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Comparative analysis of four terpenoids in root and cortex of Tripterygium wilfordii Radix by different drying methods

View Article: PubMed Central - PubMed

ABSTRACT

Background: Tripterygium wilfordii Radix, a well-known traditional medicine in china which is used for treatment of inflammation, pain, tumor and immune regulation for centuries in china, accompany with the serious toxic side effects. This study was carried out for simultaneously analyzing the four main components (triptolide, triptophenolide, demethylzeylasteral and celastrol) in Tripterygium wilfordii Radix under different drying processes, which was important for reducing the toxicity and quality control of Tripterygium wilfordii Radix in future.

Methods: The terpenes were extracted by using ultrasonic method with ethyl acetate from root or cortex of Tripterygium wilfordii Radix, and the sensitive and rapid HPLC-PDA method was developed for simultaneous quantification of triptolide, triptophenolide, demethylzeylasteral and celastrol in root and cortex of Tripterygium wilfordii Radix for evaluation of the impacts by different drying processes.

Results: The four compounds in their respective determined arrange had good linearity of 0.9998≦R2≦0.9999 and the average recoveries were range from 94.69 to 100.28%, RSDs were within 0.27 to 2.42%, respectively. The contents of triptolide, triptophenolide, demethylzeylasteral and celastrol in different Tripterygium wilfordii Radix individuals were varied greatly at different drying temperatures. Under different temperatures, the contents of triptolide, triptophenolide, demethylzeylasteral, and celastrol were 37.94–70.31 mg/g, 0–1.807 mg/g, 0.3513–9.205 mg/g, 3.202–15.31 mg/g, respectively. The suitable drying temperature of terpenoids in root of wild and cultivate are 80 °C and 60 °C, the suitable drying temperature of terpenoids in cortex is 40 °C.

Conclusions: The method established is high sensitivity, accuracy, reliability and suitable for the simultaneous analysis of terpenoids in Tripterygium wilfordii Radix. The data provide a scientific basis and reference for the quality control of herb and preparations related to Tripterygium wilfordii Radix.

No MeSH data available.


The contents of four terpenoids in wild and cultivate root of Tripterygium wilfordii Radix at different drying temperature
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Fig6: The contents of four terpenoids in wild and cultivate root of Tripterygium wilfordii Radix at different drying temperature

Mentions: During the different drying processes, the feasible drying temperature for root of Tripterygium wilfordii Radix was shown in Fig. 6. It was found that the highest content of triptolide in wild samples such as sample 2, 3, 6 and 7 was at 80 °C by comparing different temperatures. The highest content of triptolide in cultivate samples such as sample 4, 8, 9 and 13 was at 100 °C by comparing different temperatures. The highest content of triptophenolide in wild samples such as sample 2, 6, 7 and 10 was at 40 °C by comparing different temperatures. The highest content of triptophenolide in cultivate samples such as sample 4, 5, 8, 11 and 12 was at 80 °C by comparing different temperatures. The highest content of demethylzeylasteral in wild samples such as sample 3, 6 and 7 was at 40 °C which account for more than half in all wild samples by comparing different temperatures. The highest content of demethylzeylasteral in cultivate samples such as sample 4, 5, 9 and 13 was at 60 °C by comparing different temperatures. The highest content of celastrol in wild samples such as sample 1, 2, 3 and 7 was at 80 °C by comparing different temperatures. The highest content of celastrol in cultivate samples such as sample 4, 5, 8, 12 and 13 was at 60 °C by comparing different temperatures.Fig. 6


Comparative analysis of four terpenoids in root and cortex of Tripterygium wilfordii Radix by different drying methods
The contents of four terpenoids in wild and cultivate root of Tripterygium wilfordii Radix at different drying temperature
© Copyright Policy - OpenAccess
Related In: Results  -  Collection

License 1 - License 2
Show All Figures
getmorefigures.php?uid=PMC5120441&req=5

Fig6: The contents of four terpenoids in wild and cultivate root of Tripterygium wilfordii Radix at different drying temperature
Mentions: During the different drying processes, the feasible drying temperature for root of Tripterygium wilfordii Radix was shown in Fig. 6. It was found that the highest content of triptolide in wild samples such as sample 2, 3, 6 and 7 was at 80 °C by comparing different temperatures. The highest content of triptolide in cultivate samples such as sample 4, 8, 9 and 13 was at 100 °C by comparing different temperatures. The highest content of triptophenolide in wild samples such as sample 2, 6, 7 and 10 was at 40 °C by comparing different temperatures. The highest content of triptophenolide in cultivate samples such as sample 4, 5, 8, 11 and 12 was at 80 °C by comparing different temperatures. The highest content of demethylzeylasteral in wild samples such as sample 3, 6 and 7 was at 40 °C which account for more than half in all wild samples by comparing different temperatures. The highest content of demethylzeylasteral in cultivate samples such as sample 4, 5, 9 and 13 was at 60 °C by comparing different temperatures. The highest content of celastrol in wild samples such as sample 1, 2, 3 and 7 was at 80 °C by comparing different temperatures. The highest content of celastrol in cultivate samples such as sample 4, 5, 8, 12 and 13 was at 60 °C by comparing different temperatures.Fig. 6

View Article: PubMed Central - PubMed

ABSTRACT

Background: Tripterygium wilfordii Radix, a well-known traditional medicine in china which is used for treatment of inflammation, pain, tumor and immune regulation for centuries in china, accompany with the serious toxic side effects. This study was carried out for simultaneously analyzing the four main components (triptolide, triptophenolide, demethylzeylasteral and celastrol) in Tripterygium wilfordii Radix under different drying processes, which was important for reducing the toxicity and quality control of Tripterygium wilfordii Radix in future.

Methods: The terpenes were extracted by using ultrasonic method with ethyl acetate from root or cortex of Tripterygium wilfordii Radix, and the sensitive and rapid HPLC-PDA method was developed for simultaneous quantification of triptolide, triptophenolide, demethylzeylasteral and celastrol in root and cortex of Tripterygium wilfordii Radix for evaluation of the impacts by different drying processes.

Results: The four compounds in their respective determined arrange had good linearity of 0.9998≦R2≦0.9999 and the average recoveries were range from 94.69 to 100.28%, RSDs were within 0.27 to 2.42%, respectively. The contents of triptolide, triptophenolide, demethylzeylasteral and celastrol in different Tripterygium wilfordii Radix individuals were varied greatly at different drying temperatures. Under different temperatures, the contents of triptolide, triptophenolide, demethylzeylasteral, and celastrol were 37.94–70.31 mg/g, 0–1.807 mg/g, 0.3513–9.205 mg/g, 3.202–15.31 mg/g, respectively. The suitable drying temperature of terpenoids in root of wild and cultivate are 80 °C and 60 °C, the suitable drying temperature of terpenoids in cortex is 40 °C.

Conclusions: The method established is high sensitivity, accuracy, reliability and suitable for the simultaneous analysis of terpenoids in Tripterygium wilfordii Radix. The data provide a scientific basis and reference for the quality control of herb and preparations related to Tripterygium wilfordii Radix.

No MeSH data available.