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Antibacterial ability and hemocompatibility of graphene functionalized germanium

View Article: PubMed Central - PubMed

ABSTRACT

Germanium (Ge), as an elemental semiconductor material, has been an attractive candidate for manufacturing semiconductor microelectronic device. In the present investigation, to improve the biocompatibility of Ge-based device, graphene film is directly deposited on the Ge surface with different coverage area by controlling the growth time. Compared to bare Ge, the presence of graphene film entitles Ge with satisfactory antibacterial ability against Staphylococcus aureus (S.aureus), and acceptable antibacterial ability against Escherichia coli (E. coli). Meanwhile, antibacterial efficiency closely correlates with coverage area of graphene film, and larger graphene coverage always leads to better antibacterial performance. The underlying mechanism is thought to be the integrative action of phospholipids disturbance and electron extraction at the interface between graphene and biomembrane. Meanwhile, the electron extraction action would further lead to the activation of platelet. This study might provide some new insights into the relationship between antibacterial ability and hemocompatibility based on graphene functionalized biomedical device.

No MeSH data available.


Related in: MedlinePlus

(a and b) Representational SEM morphology of S.aureus (a) and E. coli (b) adhered on the surface with variable coverage area of graphene films after incubating for 24 h at both low (left panel) and high magnification (right panel), blue arrows indicate the crack and fracture.
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f4: (a and b) Representational SEM morphology of S.aureus (a) and E. coli (b) adhered on the surface with variable coverage area of graphene films after incubating for 24 h at both low (left panel) and high magnification (right panel), blue arrows indicate the crack and fracture.

Mentions: To further confirm the influence of the graphene coverage on the bacteria phenotype, SEM is utilized to inspect the membrane integrity and morphology of the two distinct bacteria, as displayed in Fig. 4. S.aureus on the None-Gr surface basically maintains original spherical appearance whereas those on the Half-Gr surface are disintegrated with severely distorted shape. Furthermore, the glomerate bacteria on the Full-Gr surface exhibit apparently deathlike state due to the crack of membrane. Meanwhile, the evident residue of exudative cytoplasm appears around the bacteria swarm. The similar manner is also observed for E. coli. On the None-Gr surface, the bacteria keep the typical rod-like shape. When the bacteria contact with Half-Gr surface, a minority of bacteria behaves with disrupted shape and loses the integrity of cell membrane while the majority of bacteria still remain the intrinsic shape. Nevertheless, the bacteria on the Full-Gr show irregular appearance characteristics, some bacteria have totally lost the cytoplasm ascribe to the fracture of membrane, which indicates poor living state. And, some robust bacteria with intact membrane still exist as well.


Antibacterial ability and hemocompatibility of graphene functionalized germanium
(a and b) Representational SEM morphology of S.aureus (a) and E. coli (b) adhered on the surface with variable coverage area of graphene films after incubating for 24 h at both low (left panel) and high magnification (right panel), blue arrows indicate the crack and fracture.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC5120343&req=5

f4: (a and b) Representational SEM morphology of S.aureus (a) and E. coli (b) adhered on the surface with variable coverage area of graphene films after incubating for 24 h at both low (left panel) and high magnification (right panel), blue arrows indicate the crack and fracture.
Mentions: To further confirm the influence of the graphene coverage on the bacteria phenotype, SEM is utilized to inspect the membrane integrity and morphology of the two distinct bacteria, as displayed in Fig. 4. S.aureus on the None-Gr surface basically maintains original spherical appearance whereas those on the Half-Gr surface are disintegrated with severely distorted shape. Furthermore, the glomerate bacteria on the Full-Gr surface exhibit apparently deathlike state due to the crack of membrane. Meanwhile, the evident residue of exudative cytoplasm appears around the bacteria swarm. The similar manner is also observed for E. coli. On the None-Gr surface, the bacteria keep the typical rod-like shape. When the bacteria contact with Half-Gr surface, a minority of bacteria behaves with disrupted shape and loses the integrity of cell membrane while the majority of bacteria still remain the intrinsic shape. Nevertheless, the bacteria on the Full-Gr show irregular appearance characteristics, some bacteria have totally lost the cytoplasm ascribe to the fracture of membrane, which indicates poor living state. And, some robust bacteria with intact membrane still exist as well.

View Article: PubMed Central - PubMed

ABSTRACT

Germanium (Ge), as an elemental semiconductor material, has been an attractive candidate for manufacturing semiconductor microelectronic device. In the present investigation, to improve the biocompatibility of Ge-based device, graphene film is directly deposited on the Ge surface with different coverage area by controlling the growth time. Compared to bare Ge, the presence of graphene film entitles Ge with satisfactory antibacterial ability against Staphylococcus aureus (S.aureus), and acceptable antibacterial ability against Escherichia coli (E. coli). Meanwhile, antibacterial efficiency closely correlates with coverage area of graphene film, and larger graphene coverage always leads to better antibacterial performance. The underlying mechanism is thought to be the integrative action of phospholipids disturbance and electron extraction at the interface between graphene and biomembrane. Meanwhile, the electron extraction action would further lead to the activation of platelet. This study might provide some new insights into the relationship between antibacterial ability and hemocompatibility based on graphene functionalized biomedical device.

No MeSH data available.


Related in: MedlinePlus