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CD163 macrophage and erythrocyte contents in aspirated deep vein thrombus are associated with the time after onset: a pilot study

View Article: PubMed Central - PubMed

ABSTRACT

Background: Thrombolytic therapy is effective in selected patients with deep vein thrombosis (DVT). Therefore, identification of a marker that reflects the age of thrombus is of particular concern. This pilot study aimed to identify a marker that reflects the time after onset in human aspirated DVT.

Methods: We histologically and immunohistochemically analyzed 16 aspirated thrombi. The times from onset to aspiration ranged from 5 to 60 days (median of 13 days). Paraffin sections were stained with hematoxylin and eosin and antibodies for fibrin, glycophorin A, integrin α2bβ3, macrophage markers (CD68, CD163, and CD206), CD34, and smooth muscle actin (SMA).

Results: All thrombi were immunopositive for glycophorin A, fibrin, integrin α2bβ3, CD68, CD163, and CD206, and contained granulocytes. Almost all of the thrombi had small foci of CD34- or SMA-immunopositive areas. CD68- and CD163-immunopositive cell numbers were positively correlated with the time after onset, while the glycophorin A-immunopositive area was negatively correlated with the time after onset. In double immunohistochemistry, CD163-positive cells existed predominantly among the CD68-immunopositive macrophage population. CD163-positive macrophages were closely localized with glycophorin A, CD34, or SMA-positive cell-rich areas.

Conclusions: These findings indicate that CD163 macrophage and erythrocyte contents could be markers for evaluation of the age of thrombus in DVT. Additionally, CD163 macrophages might play a role in organization of the process of venous thrombus.

Electronic supplementary material: The online version of this article (doi:10.1186/s12959-016-0122-0) contains supplementary material, which is available to authorized users.

No MeSH data available.


Related in: MedlinePlus

Localization of CD163 in aspirated deep vein thrombi. Double immunohistochemical staining for CD68 and CD163 (upper panel), glycophorin A and CD163 (middle panels), and CD163 and CD34 or SMA (lower panels). Expression of CD163 is visualized as a brown stain. Expression of CD68, glycophorin A, CD34, and SMA are visualized as a red stain. Most of the CD163-immunopositive cells (brown) are immunopositive for CD68 (red) (upper panel). CD163-immunopositive cells in erythrocyte-rich areas and phagocytosis of erythrocyte fragments can be seen (arrows, middle panels). Oval or stellate CD163-immunopositive cells in CD34- and SMA-immunopositive organizing areas can be seen (lower panels)
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Fig6: Localization of CD163 in aspirated deep vein thrombi. Double immunohistochemical staining for CD68 and CD163 (upper panel), glycophorin A and CD163 (middle panels), and CD163 and CD34 or SMA (lower panels). Expression of CD163 is visualized as a brown stain. Expression of CD68, glycophorin A, CD34, and SMA are visualized as a red stain. Most of the CD163-immunopositive cells (brown) are immunopositive for CD68 (red) (upper panel). CD163-immunopositive cells in erythrocyte-rich areas and phagocytosis of erythrocyte fragments can be seen (arrows, middle panels). Oval or stellate CD163-immunopositive cells in CD34- and SMA-immunopositive organizing areas can be seen (lower panels)

Mentions: Double immunohistochemical staining showed colocalization of CD68- and CD163-positive cells, accumulation of CD163-positive cells in erythrocyte-rich areas, and phagocytosis of erythrocyte fragments. Oval or stellate CD163-positive cells were also accumulated in CD34- and SMA-immunopositive areas (Fig. 6).Fig. 6


CD163 macrophage and erythrocyte contents in aspirated deep vein thrombus are associated with the time after onset: a pilot study
Localization of CD163 in aspirated deep vein thrombi. Double immunohistochemical staining for CD68 and CD163 (upper panel), glycophorin A and CD163 (middle panels), and CD163 and CD34 or SMA (lower panels). Expression of CD163 is visualized as a brown stain. Expression of CD68, glycophorin A, CD34, and SMA are visualized as a red stain. Most of the CD163-immunopositive cells (brown) are immunopositive for CD68 (red) (upper panel). CD163-immunopositive cells in erythrocyte-rich areas and phagocytosis of erythrocyte fragments can be seen (arrows, middle panels). Oval or stellate CD163-immunopositive cells in CD34- and SMA-immunopositive organizing areas can be seen (lower panels)
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Related In: Results  -  Collection

License 1 - License 2
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getmorefigures.php?uid=PMC5120412&req=5

Fig6: Localization of CD163 in aspirated deep vein thrombi. Double immunohistochemical staining for CD68 and CD163 (upper panel), glycophorin A and CD163 (middle panels), and CD163 and CD34 or SMA (lower panels). Expression of CD163 is visualized as a brown stain. Expression of CD68, glycophorin A, CD34, and SMA are visualized as a red stain. Most of the CD163-immunopositive cells (brown) are immunopositive for CD68 (red) (upper panel). CD163-immunopositive cells in erythrocyte-rich areas and phagocytosis of erythrocyte fragments can be seen (arrows, middle panels). Oval or stellate CD163-immunopositive cells in CD34- and SMA-immunopositive organizing areas can be seen (lower panels)
Mentions: Double immunohistochemical staining showed colocalization of CD68- and CD163-positive cells, accumulation of CD163-positive cells in erythrocyte-rich areas, and phagocytosis of erythrocyte fragments. Oval or stellate CD163-positive cells were also accumulated in CD34- and SMA-immunopositive areas (Fig. 6).Fig. 6

View Article: PubMed Central - PubMed

ABSTRACT

Background: Thrombolytic therapy is effective in selected patients with deep vein thrombosis (DVT). Therefore, identification of a marker that reflects the age of thrombus is of particular concern. This pilot study aimed to identify a marker that reflects the time after onset in human aspirated DVT.

Methods: We histologically and immunohistochemically analyzed 16 aspirated thrombi. The times from onset to aspiration ranged from 5 to 60 days (median of 13 days). Paraffin sections were stained with hematoxylin and eosin and antibodies for fibrin, glycophorin A, integrin α2bβ3, macrophage markers (CD68, CD163, and CD206), CD34, and smooth muscle actin (SMA).

Results: All thrombi were immunopositive for glycophorin A, fibrin, integrin α2bβ3, CD68, CD163, and CD206, and contained granulocytes. Almost all of the thrombi had small foci of CD34- or SMA-immunopositive areas. CD68- and CD163-immunopositive cell numbers were positively correlated with the time after onset, while the glycophorin A-immunopositive area was negatively correlated with the time after onset. In double immunohistochemistry, CD163-positive cells existed predominantly among the CD68-immunopositive macrophage population. CD163-positive macrophages were closely localized with glycophorin A, CD34, or SMA-positive cell-rich areas.

Conclusions: These findings indicate that CD163 macrophage and erythrocyte contents could be markers for evaluation of the age of thrombus in DVT. Additionally, CD163 macrophages might play a role in organization of the process of venous thrombus.

Electronic supplementary material: The online version of this article (doi:10.1186/s12959-016-0122-0) contains supplementary material, which is available to authorized users.

No MeSH data available.


Related in: MedlinePlus