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Combined detection of serum CTX-II and COMP concentrations in osteoarthritis model rabbits: an effective technique for early diagnosis and estimation of disease severity

View Article: PubMed Central - PubMed

ABSTRACT

Background: Early diagnosis of osteoarthritis (OA) is difficult. Cartilage oligomeric matrix protein (COMP) and crosslinked C-telopeptides of type II collagen (CTX-II) are two markers which can potentially predict the destruction of articular cartilage of early OA. To comprehensively evaluate the diagnosis value of serum COMP and CTX-II markers in OA, the longitudinal and combined measurement of serum COMP and CTX-II were performed at different stages of pathological process of OA in adult rabbits with OA, compared with the sham-operated rabbits.

Methods: Thirty-six adult white rabbits were randomly divided into two groups, the OA group and the control group (n = 18 per group). OA models were established by anterior cruciate ligament transection. Sham operations were performed in the control group. Before the surgery and at weeks 2, 4, 6, 8, 10, and 12 after surgery, serum CTX-II and COMP concentrations were detected using sandwich-ELISA in all rabbits. Three rabbits in each group were killed at weeks 2, 4, 6, 8, 10, and 12 after surgery, and femoral condyle specimens were collected. Histological changes in articular cartilage were evaluated according to the Mankin scoring criteria.

Results: At each time point, the Mankin scores and serum concentrations of CTX-II and COMP were significantly higher in the OA group than in the control group. In addition, in the OA group, there was a significant relationship between the CTX-II and COMP concentrations and the Mankin score.

Conclusions: Early dynamic combined detection of serum CTX-II and COMP concentrations is effective for early OA diagnosis and evaluation of OA severity.

No MeSH data available.


Mankin score of articular cartilage of rabbits in the OA and control groups at various time points
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Fig2: Mankin score of articular cartilage of rabbits in the OA and control groups at various time points

Mentions: In the control group, the slices of HE staining exhibited a smooth and intact surface of articular cartilage. In addition, the chondrocytes in the middle zone were organized and the staining of matrix was homogeneous (Fig. 1a). However, in the OA group, the different manifestations of articular cartilage were observed at various stages. At week 2 after surgery, the articular cartilage surface showed also smooth and intact. However, the chondrocyte in the superficial zone distributed unequally (Fig. 1b). At week 4 after surgery, the articular cartilage surface became unsmooth and the chondrocytes in the middle zone were disorganized (Fig. 1c). At week 6 after surgery, the articular surface showed completely rough, and the chondrocytes in the middle and deep zones were completely disorganized (Fig. 1d). At week 8 after surgery, proliferative chondrocyte clusters emerged in the superficial zone (Fig. 1e). At week 10 after surgery, proliferative chondrocyte clusters became noted in the superficial and middle zones. The matrix showed a loss of staining (Fig. 1f). At week 12 after surgery, vertical clefts in the calcified zone were found in some specimens. Cell loss was noted in the middle and deep zones (Fig. 1g). The Mankin scores are shown in Fig. 2. Within each time point, the Mankin scores in the OA group were significantly higher than those in the control group were (P < 0.05). The Mankin scores of the OA group increased as OA progressed.Fig. 1


Combined detection of serum CTX-II and COMP concentrations in osteoarthritis model rabbits: an effective technique for early diagnosis and estimation of disease severity
Mankin score of articular cartilage of rabbits in the OA and control groups at various time points
© Copyright Policy - OpenAccess
Related In: Results  -  Collection

License 1 - License 2
Show All Figures
getmorefigures.php?uid=PMC5120436&req=5

Fig2: Mankin score of articular cartilage of rabbits in the OA and control groups at various time points
Mentions: In the control group, the slices of HE staining exhibited a smooth and intact surface of articular cartilage. In addition, the chondrocytes in the middle zone were organized and the staining of matrix was homogeneous (Fig. 1a). However, in the OA group, the different manifestations of articular cartilage were observed at various stages. At week 2 after surgery, the articular cartilage surface showed also smooth and intact. However, the chondrocyte in the superficial zone distributed unequally (Fig. 1b). At week 4 after surgery, the articular cartilage surface became unsmooth and the chondrocytes in the middle zone were disorganized (Fig. 1c). At week 6 after surgery, the articular surface showed completely rough, and the chondrocytes in the middle and deep zones were completely disorganized (Fig. 1d). At week 8 after surgery, proliferative chondrocyte clusters emerged in the superficial zone (Fig. 1e). At week 10 after surgery, proliferative chondrocyte clusters became noted in the superficial and middle zones. The matrix showed a loss of staining (Fig. 1f). At week 12 after surgery, vertical clefts in the calcified zone were found in some specimens. Cell loss was noted in the middle and deep zones (Fig. 1g). The Mankin scores are shown in Fig. 2. Within each time point, the Mankin scores in the OA group were significantly higher than those in the control group were (P < 0.05). The Mankin scores of the OA group increased as OA progressed.Fig. 1

View Article: PubMed Central - PubMed

ABSTRACT

Background: Early diagnosis of osteoarthritis (OA) is difficult. Cartilage oligomeric matrix protein (COMP) and crosslinked C-telopeptides of type II collagen (CTX-II) are two markers which can potentially predict the destruction of articular cartilage of early OA. To comprehensively evaluate the diagnosis value of serum COMP and CTX-II markers in OA, the longitudinal and combined measurement of serum COMP and CTX-II were performed at different stages of pathological process of OA in adult rabbits with OA, compared with the sham-operated rabbits.

Methods: Thirty-six adult white rabbits were randomly divided into two groups, the OA group and the control group (n&thinsp;=&thinsp;18 per group). OA models were established by anterior cruciate ligament transection. Sham operations were performed in the control group. Before the surgery and at weeks 2, 4, 6, 8, 10, and 12 after surgery, serum CTX-II and COMP concentrations were detected using sandwich-ELISA in all rabbits. Three rabbits in each group were killed at weeks 2, 4, 6, 8, 10, and 12 after surgery, and femoral condyle specimens were collected. Histological changes in articular cartilage were evaluated according to the Mankin scoring criteria.

Results: At each time point, the Mankin scores and serum concentrations of CTX-II and COMP were significantly higher in the OA group than in the control group. In addition, in the OA group, there was a significant relationship between the CTX-II and COMP concentrations and the Mankin score.

Conclusions: Early dynamic combined detection of serum CTX-II and COMP concentrations is effective for early OA diagnosis and evaluation of OA severity.

No MeSH data available.