegenerated muscle, but also predegenerated muscle with vein more effectively supported nerve regeneration, thus promoting functional recovery after sciatic nerve injury in rats.

No MeSH data available.


Related in: MedlinePlus